7KO7

Structure of the native cardiac thin filament at pCa=5.8 having upper Tn in Ca2+ free state and lower Tn in Ca2+ bound state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001725	cellular_component	stress fiber
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005865	cellular_component	striated muscle thin filament
A	0005884	cellular_component	actin filament
A	0010628	biological_process	positive regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0030027	cellular_component	lamellipodium
A	0030175	cellular_component	filopodium
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0044297	cellular_component	cell body
A	0048741	biological_process	skeletal muscle fiber development
A	0090131	biological_process	mesenchyme migration
B	0000166	molecular_function	nucleotide binding
B	0001725	cellular_component	stress fiber
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0005865	cellular_component	striated muscle thin filament
B	0005884	cellular_component	actin filament
B	0010628	biological_process	positive regulation of gene expression
B	0016787	molecular_function	hydrolase activity
B	0030027	cellular_component	lamellipodium
B	0030175	cellular_component	filopodium
B	0030240	biological_process	skeletal muscle thin filament assembly
B	0044297	cellular_component	cell body
B	0048741	biological_process	skeletal muscle fiber development
B	0090131	biological_process	mesenchyme migration
C	0000166	molecular_function	nucleotide binding
C	0001725	cellular_component	stress fiber
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005865	cellular_component	striated muscle thin filament
C	0005884	cellular_component	actin filament
C	0010628	biological_process	positive regulation of gene expression
C	0016787	molecular_function	hydrolase activity
C	0030027	cellular_component	lamellipodium
C	0030175	cellular_component	filopodium
C	0030240	biological_process	skeletal muscle thin filament assembly
C	0044297	cellular_component	cell body
C	0048741	biological_process	skeletal muscle fiber development
C	0090131	biological_process	mesenchyme migration
D	0000166	molecular_function	nucleotide binding
D	0001725	cellular_component	stress fiber
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0005865	cellular_component	striated muscle thin filament
D	0005884	cellular_component	actin filament
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0030027	cellular_component	lamellipodium
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0044297	cellular_component	cell body
D	0048741	biological_process	skeletal muscle fiber development
D	0090131	biological_process	mesenchyme migration
E	0000166	molecular_function	nucleotide binding
E	0001725	cellular_component	stress fiber
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005865	cellular_component	striated muscle thin filament
E	0005884	cellular_component	actin filament
E	0010628	biological_process	positive regulation of gene expression
E	0016787	molecular_function	hydrolase activity
E	0030027	cellular_component	lamellipodium
E	0030175	cellular_component	filopodium
E	0030240	biological_process	skeletal muscle thin filament assembly
E	0044297	cellular_component	cell body
E	0048741	biological_process	skeletal muscle fiber development
E	0090131	biological_process	mesenchyme migration
F	0000166	molecular_function	nucleotide binding
F	0001725	cellular_component	stress fiber
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005865	cellular_component	striated muscle thin filament
F	0005884	cellular_component	actin filament
F	0010628	biological_process	positive regulation of gene expression
F	0016787	molecular_function	hydrolase activity
F	0030027	cellular_component	lamellipodium
F	0030175	cellular_component	filopodium
F	0030240	biological_process	skeletal muscle thin filament assembly
F	0044297	cellular_component	cell body
F	0048741	biological_process	skeletal muscle fiber development
F	0090131	biological_process	mesenchyme migration
G	0000166	molecular_function	nucleotide binding
G	0001725	cellular_component	stress fiber
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005865	cellular_component	striated muscle thin filament
G	0005884	cellular_component	actin filament
G	0010628	biological_process	positive regulation of gene expression
G	0016787	molecular_function	hydrolase activity
G	0030027	cellular_component	lamellipodium
G	0030175	cellular_component	filopodium
G	0030240	biological_process	skeletal muscle thin filament assembly
G	0044297	cellular_component	cell body
G	0048741	biological_process	skeletal muscle fiber development
G	0090131	biological_process	mesenchyme migration
H	0000166	molecular_function	nucleotide binding
H	0001725	cellular_component	stress fiber
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0005865	cellular_component	striated muscle thin filament
H	0005884	cellular_component	actin filament
H	0010628	biological_process	positive regulation of gene expression
H	0016787	molecular_function	hydrolase activity
H	0030027	cellular_component	lamellipodium
H	0030175	cellular_component	filopodium
H	0030240	biological_process	skeletal muscle thin filament assembly
H	0044297	cellular_component	cell body
H	0048741	biological_process	skeletal muscle fiber development
H	0090131	biological_process	mesenchyme migration
I	0000166	molecular_function	nucleotide binding
I	0001725	cellular_component	stress fiber
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005856	cellular_component	cytoskeleton
I	0005865	cellular_component	striated muscle thin filament
I	0005884	cellular_component	actin filament
I	0010628	biological_process	positive regulation of gene expression
I	0016787	molecular_function	hydrolase activity
I	0030027	cellular_component	lamellipodium
I	0030175	cellular_component	filopodium
I	0030240	biological_process	skeletal muscle thin filament assembly
I	0044297	cellular_component	cell body
I	0048741	biological_process	skeletal muscle fiber development
I	0090131	biological_process	mesenchyme migration
J	0000166	molecular_function	nucleotide binding
J	0001725	cellular_component	stress fiber
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005856	cellular_component	cytoskeleton
J	0005865	cellular_component	striated muscle thin filament
J	0005884	cellular_component	actin filament
J	0010628	biological_process	positive regulation of gene expression
J	0016787	molecular_function	hydrolase activity
J	0030027	cellular_component	lamellipodium
J	0030175	cellular_component	filopodium
J	0030240	biological_process	skeletal muscle thin filament assembly
J	0044297	cellular_component	cell body
J	0048741	biological_process	skeletal muscle fiber development
J	0090131	biological_process	mesenchyme migration
K	0000166	molecular_function	nucleotide binding
K	0001725	cellular_component	stress fiber
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005856	cellular_component	cytoskeleton
K	0005865	cellular_component	striated muscle thin filament
K	0005884	cellular_component	actin filament
K	0010628	biological_process	positive regulation of gene expression
K	0016787	molecular_function	hydrolase activity
K	0030027	cellular_component	lamellipodium
K	0030175	cellular_component	filopodium
K	0030240	biological_process	skeletal muscle thin filament assembly
K	0044297	cellular_component	cell body
K	0048741	biological_process	skeletal muscle fiber development
K	0090131	biological_process	mesenchyme migration
L	0000166	molecular_function	nucleotide binding
L	0001725	cellular_component	stress fiber
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005856	cellular_component	cytoskeleton
L	0005865	cellular_component	striated muscle thin filament
L	0005884	cellular_component	actin filament
L	0010628	biological_process	positive regulation of gene expression
L	0016787	molecular_function	hydrolase activity
L	0030027	cellular_component	lamellipodium
L	0030175	cellular_component	filopodium
L	0030240	biological_process	skeletal muscle thin filament assembly
L	0044297	cellular_component	cell body
L	0048741	biological_process	skeletal muscle fiber development
L	0090131	biological_process	mesenchyme migration
M	0000166	molecular_function	nucleotide binding
M	0001725	cellular_component	stress fiber
M	0005524	molecular_function	ATP binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005856	cellular_component	cytoskeleton
M	0005865	cellular_component	striated muscle thin filament
M	0005884	cellular_component	actin filament
M	0010628	biological_process	positive regulation of gene expression
M	0016787	molecular_function	hydrolase activity
M	0030027	cellular_component	lamellipodium
M	0030175	cellular_component	filopodium
M	0030240	biological_process	skeletal muscle thin filament assembly
M	0044297	cellular_component	cell body
M	0048741	biological_process	skeletal muscle fiber development
M	0090131	biological_process	mesenchyme migration
N	0000166	molecular_function	nucleotide binding
N	0001725	cellular_component	stress fiber
N	0005524	molecular_function	ATP binding
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005856	cellular_component	cytoskeleton
N	0005865	cellular_component	striated muscle thin filament
N	0005884	cellular_component	actin filament
N	0010628	biological_process	positive regulation of gene expression
N	0016787	molecular_function	hydrolase activity
N	0030027	cellular_component	lamellipodium
N	0030175	cellular_component	filopodium
N	0030240	biological_process	skeletal muscle thin filament assembly
N	0044297	cellular_component	cell body
N	0048741	biological_process	skeletal muscle fiber development
N	0090131	biological_process	mesenchyme migration
O	0000166	molecular_function	nucleotide binding
O	0001725	cellular_component	stress fiber
O	0005524	molecular_function	ATP binding
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0005865	cellular_component	striated muscle thin filament
O	0005884	cellular_component	actin filament
O	0010628	biological_process	positive regulation of gene expression
O	0016787	molecular_function	hydrolase activity
O	0030027	cellular_component	lamellipodium
O	0030175	cellular_component	filopodium
O	0030240	biological_process	skeletal muscle thin filament assembly
O	0044297	cellular_component	cell body
O	0048741	biological_process	skeletal muscle fiber development
O	0090131	biological_process	mesenchyme migration
P	0003779	molecular_function	actin binding
P	0005856	cellular_component	cytoskeleton
P	0005884	cellular_component	actin filament
P	0007015	biological_process	actin filament organization
P	0015629	cellular_component	actin cytoskeleton
P	0042802	molecular_function	identical protein binding
P	0042803	molecular_function	protein homodimerization activity
P	0046982	molecular_function	protein heterodimerization activity
P	0051015	molecular_function	actin filament binding
P	0060048	biological_process	cardiac muscle contraction
Q	0003779	molecular_function	actin binding
Q	0005856	cellular_component	cytoskeleton
Q	0005884	cellular_component	actin filament
Q	0007015	biological_process	actin filament organization
Q	0015629	cellular_component	actin cytoskeleton
Q	0042802	molecular_function	identical protein binding
Q	0042803	molecular_function	protein homodimerization activity
Q	0046982	molecular_function	protein heterodimerization activity
Q	0051015	molecular_function	actin filament binding
Q	0060048	biological_process	cardiac muscle contraction
R	0003779	molecular_function	actin binding
R	0005856	cellular_component	cytoskeleton
R	0005884	cellular_component	actin filament
R	0007015	biological_process	actin filament organization
R	0015629	cellular_component	actin cytoskeleton
R	0042802	molecular_function	identical protein binding
R	0042803	molecular_function	protein homodimerization activity
R	0046982	molecular_function	protein heterodimerization activity
R	0051015	molecular_function	actin filament binding
R	0060048	biological_process	cardiac muscle contraction
S	0003779	molecular_function	actin binding
S	0005856	cellular_component	cytoskeleton
S	0005884	cellular_component	actin filament
S	0007015	biological_process	actin filament organization
S	0015629	cellular_component	actin cytoskeleton
S	0042802	molecular_function	identical protein binding
S	0042803	molecular_function	protein homodimerization activity
S	0046982	molecular_function	protein heterodimerization activity
S	0051015	molecular_function	actin filament binding
S	0060048	biological_process	cardiac muscle contraction
T	0005861	cellular_component	troponin complex
T	0006937	biological_process	regulation of muscle contraction
U	0005861	cellular_component	troponin complex
V	0003009	biological_process	skeletal muscle contraction
V	0005509	molecular_function	calcium ion binding
V	0005861	cellular_component	troponin complex
V	0006937	biological_process	regulation of muscle contraction
V	0008092	molecular_function	cytoskeletal protein binding
V	0031013	molecular_function	troponin I binding
V	0031014	molecular_function	troponin T binding
V	0032972	biological_process	regulation of muscle filament sliding speed
V	0042803	molecular_function	protein homodimerization activity
V	0046872	molecular_function	metal ion binding
V	0048306	molecular_function	calcium-dependent protein binding
V	0051015	molecular_function	actin filament binding
V	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
V	0060048	biological_process	cardiac muscle contraction
V	0086003	biological_process	cardiac muscle cell contraction
V	1990584	cellular_component	cardiac Troponin complex
W	0003779	molecular_function	actin binding
W	0005856	cellular_component	cytoskeleton
W	0005884	cellular_component	actin filament
W	0007015	biological_process	actin filament organization
W	0015629	cellular_component	actin cytoskeleton
W	0042802	molecular_function	identical protein binding
W	0042803	molecular_function	protein homodimerization activity
W	0046982	molecular_function	protein heterodimerization activity
W	0051015	molecular_function	actin filament binding
W	0060048	biological_process	cardiac muscle contraction
X	0003779	molecular_function	actin binding
X	0005856	cellular_component	cytoskeleton
X	0005884	cellular_component	actin filament
X	0007015	biological_process	actin filament organization
X	0015629	cellular_component	actin cytoskeleton
X	0042802	molecular_function	identical protein binding
X	0042803	molecular_function	protein homodimerization activity
X	0046982	molecular_function	protein heterodimerization activity
X	0051015	molecular_function	actin filament binding
X	0060048	biological_process	cardiac muscle contraction
Y	0003779	molecular_function	actin binding
Y	0005856	cellular_component	cytoskeleton
Y	0005884	cellular_component	actin filament
Y	0007015	biological_process	actin filament organization
Y	0015629	cellular_component	actin cytoskeleton
Y	0042802	molecular_function	identical protein binding
Y	0042803	molecular_function	protein homodimerization activity
Y	0046982	molecular_function	protein heterodimerization activity
Y	0051015	molecular_function	actin filament binding
Y	0060048	biological_process	cardiac muscle contraction
Z	0003779	molecular_function	actin binding
Z	0005856	cellular_component	cytoskeleton
Z	0005884	cellular_component	actin filament
Z	0007015	biological_process	actin filament organization
Z	0015629	cellular_component	actin cytoskeleton
Z	0042802	molecular_function	identical protein binding
Z	0042803	molecular_function	protein homodimerization activity
Z	0046982	molecular_function	protein heterodimerization activity
Z	0051015	molecular_function	actin filament binding
Z	0060048	biological_process	cardiac muscle contraction
a	0005861	cellular_component	troponin complex
a	0006937	biological_process	regulation of muscle contraction
b	0005861	cellular_component	troponin complex
c	0003009	biological_process	skeletal muscle contraction
c	0005509	molecular_function	calcium ion binding
c	0005861	cellular_component	troponin complex
c	0006937	biological_process	regulation of muscle contraction
c	0008092	molecular_function	cytoskeletal protein binding
c	0031013	molecular_function	troponin I binding
c	0031014	molecular_function	troponin T binding
c	0032972	biological_process	regulation of muscle filament sliding speed
c	0042803	molecular_function	protein homodimerization activity
c	0046872	molecular_function	metal ion binding
c	0048306	molecular_function	calcium-dependent protein binding
c	0051015	molecular_function	actin filament binding
c	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
c	0060048	biological_process	cardiac muscle contraction
c	0086003	biological_process	cardiac muscle cell contraction
c	1990584	cellular_component	cardiac Troponin complex

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DEDGSGTVDfdEF

Chain	Residue	Details
c	ASP65-PHE77
c	ASP105-LEU117
c	ASP141-PHE153

---

site_id	PS00326
Number of Residues	9
Details	TROPOMYOSIN Tropomyosins signature. LKEAEtRAE

Chain	Residue	Details
P	LEU232-GLU240

---

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

---

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
A	TRP356-GLU364

---

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	375
Details	Region: {"description":"Interaction with alpha-actinin","evidences":[{"source":"UniProtKB","id":"P68135","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	30
Details	Modified residue: {"description":"Methionine (R)-sulfoxide","evidences":[{"source":"UniProtKB","id":"P68134","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	15
Details	Modified residue: {"description":"N6-malonyllysine","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	15
Details	Modified residue: {"description":"Tele-methylhistidine","evidences":[{"source":"UniProtKB","id":"P68138","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	15
Details	Modified residue: {"description":"N6-methyllysine","evidences":[{"source":"UniProtKB","id":"P68133","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	316
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	108
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P04692","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P09493","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	16
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P58771","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P04692","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine; by DAPK1","evidences":[{"source":"UniProtKB","id":"P09493","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"UniProtKB","id":"P09493","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	70
Details	Domain: {"description":"EF-hand 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	70
Details	Domain: {"description":"EF-hand 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	70
Details	Domain: {"description":"EF-hand 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	66
Details	Domain: {"description":"EF-hand 4","evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	30
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P19123","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---