7K62
Crystal Structure of Dihydrofolate reductase from Mycobacterium kansasii in complex with NADP and inhibitor P218
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0044281 | biological_process | small molecule metabolic process |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070401 | molecular_function | NADP+ binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0044281 | biological_process | small molecule metabolic process |
| B | 0046452 | biological_process | dihydrofolate metabolic process |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0046655 | biological_process | folic acid metabolic process |
| B | 0050661 | molecular_function | NADP binding |
| B | 0070401 | molecular_function | NADP+ binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 29 |
| Details | binding site for residue NAP A 201 |
| Chain | Residue |
| A | TRP5 |
| A | LEU64 |
| A | SER65 |
| A | ARG66 |
| A | GLN67 |
| A | GLY79 |
| A | ILE94 |
| A | GLY95 |
| A | GLY96 |
| A | GLU97 |
| A | GLN98 |
| A | ALA6 |
| A | ILE99 |
| A | TYR100 |
| A | LEU102 |
| A | ALA126 |
| A | MMV202 |
| A | HOH315 |
| A | HOH317 |
| A | HOH327 |
| A | HOH340 |
| A | HOH349 |
| A | ILE13 |
| A | GLY17 |
| A | ASP18 |
| A | GLY42 |
| A | ARG43 |
| A | ARG44 |
| A | THR45 |
| site_id | AC2 |
| Number of Residues | 15 |
| Details | binding site for residue MMV A 202 |
| Chain | Residue |
| A | ILE4 |
| A | TRP5 |
| A | ALA6 |
| A | ILE19 |
| A | ASP26 |
| A | PHE30 |
| A | LYS31 |
| A | LEU49 |
| A | ARG59 |
| A | ILE94 |
| A | TYR100 |
| A | TYR157 |
| A | NAP201 |
| A | HOH321 |
| A | HOH332 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | binding site for residue EDO A 203 |
| Chain | Residue |
| A | TRP21 |
| A | VAL23 |
| site_id | AC4 |
| Number of Residues | 26 |
| Details | binding site for residue NAP B 201 |
| Chain | Residue |
| B | TRP5 |
| B | ALA6 |
| B | ILE13 |
| B | GLY17 |
| B | ASP18 |
| B | ILE19 |
| B | GLY42 |
| B | ARG43 |
| B | ARG44 |
| B | THR45 |
| B | LEU64 |
| B | SER65 |
| B | ARG66 |
| B | GLN67 |
| B | GLY79 |
| B | ILE94 |
| B | GLY95 |
| B | GLY96 |
| B | GLU97 |
| B | GLN98 |
| B | ILE99 |
| B | TYR100 |
| B | LEU102 |
| B | MMV202 |
| B | HOH304 |
| B | HOH310 |
| site_id | AC5 |
| Number of Residues | 14 |
| Details | binding site for residue MMV B 202 |
| Chain | Residue |
| B | ILE4 |
| B | TRP5 |
| B | ALA6 |
| B | ILE19 |
| B | ASP26 |
| B | PHE30 |
| B | LYS31 |
| B | LEU49 |
| B | ARG59 |
| B | ILE94 |
| B | TYR100 |
| B | TYR157 |
| B | NAP201 |
| B | HOH307 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for residue EDO B 203 |
| Chain | Residue |
| B | TRP21 |
| B | VAL23 |
| B | HOH309 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGrggdIPWrvpe.DlshFkriT |
| Chain | Residue | Details |
| A | VAL12-THR34 |
