Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7DBK

Crystal structure of human LDHB in complex with NADH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004457	molecular_function	lactate dehydrogenase activity
A	0004459	molecular_function	L-lactate dehydrogenase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005829	cellular_component	cytosol
A	0006089	biological_process	lactate metabolic process
A	0006090	biological_process	pyruvate metabolic process
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019674	biological_process	NAD metabolic process
A	0019752	biological_process	carboxylic acid metabolic process
A	0019900	molecular_function	kinase binding
A	0042802	molecular_function	identical protein binding
A	0045121	cellular_component	membrane raft
A	0051287	molecular_function	NAD binding
A	0070062	cellular_component	extracellular exosome
A	1990204	cellular_component	oxidoreductase complex
B	0003824	molecular_function	catalytic activity
B	0004457	molecular_function	lactate dehydrogenase activity
B	0004459	molecular_function	L-lactate dehydrogenase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005829	cellular_component	cytosol
B	0006089	biological_process	lactate metabolic process
B	0006090	biological_process	pyruvate metabolic process
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019674	biological_process	NAD metabolic process
B	0019752	biological_process	carboxylic acid metabolic process
B	0019900	molecular_function	kinase binding
B	0042802	molecular_function	identical protein binding
B	0045121	cellular_component	membrane raft
B	0051287	molecular_function	NAD binding
B	0070062	cellular_component	extracellular exosome
B	1990204	cellular_component	oxidoreductase complex
C	0003824	molecular_function	catalytic activity
C	0004457	molecular_function	lactate dehydrogenase activity
C	0004459	molecular_function	L-lactate dehydrogenase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0005829	cellular_component	cytosol
C	0006089	biological_process	lactate metabolic process
C	0006090	biological_process	pyruvate metabolic process
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019674	biological_process	NAD metabolic process
C	0019752	biological_process	carboxylic acid metabolic process
C	0019900	molecular_function	kinase binding
C	0042802	molecular_function	identical protein binding
C	0045121	cellular_component	membrane raft
C	0051287	molecular_function	NAD binding
C	0070062	cellular_component	extracellular exosome
C	1990204	cellular_component	oxidoreductase complex
D	0003824	molecular_function	catalytic activity
D	0004457	molecular_function	lactate dehydrogenase activity
D	0004459	molecular_function	L-lactate dehydrogenase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0005829	cellular_component	cytosol
D	0006089	biological_process	lactate metabolic process
D	0006090	biological_process	pyruvate metabolic process
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0019674	biological_process	NAD metabolic process
D	0019752	biological_process	carboxylic acid metabolic process
D	0019900	molecular_function	kinase binding
D	0042802	molecular_function	identical protein binding
D	0045121	cellular_component	membrane raft
D	0051287	molecular_function	NAD binding
D	0070062	cellular_component	extracellular exosome
D	1990204	cellular_component	oxidoreductase complex
E	0003824	molecular_function	catalytic activity
E	0004457	molecular_function	lactate dehydrogenase activity
E	0004459	molecular_function	L-lactate dehydrogenase activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0005829	cellular_component	cytosol
E	0006089	biological_process	lactate metabolic process
E	0006090	biological_process	pyruvate metabolic process
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0019674	biological_process	NAD metabolic process
E	0019752	biological_process	carboxylic acid metabolic process
E	0019900	molecular_function	kinase binding
E	0042802	molecular_function	identical protein binding
E	0045121	cellular_component	membrane raft
E	0051287	molecular_function	NAD binding
E	0070062	cellular_component	extracellular exosome
E	1990204	cellular_component	oxidoreductase complex
F	0003824	molecular_function	catalytic activity
F	0004457	molecular_function	lactate dehydrogenase activity
F	0004459	molecular_function	L-lactate dehydrogenase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0005829	cellular_component	cytosol
F	0006089	biological_process	lactate metabolic process
F	0006090	biological_process	pyruvate metabolic process
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0019674	biological_process	NAD metabolic process
F	0019752	biological_process	carboxylic acid metabolic process
F	0019900	molecular_function	kinase binding
F	0042802	molecular_function	identical protein binding
F	0045121	cellular_component	membrane raft
F	0051287	molecular_function	NAD binding
F	0070062	cellular_component	extracellular exosome
F	1990204	cellular_component	oxidoreductase complex
G	0003824	molecular_function	catalytic activity
G	0004457	molecular_function	lactate dehydrogenase activity
G	0004459	molecular_function	L-lactate dehydrogenase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005743	cellular_component	mitochondrial inner membrane
G	0005829	cellular_component	cytosol
G	0006089	biological_process	lactate metabolic process
G	0006090	biological_process	pyruvate metabolic process
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0019674	biological_process	NAD metabolic process
G	0019752	biological_process	carboxylic acid metabolic process
G	0019900	molecular_function	kinase binding
G	0042802	molecular_function	identical protein binding
G	0045121	cellular_component	membrane raft
G	0051287	molecular_function	NAD binding
G	0070062	cellular_component	extracellular exosome
G	1990204	cellular_component	oxidoreductase complex
H	0003824	molecular_function	catalytic activity
H	0004457	molecular_function	lactate dehydrogenase activity
H	0004459	molecular_function	L-lactate dehydrogenase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005739	cellular_component	mitochondrion
H	0005743	cellular_component	mitochondrial inner membrane
H	0005829	cellular_component	cytosol
H	0006089	biological_process	lactate metabolic process
H	0006090	biological_process	pyruvate metabolic process
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0019674	biological_process	NAD metabolic process
H	0019752	biological_process	carboxylic acid metabolic process
H	0019900	molecular_function	kinase binding
H	0042802	molecular_function	identical protein binding
H	0045121	cellular_component	membrane raft
H	0051287	molecular_function	NAD binding
H	0070062	cellular_component	extracellular exosome
H	1990204	cellular_component	oxidoreductase complex

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. LGEHGDS

Chain	Residue	Details
A	LEU191-SER197

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	HIS194
B	HIS194
C	HIS194
D	HIS194
E	HIS194
F	HIS194
G	HIS194
H	HIS194

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	GLN31
B	GLN31
C	GLN31
D	GLN31
E	GLN31
F	GLN31
G	GLN31
H	GLN31

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:11276087

Chain	Residue	Details
A	ARG100
B	ARG100
C	ARG100
D	ARG100
E	ARG100
F	ARG100
G	ARG100
H	ARG100

site_id	SWS_FT_FI4
Number of Residues	32
Details	BINDING:

Chain	Residue	Details
A	ARG107
C	ASN139
C	ARG170
C	THR249
D	ARG107
D	ASN139
D	ARG170
D	THR249
E	ARG107
E	ASN139
E	ARG170
A	ASN139
E	THR249
F	ARG107
F	ASN139
F	ARG170
F	THR249
G	ARG107
G	ASN139
G	ARG170
G	THR249
H	ARG107
A	ARG170
H	ASN139
H	ARG170
H	THR249
A	THR249
B	ARG107
B	ASN139
B	ARG170
B	THR249
C	ARG107

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: N-acetylalanine => ECO:0000269\|Ref.4

Chain	Residue	Details
A	ALA2
B	ALA2
C	ALA2
D	ALA2
E	ALA2
F	ALA2
G	ALA2
H	ALA2

site_id	SWS_FT_FI6
Number of Residues	32
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS7
C	LYS58
C	LYS119
C	LYS329
D	LYS7
D	LYS58
D	LYS119
D	LYS329
E	LYS7
E	LYS58
E	LYS119
A	LYS58
E	LYS329
F	LYS7
F	LYS58
F	LYS119
F	LYS329
G	LYS7
G	LYS58
G	LYS119
G	LYS329
H	LYS7
A	LYS119
H	LYS58
H	LYS119
H	LYS329
A	LYS329
B	LYS7
B	LYS58
B	LYS119
B	LYS329
C	LYS7

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19690332

Chain	Residue	Details
A	SER44
B	SER44
C	SER44
D	SER44
E	SER44
F	SER44
G	SER44
H	SER44

site_id	SWS_FT_FI8
Number of Residues	8
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:20068231

Chain	Residue	Details
A	TYR240
B	TYR240
C	TYR240
D	TYR240
E	TYR240
F	TYR240
G	TYR240
H	TYR240

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)