7KO7
Structure of the native cardiac thin filament at pCa=5.8 having upper Tn in Ca2+ free state and lower Tn in Ca2+ bound state
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 8.3 |
Software | RELION,UCSF Chimera,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |