7CTW
Wild-type Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with fragment 820, NADPH, dUMP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0032259 | biological_process | methylation |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005829 | cellular_component | cytosol |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0032259 | biological_process | methylation |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 28 |
| Details | binding site for residue NDP A 701 |
| Chain | Residue |
| A | CYS15 |
| A | THR107 |
| A | SER108 |
| A | SER111 |
| A | LEU127 |
| A | SER128 |
| A | ARG129 |
| A | THR130 |
| A | ASN144 |
| A | ILE164 |
| A | GLY166 |
| A | ALA16 |
| A | SER167 |
| A | VAL168 |
| A | VAL169 |
| A | GLU172 |
| A | VAL195 |
| A | GEX702 |
| A | HOH804 |
| A | HOH814 |
| A | HOH820 |
| A | LEU40 |
| A | GLY41 |
| A | GLY44 |
| A | VAL45 |
| A | LEU46 |
| A | GLY105 |
| A | ARG106 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | binding site for residue GEX A 702 |
| Chain | Residue |
| A | LEU46 |
| A | ASP54 |
| A | MET55 |
| A | PHE58 |
| A | ILE112 |
| A | ILE164 |
| A | NDP701 |
| A | HOH812 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 A 703 |
| Chain | Residue |
| A | ARG510 |
| A | SER511 |
| B | ARG470 |
| B | ARG471 |
| site_id | AC4 |
| Number of Residues | 24 |
| Details | binding site for residue NDP B 701 |
| Chain | Residue |
| B | CYS15 |
| B | ALA16 |
| B | LEU40 |
| B | GLY41 |
| B | GLY44 |
| B | VAL45 |
| B | GLY105 |
| B | ARG106 |
| B | THR107 |
| B | SER108 |
| B | SER111 |
| B | LEU127 |
| B | SER128 |
| B | ARG129 |
| B | THR130 |
| B | ASN144 |
| B | LYS145 |
| B | ILE164 |
| B | GLY165 |
| B | GLY166 |
| B | SER167 |
| B | VAL168 |
| B | VAL169 |
| B | GLU172 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 24 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GLGnkgvLPWkcnslDmkyFcavT |
| Chain | Residue | Details |
| A | GLY39-THR62 |
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriLlcaWNvkdldqma.....LpPCHilcQFyV |
| Chain | Residue | Details |
| A | ARG470-VAL498 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000250 |
| Chain | Residue | Details |
| A | CYS490 | |
| B | CYS490 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 32 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | ARG106 | |
| A | SER108 | |
| A | SER128 | |
| A | ASN144 | |
| A | ILE164 | |
| A | GLY165 | |
| A | TYR170 | |
| A | ARG345 | |
| A | HIS491 | |
| A | GLN509 | |
| A | ASN521 | |
| A | HIS551 | |
| B | ILE14 | |
| B | ALA16 | |
| B | GLY39 | |
| B | ASP54 | |
| B | ARG106 | |
| B | SER108 | |
| B | SER128 | |
| B | ASN144 | |
| B | ILE164 | |
| B | GLY165 | |
| B | TYR170 | |
| B | ARG345 | |
| B | HIS491 | |
| B | GLN509 | |
| B | ASN521 | |
| B | HIS551 | |
| A | ILE14 | |
| A | ALA16 | |
| A | GLY39 | |
| A | ASP54 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| B | VAL31 | |
| B | THR185 | |
| A | VAL31 | |
| A | THR185 |
