7M18

HeLa-tubulin in complex with cryptophycin 1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0003725	molecular_function	double-stranded RNA binding
A	0003924	molecular_function	GTPase activity
A	0005198	molecular_function	structural molecule activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0005881	cellular_component	cytoplasmic microtubule
A	0007017	biological_process	microtubule-based process
A	0015630	cellular_component	microtubule cytoskeleton
A	0016787	molecular_function	hydrolase activity
A	0030705	biological_process	cytoskeleton-dependent intracellular transport
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0051301	biological_process	cell division
A	0071353	biological_process	cellular response to interleukin-4
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0003924	molecular_function	GTPase activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0007017	biological_process	microtubule-based process
B	0007411	biological_process	axon guidance
B	0015630	cellular_component	microtubule cytoskeleton
B	0030027	cellular_component	lamellipodium
B	0030175	cellular_component	filopodium
B	0030182	biological_process	neuron differentiation
B	0030424	cellular_component	axon
B	0030425	cellular_component	dendrite
B	0030426	cellular_component	growth cone
B	0038007	biological_process	netrin-activated signaling pathway
B	0042277	molecular_function	peptide binding
B	0042995	cellular_component	cell projection
B	0043025	cellular_component	neuronal cell body
B	0045171	cellular_component	intercellular bridge
B	0046872	molecular_function	metal ion binding
B	0070062	cellular_component	extracellular exosome
B	0071944	cellular_component	cell periphery
B	0072686	cellular_component	mitotic spindle
B	1990791	biological_process	dorsal root ganglion development
B	1990890	molecular_function	netrin receptor binding
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000278	biological_process	mitotic cell cycle
C	0003725	molecular_function	double-stranded RNA binding
C	0003924	molecular_function	GTPase activity
C	0005198	molecular_function	structural molecule activity
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0005881	cellular_component	cytoplasmic microtubule
C	0007017	biological_process	microtubule-based process
C	0015630	cellular_component	microtubule cytoskeleton
C	0016787	molecular_function	hydrolase activity
C	0030705	biological_process	cytoskeleton-dependent intracellular transport
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0051301	biological_process	cell division
C	0071353	biological_process	cellular response to interleukin-4
D	0000226	biological_process	microtubule cytoskeleton organization
D	0000278	biological_process	mitotic cell cycle
D	0003924	molecular_function	GTPase activity
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005515	molecular_function	protein binding
D	0005525	molecular_function	GTP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005874	cellular_component	microtubule
D	0007017	biological_process	microtubule-based process
D	0007411	biological_process	axon guidance
D	0015630	cellular_component	microtubule cytoskeleton
D	0030027	cellular_component	lamellipodium
D	0030175	cellular_component	filopodium
D	0030182	biological_process	neuron differentiation
D	0030424	cellular_component	axon
D	0030425	cellular_component	dendrite
D	0030426	cellular_component	growth cone
D	0038007	biological_process	netrin-activated signaling pathway
D	0042277	molecular_function	peptide binding
D	0042995	cellular_component	cell projection
D	0043025	cellular_component	neuronal cell body
D	0045171	cellular_component	intercellular bridge
D	0046872	molecular_function	metal ion binding
D	0070062	cellular_component	extracellular exosome
D	0071944	cellular_component	cell periphery
D	0072686	cellular_component	mitotic spindle
D	1990791	biological_process	dorsal root ganglion development
D	1990890	molecular_function	netrin receptor binding
E	0000226	biological_process	microtubule cytoskeleton organization
E	0000278	biological_process	mitotic cell cycle
E	0003725	molecular_function	double-stranded RNA binding
E	0003924	molecular_function	GTPase activity
E	0005198	molecular_function	structural molecule activity
E	0005200	molecular_function	structural constituent of cytoskeleton
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005874	cellular_component	microtubule
E	0005881	cellular_component	cytoplasmic microtubule
E	0007017	biological_process	microtubule-based process
E	0015630	cellular_component	microtubule cytoskeleton
E	0016787	molecular_function	hydrolase activity
E	0030705	biological_process	cytoskeleton-dependent intracellular transport
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0051301	biological_process	cell division
E	0071353	biological_process	cellular response to interleukin-4
F	0000226	biological_process	microtubule cytoskeleton organization
F	0000278	biological_process	mitotic cell cycle
F	0003924	molecular_function	GTPase activity
F	0005200	molecular_function	structural constituent of cytoskeleton
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005874	cellular_component	microtubule
F	0007017	biological_process	microtubule-based process
F	0007411	biological_process	axon guidance
F	0015630	cellular_component	microtubule cytoskeleton
F	0030027	cellular_component	lamellipodium
F	0030175	cellular_component	filopodium
F	0030182	biological_process	neuron differentiation
F	0030424	cellular_component	axon
F	0030425	cellular_component	dendrite
F	0030426	cellular_component	growth cone
F	0038007	biological_process	netrin-activated signaling pathway
F	0042277	molecular_function	peptide binding
F	0042995	cellular_component	cell projection
F	0043025	cellular_component	neuronal cell body
F	0045171	cellular_component	intercellular bridge
F	0046872	molecular_function	metal ion binding
F	0070062	cellular_component	extracellular exosome
F	0071944	cellular_component	cell periphery
F	0072686	cellular_component	mitotic spindle
F	1990791	biological_process	dorsal root ganglion development
F	1990890	molecular_function	netrin receptor binding
G	0000226	biological_process	microtubule cytoskeleton organization
G	0000278	biological_process	mitotic cell cycle
G	0003725	molecular_function	double-stranded RNA binding
G	0003924	molecular_function	GTPase activity
G	0005198	molecular_function	structural molecule activity
G	0005200	molecular_function	structural constituent of cytoskeleton
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005737	cellular_component	cytoplasm
G	0005856	cellular_component	cytoskeleton
G	0005874	cellular_component	microtubule
G	0005881	cellular_component	cytoplasmic microtubule
G	0007017	biological_process	microtubule-based process
G	0015630	cellular_component	microtubule cytoskeleton
G	0016787	molecular_function	hydrolase activity
G	0030705	biological_process	cytoskeleton-dependent intracellular transport
G	0031625	molecular_function	ubiquitin protein ligase binding
G	0051301	biological_process	cell division
G	0071353	biological_process	cellular response to interleukin-4
H	0000226	biological_process	microtubule cytoskeleton organization
H	0000278	biological_process	mitotic cell cycle
H	0003924	molecular_function	GTPase activity
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005515	molecular_function	protein binding
H	0005525	molecular_function	GTP binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005874	cellular_component	microtubule
H	0007017	biological_process	microtubule-based process
H	0007411	biological_process	axon guidance
H	0015630	cellular_component	microtubule cytoskeleton
H	0030027	cellular_component	lamellipodium
H	0030175	cellular_component	filopodium
H	0030182	biological_process	neuron differentiation
H	0030424	cellular_component	axon
H	0030425	cellular_component	dendrite
H	0030426	cellular_component	growth cone
H	0038007	biological_process	netrin-activated signaling pathway
H	0042277	molecular_function	peptide binding
H	0042995	cellular_component	cell projection
H	0043025	cellular_component	neuronal cell body
H	0045171	cellular_component	intercellular bridge
H	0046872	molecular_function	metal ion binding
H	0070062	cellular_component	extracellular exosome
H	0071944	cellular_component	cell periphery
H	0072686	cellular_component	mitotic spindle
H	1990791	biological_process	dorsal root ganglion development
H	1990890	molecular_function	netrin receptor binding
I	0000226	biological_process	microtubule cytoskeleton organization
I	0000278	biological_process	mitotic cell cycle
I	0003725	molecular_function	double-stranded RNA binding
I	0003924	molecular_function	GTPase activity
I	0005198	molecular_function	structural molecule activity
I	0005200	molecular_function	structural constituent of cytoskeleton
I	0005515	molecular_function	protein binding
I	0005525	molecular_function	GTP binding
I	0005737	cellular_component	cytoplasm
I	0005856	cellular_component	cytoskeleton
I	0005874	cellular_component	microtubule
I	0005881	cellular_component	cytoplasmic microtubule
I	0007017	biological_process	microtubule-based process
I	0015630	cellular_component	microtubule cytoskeleton
I	0016787	molecular_function	hydrolase activity
I	0030705	biological_process	cytoskeleton-dependent intracellular transport
I	0031625	molecular_function	ubiquitin protein ligase binding
I	0051301	biological_process	cell division
I	0071353	biological_process	cellular response to interleukin-4
J	0000226	biological_process	microtubule cytoskeleton organization
J	0000278	biological_process	mitotic cell cycle
J	0003924	molecular_function	GTPase activity
J	0005200	molecular_function	structural constituent of cytoskeleton
J	0005515	molecular_function	protein binding
J	0005525	molecular_function	GTP binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005856	cellular_component	cytoskeleton
J	0005874	cellular_component	microtubule
J	0007017	biological_process	microtubule-based process
J	0007411	biological_process	axon guidance
J	0015630	cellular_component	microtubule cytoskeleton
J	0030027	cellular_component	lamellipodium
J	0030175	cellular_component	filopodium
J	0030182	biological_process	neuron differentiation
J	0030424	cellular_component	axon
J	0030425	cellular_component	dendrite
J	0030426	cellular_component	growth cone
J	0038007	biological_process	netrin-activated signaling pathway
J	0042277	molecular_function	peptide binding
J	0042995	cellular_component	cell projection
J	0043025	cellular_component	neuronal cell body
J	0045171	cellular_component	intercellular bridge
J	0046872	molecular_function	metal ion binding
J	0070062	cellular_component	extracellular exosome
J	0071944	cellular_component	cell periphery
J	0072686	cellular_component	mitotic spindle
J	1990791	biological_process	dorsal root ganglion development
J	1990890	molecular_function	netrin receptor binding
K	0000226	biological_process	microtubule cytoskeleton organization
K	0000278	biological_process	mitotic cell cycle
K	0003725	molecular_function	double-stranded RNA binding
K	0003924	molecular_function	GTPase activity
K	0005198	molecular_function	structural molecule activity
K	0005200	molecular_function	structural constituent of cytoskeleton
K	0005515	molecular_function	protein binding
K	0005525	molecular_function	GTP binding
K	0005737	cellular_component	cytoplasm
K	0005856	cellular_component	cytoskeleton
K	0005874	cellular_component	microtubule
K	0005881	cellular_component	cytoplasmic microtubule
K	0007017	biological_process	microtubule-based process
K	0015630	cellular_component	microtubule cytoskeleton
K	0016787	molecular_function	hydrolase activity
K	0030705	biological_process	cytoskeleton-dependent intracellular transport
K	0031625	molecular_function	ubiquitin protein ligase binding
K	0051301	biological_process	cell division
K	0071353	biological_process	cellular response to interleukin-4
L	0000226	biological_process	microtubule cytoskeleton organization
L	0000278	biological_process	mitotic cell cycle
L	0003924	molecular_function	GTPase activity
L	0005200	molecular_function	structural constituent of cytoskeleton
L	0005515	molecular_function	protein binding
L	0005525	molecular_function	GTP binding
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005856	cellular_component	cytoskeleton
L	0005874	cellular_component	microtubule
L	0007017	biological_process	microtubule-based process
L	0007411	biological_process	axon guidance
L	0015630	cellular_component	microtubule cytoskeleton
L	0030027	cellular_component	lamellipodium
L	0030175	cellular_component	filopodium
L	0030182	biological_process	neuron differentiation
L	0030424	cellular_component	axon
L	0030425	cellular_component	dendrite
L	0030426	cellular_component	growth cone
L	0038007	biological_process	netrin-activated signaling pathway
L	0042277	molecular_function	peptide binding
L	0042995	cellular_component	cell projection
L	0043025	cellular_component	neuronal cell body
L	0045171	cellular_component	intercellular bridge
L	0046872	molecular_function	metal ion binding
L	0070062	cellular_component	extracellular exosome
L	0071944	cellular_component	cell periphery
L	0072686	cellular_component	mitotic spindle
L	1990791	biological_process	dorsal root ganglion development
L	1990890	molecular_function	netrin receptor binding
M	0000226	biological_process	microtubule cytoskeleton organization
M	0000278	biological_process	mitotic cell cycle
M	0003725	molecular_function	double-stranded RNA binding
M	0003924	molecular_function	GTPase activity
M	0005198	molecular_function	structural molecule activity
M	0005200	molecular_function	structural constituent of cytoskeleton
M	0005515	molecular_function	protein binding
M	0005525	molecular_function	GTP binding
M	0005737	cellular_component	cytoplasm
M	0005856	cellular_component	cytoskeleton
M	0005874	cellular_component	microtubule
M	0005881	cellular_component	cytoplasmic microtubule
M	0007017	biological_process	microtubule-based process
M	0015630	cellular_component	microtubule cytoskeleton
M	0016787	molecular_function	hydrolase activity
M	0030705	biological_process	cytoskeleton-dependent intracellular transport
M	0031625	molecular_function	ubiquitin protein ligase binding
M	0051301	biological_process	cell division
M	0071353	biological_process	cellular response to interleukin-4
N	0000226	biological_process	microtubule cytoskeleton organization
N	0000278	biological_process	mitotic cell cycle
N	0003924	molecular_function	GTPase activity
N	0005200	molecular_function	structural constituent of cytoskeleton
N	0005515	molecular_function	protein binding
N	0005525	molecular_function	GTP binding
N	0005634	cellular_component	nucleus
N	0005737	cellular_component	cytoplasm
N	0005856	cellular_component	cytoskeleton
N	0005874	cellular_component	microtubule
N	0007017	biological_process	microtubule-based process
N	0007411	biological_process	axon guidance
N	0015630	cellular_component	microtubule cytoskeleton
N	0030027	cellular_component	lamellipodium
N	0030175	cellular_component	filopodium
N	0030182	biological_process	neuron differentiation
N	0030424	cellular_component	axon
N	0030425	cellular_component	dendrite
N	0030426	cellular_component	growth cone
N	0038007	biological_process	netrin-activated signaling pathway
N	0042277	molecular_function	peptide binding
N	0042995	cellular_component	cell projection
N	0043025	cellular_component	neuronal cell body
N	0045171	cellular_component	intercellular bridge
N	0046872	molecular_function	metal ion binding
N	0070062	cellular_component	extracellular exosome
N	0071944	cellular_component	cell periphery
N	0072686	cellular_component	mitotic spindle
N	1990791	biological_process	dorsal root ganglion development
N	1990890	molecular_function	netrin receptor binding
O	0000226	biological_process	microtubule cytoskeleton organization
O	0000278	biological_process	mitotic cell cycle
O	0003725	molecular_function	double-stranded RNA binding
O	0003924	molecular_function	GTPase activity
O	0005198	molecular_function	structural molecule activity
O	0005200	molecular_function	structural constituent of cytoskeleton
O	0005515	molecular_function	protein binding
O	0005525	molecular_function	GTP binding
O	0005737	cellular_component	cytoplasm
O	0005856	cellular_component	cytoskeleton
O	0005874	cellular_component	microtubule
O	0005881	cellular_component	cytoplasmic microtubule
O	0007017	biological_process	microtubule-based process
O	0015630	cellular_component	microtubule cytoskeleton
O	0016787	molecular_function	hydrolase activity
O	0030705	biological_process	cytoskeleton-dependent intracellular transport
O	0031625	molecular_function	ubiquitin protein ligase binding
O	0051301	biological_process	cell division
O	0071353	biological_process	cellular response to interleukin-4
P	0000226	biological_process	microtubule cytoskeleton organization
P	0000278	biological_process	mitotic cell cycle
P	0003924	molecular_function	GTPase activity
P	0005200	molecular_function	structural constituent of cytoskeleton
P	0005515	molecular_function	protein binding
P	0005525	molecular_function	GTP binding
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005856	cellular_component	cytoskeleton
P	0005874	cellular_component	microtubule
P	0007017	biological_process	microtubule-based process
P	0007411	biological_process	axon guidance
P	0015630	cellular_component	microtubule cytoskeleton
P	0030027	cellular_component	lamellipodium
P	0030175	cellular_component	filopodium
P	0030182	biological_process	neuron differentiation
P	0030424	cellular_component	axon
P	0030425	cellular_component	dendrite
P	0030426	cellular_component	growth cone
P	0038007	biological_process	netrin-activated signaling pathway
P	0042277	molecular_function	peptide binding
P	0042995	cellular_component	cell projection
P	0043025	cellular_component	neuronal cell body
P	0045171	cellular_component	intercellular bridge
P	0046872	molecular_function	metal ion binding
P	0070062	cellular_component	extracellular exosome
P	0071944	cellular_component	cell periphery
P	0072686	cellular_component	mitotic spindle
P	1990791	biological_process	dorsal root ganglion development
P	1990890	molecular_function	netrin receptor binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue YNP B 501

Chain	Residue
A	THR257
B	PHE394
B	TRP397
A	ASN258
A	MET313
A	CYS347
B	ASN99
B	ASN100
B	THR178
B	VAL179
B	VAL180

---

site_id	AC2
Number of Residues	13
Details	binding site for residue GDP B 502

Chain	Residue
B	GLN11
B	CYS12
B	ALA97
B	GLY140
B	GLY141
B	GLY142
B	GLY144
B	VAL169
B	SER176
B	ASN204
B	TYR222
B	LEU225
B	ASN226

---

site_id	AC3
Number of Residues	14
Details	binding site for residue GTP A 501

Chain	Residue
A	GLN11
A	ALA12
A	ASP98
A	ALA99
A	ALA100
A	ASN101
A	GLY143
A	GLY144
A	THR145
A	VAL177
A	GLU183
A	ASN206
A	TYR224
A	ASN228

---

site_id	AC4
Number of Residues	11
Details	binding site for residue YNP D 501

Chain	Residue
C	THR257
C	ASN258
C	MET313
C	CYS347
D	ASN99
D	ASN100
D	THR178
D	VAL179
D	VAL180
D	PHE394
D	TRP397

---

site_id	AC5
Number of Residues	13
Details	binding site for residue GDP D 502

Chain	Residue
D	GLN11
D	CYS12
D	ALA97
D	GLY140
D	GLY141
D	GLY142
D	GLY144
D	VAL169
D	SER176
D	ASN204
D	TYR222
D	LEU225
D	ASN226

---

site_id	AC6
Number of Residues	14
Details	binding site for residue GTP C 501

Chain	Residue
C	GLN11
C	ALA12
C	ASP98
C	ALA99
C	ALA100
C	ASN101
C	GLY143
C	GLY144
C	THR145
C	VAL177
C	GLU183
C	ASN206
C	TYR224
C	ASN228

---

site_id	AC7
Number of Residues	11
Details	binding site for residue YNP F 501

Chain	Residue
E	THR257
E	ASN258
E	MET313
E	CYS347
F	ASN99
F	ASN100
F	THR178
F	VAL179
F	VAL180
F	PHE394
F	TRP397

---

site_id	AC8
Number of Residues	13
Details	binding site for residue GDP F 502

Chain	Residue
F	ASN226
F	GLN11
F	CYS12
F	ALA97
F	GLY140
F	GLY141
F	GLY142
F	GLY144
F	VAL169
F	SER176
F	ASN204
F	TYR222
F	LEU225

---

site_id	AC9
Number of Residues	14
Details	binding site for residue GTP E 501

Chain	Residue
E	GLN11
E	ALA12
E	ASP98
E	ALA99
E	ALA100
E	ASN101
E	GLY143
E	GLY144
E	THR145
E	VAL177
E	GLU183
E	ASN206
E	TYR224
E	ASN228

---

site_id	AD1
Number of Residues	11
Details	binding site for residue YNP H 501

Chain	Residue
G	THR257
G	ASN258
G	MET313
G	CYS347
H	ASN99
H	ASN100
H	THR178
H	VAL179
H	VAL180
H	PHE394
H	TRP397

---

site_id	AD2
Number of Residues	13
Details	binding site for residue GDP H 502

Chain	Residue
H	GLN11
H	CYS12
H	ALA97
H	GLY140
H	GLY141
H	GLY142
H	GLY144
H	VAL169
H	SER176
H	ASN204
H	TYR222
H	LEU225
H	ASN226

---

site_id	AD3
Number of Residues	14
Details	binding site for residue GTP G 501

Chain	Residue
G	GLN11
G	ALA12
G	ASP98
G	ALA99
G	ALA100
G	ASN101
G	GLY143
G	GLY144
G	THR145
G	VAL177
G	GLU183
G	ASN206
G	TYR224
G	ASN228

---

site_id	AD4
Number of Residues	11
Details	binding site for residue YNP J 501

Chain	Residue
I	THR257
I	ASN258
I	MET313
I	CYS347
J	ASN99
J	ASN100
J	THR178
J	VAL179
J	VAL180
J	PHE394
J	TRP397

---

site_id	AD5
Number of Residues	13
Details	binding site for residue GDP J 502

Chain	Residue
J	GLN11
J	CYS12
J	ALA97
J	GLY140
J	GLY141
J	GLY142
J	GLY144
J	VAL169
J	SER176
J	ASN204
J	TYR222
J	LEU225
J	ASN226

---

site_id	AD6
Number of Residues	14
Details	binding site for residue GTP I 501

Chain	Residue
I	GLN11
I	ALA12
I	ASP98
I	ALA99
I	ALA100
I	ASN101
I	GLY143
I	GLY144
I	THR145
I	VAL177
I	GLU183
I	ASN206
I	TYR224
I	ASN228

---

site_id	AD7
Number of Residues	11
Details	binding site for residue YNP L 501

Chain	Residue
K	THR257
K	ASN258
K	MET313
K	CYS347
L	ASN99
L	ASN100
L	THR178
L	VAL179
L	VAL180
L	PHE394
L	TRP397

---

site_id	AD8
Number of Residues	13
Details	binding site for residue GDP L 502

Chain	Residue
L	GLN11
L	CYS12
L	ALA97
L	GLY140
L	GLY141
L	GLY142
L	GLY144
L	VAL169
L	SER176
L	ASN204
L	TYR222
L	LEU225
L	ASN226

---

site_id	AD9
Number of Residues	14
Details	binding site for residue GTP K 501

Chain	Residue
K	GLN11
K	ALA12
K	ASP98
K	ALA99
K	ALA100
K	ASN101
K	GLY143
K	GLY144
K	THR145
K	VAL177
K	GLU183
K	ASN206
K	TYR224
K	ASN228

---

site_id	AE1
Number of Residues	11
Details	binding site for residue YNP N 501

Chain	Residue
M	THR257
M	ASN258
M	MET313
M	CYS347
N	ASN99
N	ASN100
N	THR178
N	VAL179
N	VAL180
N	PHE394
N	TRP397

---

site_id	AE2
Number of Residues	13
Details	binding site for residue GDP N 502

Chain	Residue
N	GLN11
N	CYS12
N	ALA97
N	GLY140
N	GLY141
N	GLY142
N	GLY144
N	VAL169
N	SER176
N	ASN204
N	TYR222
N	LEU225
N	ASN226

---

site_id	AE3
Number of Residues	14
Details	binding site for residue GTP M 501

Chain	Residue
M	GLN11
M	ALA12
M	ASP98
M	ALA99
M	ALA100
M	ASN101
M	GLY143
M	GLY144
M	THR145
M	VAL177
M	GLU183
M	ASN206
M	TYR224
M	ASN228

---

site_id	AE4
Number of Residues	11
Details	binding site for residue YNP P 501

Chain	Residue
O	THR257
O	ASN258
O	MET313
O	CYS347
P	ASN99
P	ASN100
P	THR178
P	VAL179
P	VAL180
P	PHE394
P	TRP397

---

site_id	AE5
Number of Residues	13
Details	binding site for residue GDP P 502

Chain	Residue
P	GLN11
P	CYS12
P	ALA97
P	GLY140
P	GLY141
P	GLY142
P	GLY144
P	VAL169
P	SER176
P	ASN204
P	TYR222
P	LEU225
P	ASN226

---

site_id	AE6
Number of Residues	14
Details	binding site for residue GTP O 501

Chain	Residue
O	GLN11
O	ALA12
O	ASP98
O	ALA99
O	ALA100
O	ASN101
O	GLY143
O	GLY144
O	THR145
O	VAL177
O	GLU183
O	ASN206
O	TYR224
O	ASN228

---

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
A	GLY142-GLY148
B	GLY140-GLY146

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
B	MET1-ILE4

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:34996871

Chain	Residue	Details
A	GLU254
D	GLY144
D	ASN204
D	ASN226
F	GLN11
F	GLY142
F	THR143
F	GLY144
F	ASN204
F	ASN226
H	GLN11
C	GLU254
H	GLY142
H	THR143
H	GLY144
H	ASN204
H	ASN226
J	GLN11
J	GLY142
J	THR143
J	GLY144
J	ASN204
E	GLU254
J	ASN226
L	GLN11
L	GLY142
L	THR143
L	GLY144
L	ASN204
L	ASN226
N	GLN11
N	GLY142
N	THR143
G	GLU254
N	GLY144
N	ASN204
N	ASN226
P	GLN11
P	GLY142
P	THR143
P	GLY144
P	ASN204
P	ASN226
I	GLU254
K	GLU254
M	GLU254
O	GLU254
D	THR143

---

site_id	SWS_FT_FI2
Number of Residues	64
Details	BINDING: BINDING => ECO:0000269|PubMed:34996871, ECO:0000269|PubMed:35482892, ECO:0007744|PDB:7SJ7, ECO:0007744|PDB:7Z6S

Chain	Residue	Details
A	GLN11
C	GLU71
C	SER140
C	GLY144
C	THR145
C	THR179
C	ASN206
C	ASN228
E	GLN11
E	GLU71
E	SER140
A	GLU71
E	GLY144
E	THR145
E	THR179
E	ASN206
E	ASN228
G	GLN11
G	GLU71
G	SER140
G	GLY144
G	THR145
A	SER140
G	THR179
G	ASN206
G	ASN228
I	GLN11
I	GLU71
I	SER140
I	GLY144
I	THR145
I	THR179
I	ASN206
A	GLY144
I	ASN228
K	GLN11
K	GLU71
K	SER140
K	GLY144
K	THR145
K	THR179
K	ASN206
K	ASN228
M	GLN11
A	THR145
M	GLU71
M	SER140
M	GLY144
M	THR145
M	THR179
M	ASN206
M	ASN228
O	GLN11
O	GLU71
O	SER140
A	THR179
O	GLY144
O	THR145
O	THR179
O	ASN206
O	ASN228
A	ASN206
A	ASN228
C	GLN11

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	SITE: Involved in polymerization

Chain	Residue	Details
A	TYR451
C	TYR451
E	TYR451
G	TYR451
I	TYR451
K	TYR451
M	TYR451
O	TYR451

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000269|PubMed:24906155

Chain	Residue	Details
A	LYS40
C	LYS40
E	LYS40
G	LYS40
I	LYS40
K	LYS40
M	LYS40
O	LYS40

---

site_id	SWS_FT_FI5
Number of Residues	16
Details	MOD_RES: Phosphoserine => ECO:0000269|Ref.6

Chain	Residue	Details
A	SER48
I	SER232
K	SER48
K	SER232
M	SER48
M	SER232
O	SER48
O	SER232
A	SER232
C	SER48
C	SER232
E	SER48
E	SER232
G	SER48
G	SER232
I	SER48

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: 3'-nitrotyrosine => ECO:0000250|UniProtKB:P68373

Chain	Residue	Details
A	TYR282
C	TYR282
E	TYR282
G	TYR282
I	TYR282
K	TYR282
M	TYR282
O	TYR282

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: Omega-N-methylarginine => ECO:0000269|Ref.6

Chain	Residue	Details
A	ARG339
C	ARG339
E	ARG339
G	ARG339
I	ARG339
K	ARG339
M	ARG339
O	ARG339

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P68373

Chain	Residue	Details
A	SER439
C	SER439
E	SER439
G	SER439
I	SER439
K	SER439
M	SER439
O	SER439

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000269|PubMed:32747782

Chain	Residue	Details
A	GLU443
C	GLU443
E	GLU443
G	GLU443
I	GLU443
K	GLU443
M	GLU443
O	GLU443

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:P68369

Chain	Residue	Details
A	GLU445
C	GLU445
E	GLU445
G	GLU445
I	GLU445
K	GLU445
M	GLU445
O	GLU445

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	MOD_RES: 3'-nitrotyrosine => ECO:0000269|PubMed:10339593

Chain	Residue	Details
A	TYR451
C	TYR451
E	TYR451
G	TYR451
I	TYR451
K	TYR451
M	TYR451
O	TYR451

---

site_id	SWS_FT_FI12
Number of Residues	24
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
A	LYS326
G	LYS326
G	LYS370
I	LYS326
I	LYS370
K	LYS326
K	LYS370
M	LYS326
M	LYS370
O	LYS326
O	LYS370
A	LYS370
C	LYS326
C	LYS370
E	LYS326
E	LYS370

---