7C7A

Cryo-EM structure of yeast Ribonuclease MRP with substrate ITS1

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000171	molecular_function	ribonuclease MRP activity
B	0000172	cellular_component	ribonuclease MRP complex
B	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
B	0000460	biological_process	maturation of 5.8S rRNA
B	0001682	biological_process	tRNA 5'-leader removal
B	0003723	molecular_function	RNA binding
B	0004526	molecular_function	ribonuclease P activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005655	cellular_component	nucleolar ribonuclease P complex
B	0005697	cellular_component	telomerase holoenzyme complex
B	0005737	cellular_component	cytoplasm
B	0006364	biological_process	rRNA processing
B	0008033	biological_process	tRNA processing
B	0016787	molecular_function	hydrolase activity
B	0034965	biological_process	intronic box C/D snoRNA processing
C	0000171	molecular_function	ribonuclease MRP activity
C	0000172	cellular_component	ribonuclease MRP complex
C	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
C	0000460	biological_process	maturation of 5.8S rRNA
C	0001682	biological_process	tRNA 5'-leader removal
C	0004526	molecular_function	ribonuclease P activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005655	cellular_component	nucleolar ribonuclease P complex
C	0005829	cellular_component	cytosol
C	0006364	biological_process	rRNA processing
C	0008033	biological_process	tRNA processing
C	0034965	biological_process	intronic box C/D snoRNA processing
D	0000171	molecular_function	ribonuclease MRP activity
D	0000172	cellular_component	ribonuclease MRP complex
D	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
D	0000460	biological_process	maturation of 5.8S rRNA
D	0001682	biological_process	tRNA 5'-leader removal
D	0003723	molecular_function	RNA binding
D	0004526	molecular_function	ribonuclease P activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005655	cellular_component	nucleolar ribonuclease P complex
D	0006364	biological_process	rRNA processing
D	0006396	biological_process	RNA processing
D	0008033	biological_process	tRNA processing
D	0030677	cellular_component	ribonuclease P complex
D	0033204	molecular_function	ribonuclease P RNA binding
D	0034470	biological_process	obsolete ncRNA processing
D	0034965	biological_process	intronic box C/D snoRNA processing
D	0042134	molecular_function	rRNA primary transcript binding
E	0000171	molecular_function	ribonuclease MRP activity
E	0000172	cellular_component	ribonuclease MRP complex
E	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
E	0000460	biological_process	maturation of 5.8S rRNA
E	0001682	biological_process	tRNA 5'-leader removal
E	0003723	molecular_function	RNA binding
E	0004526	molecular_function	ribonuclease P activity
E	0005634	cellular_component	nucleus
E	0005655	cellular_component	nucleolar ribonuclease P complex
E	0005737	cellular_component	cytoplasm
E	0006364	biological_process	rRNA processing
E	0008033	biological_process	tRNA processing
E	0016787	molecular_function	hydrolase activity
E	0030677	cellular_component	ribonuclease P complex
E	0033204	molecular_function	ribonuclease P RNA binding
E	0034965	biological_process	intronic box C/D snoRNA processing
E	1902555	cellular_component	endoribonuclease complex
E	1990904	cellular_component	ribonucleoprotein complex
F	0000171	molecular_function	ribonuclease MRP activity
F	0000172	cellular_component	ribonuclease MRP complex
F	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
F	0000460	biological_process	maturation of 5.8S rRNA
F	0001682	biological_process	tRNA 5'-leader removal
F	0003676	molecular_function	nucleic acid binding
F	0003723	molecular_function	RNA binding
F	0004526	molecular_function	ribonuclease P activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005655	cellular_component	nucleolar ribonuclease P complex
F	0005697	cellular_component	telomerase holoenzyme complex
F	0005829	cellular_component	cytosol
F	0006364	biological_process	rRNA processing
F	0008033	biological_process	tRNA processing
F	0016787	molecular_function	hydrolase activity
F	0034965	biological_process	intronic box C/D snoRNA processing
F	0042134	molecular_function	rRNA primary transcript binding
G	0000171	molecular_function	ribonuclease MRP activity
G	0000172	cellular_component	ribonuclease MRP complex
G	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
G	0000460	biological_process	maturation of 5.8S rRNA
G	0001682	biological_process	tRNA 5'-leader removal
G	0003676	molecular_function	nucleic acid binding
G	0003723	molecular_function	RNA binding
G	0004526	molecular_function	ribonuclease P activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005655	cellular_component	nucleolar ribonuclease P complex
G	0005697	cellular_component	telomerase holoenzyme complex
G	0006364	biological_process	rRNA processing
G	0006396	biological_process	RNA processing
G	0008033	biological_process	tRNA processing
G	0016787	molecular_function	hydrolase activity
G	0034965	biological_process	intronic box C/D snoRNA processing
H	0000171	molecular_function	ribonuclease MRP activity
H	0000172	cellular_component	ribonuclease MRP complex
H	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
H	0000460	biological_process	maturation of 5.8S rRNA
H	0001682	biological_process	tRNA 5'-leader removal
H	0004526	molecular_function	ribonuclease P activity
H	0005634	cellular_component	nucleus
H	0005655	cellular_component	nucleolar ribonuclease P complex
H	0005829	cellular_component	cytosol
H	0006364	biological_process	rRNA processing
H	0008033	biological_process	tRNA processing
H	0016787	molecular_function	hydrolase activity
H	0034965	biological_process	intronic box C/D snoRNA processing
I	0000171	molecular_function	ribonuclease MRP activity
I	0000172	cellular_component	ribonuclease MRP complex
I	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
I	0000460	biological_process	maturation of 5.8S rRNA
I	0001682	biological_process	tRNA 5'-leader removal
I	0003723	molecular_function	RNA binding
I	0004526	molecular_function	ribonuclease P activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005655	cellular_component	nucleolar ribonuclease P complex
I	0005829	cellular_component	cytosol
I	0006364	biological_process	rRNA processing
I	0008033	biological_process	tRNA processing
I	0016787	molecular_function	hydrolase activity
I	0034965	biological_process	intronic box C/D snoRNA processing
J	0000171	molecular_function	ribonuclease MRP activity
J	0000172	cellular_component	ribonuclease MRP complex
J	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
J	0000460	biological_process	maturation of 5.8S rRNA
J	0001682	biological_process	tRNA 5'-leader removal
J	0003723	molecular_function	RNA binding
J	0004526	molecular_function	ribonuclease P activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005655	cellular_component	nucleolar ribonuclease P complex
J	0005829	cellular_component	cytosol
J	0006364	biological_process	rRNA processing
J	0008033	biological_process	tRNA processing
J	0016787	molecular_function	hydrolase activity
J	0034965	biological_process	intronic box C/D snoRNA processing
K	0000172	cellular_component	ribonuclease MRP complex
K	0000460	biological_process	maturation of 5.8S rRNA
K	0003723	molecular_function	RNA binding
K	0004518	molecular_function	nuclease activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005655	cellular_component	nucleolar ribonuclease P complex
K	0006364	biological_process	rRNA processing
K	0006396	biological_process	RNA processing
K	0006397	biological_process	mRNA processing
K	0008033	biological_process	tRNA processing
K	0008270	molecular_function	zinc ion binding
K	0030541	biological_process	plasmid partitioning
K	0034470	biological_process	obsolete ncRNA processing
K	0042134	molecular_function	rRNA primary transcript binding
K	1902555	cellular_component	endoribonuclease complex
K	1990904	cellular_component	ribonucleoprotein complex
L	0000172	cellular_component	ribonuclease MRP complex
L	0000294	biological_process	nuclear-transcribed mRNA catabolic process, RNase MRP-dependent
L	0000460	biological_process	maturation of 5.8S rRNA
L	0000466	biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
L	0005634	cellular_component	nucleus
L	0005730	cellular_component	nucleolus
L	0005737	cellular_component	cytoplasm
L	0006364	biological_process	rRNA processing
L	0016020	cellular_component	membrane
L	0042134	molecular_function	rRNA primary transcript binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue MG A 401

Chain	Residue
A	A86
A	G87
R	A1

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site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 402

Chain	Residue
A	A86
A	A305
A	A306
R	A0
R	A1

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site_id	AC3
Number of Residues	4
Details	binding site for residue ZN K 200

Chain	Residue
K	HIS63
K	CYS64
K	CYS109
K	CYS61

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site_id	AC4
Number of Residues	2
Details	binding site for residue MG R 101

Chain	Residue
A	G87
R	A1

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	22
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
L	TYR86-LEU108

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