Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7BL7

Crystal structure of UMPK from M. tuberculosis in complex with UDP and UTP (P21212 form)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005886	cellular_component	plasma membrane
A	0006221	biological_process	pyrimidine nucleotide biosynthetic process
A	0006225	biological_process	UDP biosynthetic process
A	0009041	molecular_function	UMP/dUMP kinase activity
A	0016301	molecular_function	kinase activity
A	0033862	molecular_function	UMP kinase activity
A	0044210	biological_process	'de novo' CTP biosynthetic process
A	0046940	biological_process	nucleoside monophosphate phosphorylation
B	0000287	molecular_function	magnesium ion binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005886	cellular_component	plasma membrane
B	0006221	biological_process	pyrimidine nucleotide biosynthetic process
B	0006225	biological_process	UDP biosynthetic process
B	0009041	molecular_function	UMP/dUMP kinase activity
B	0016301	molecular_function	kinase activity
B	0033862	molecular_function	UMP kinase activity
B	0044210	biological_process	'de novo' CTP biosynthetic process
B	0046940	biological_process	nucleoside monophosphate phosphorylation
C	0000287	molecular_function	magnesium ion binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005886	cellular_component	plasma membrane
C	0006221	biological_process	pyrimidine nucleotide biosynthetic process
C	0006225	biological_process	UDP biosynthetic process
C	0009041	molecular_function	UMP/dUMP kinase activity
C	0016301	molecular_function	kinase activity
C	0033862	molecular_function	UMP kinase activity
C	0044210	biological_process	'de novo' CTP biosynthetic process
C	0046940	biological_process	nucleoside monophosphate phosphorylation
D	0000287	molecular_function	magnesium ion binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005886	cellular_component	plasma membrane
D	0006221	biological_process	pyrimidine nucleotide biosynthetic process
D	0006225	biological_process	UDP biosynthetic process
D	0009041	molecular_function	UMP/dUMP kinase activity
D	0016301	molecular_function	kinase activity
D	0033862	molecular_function	UMP kinase activity
D	0044210	biological_process	'de novo' CTP biosynthetic process
D	0046940	biological_process	nucleoside monophosphate phosphorylation
E	0000287	molecular_function	magnesium ion binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005886	cellular_component	plasma membrane
E	0006221	biological_process	pyrimidine nucleotide biosynthetic process
E	0006225	biological_process	UDP biosynthetic process
E	0009041	molecular_function	UMP/dUMP kinase activity
E	0016301	molecular_function	kinase activity
E	0033862	molecular_function	UMP kinase activity
E	0044210	biological_process	'de novo' CTP biosynthetic process
E	0046940	biological_process	nucleoside monophosphate phosphorylation
F	0000287	molecular_function	magnesium ion binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005886	cellular_component	plasma membrane
F	0006221	biological_process	pyrimidine nucleotide biosynthetic process
F	0006225	biological_process	UDP biosynthetic process
F	0009041	molecular_function	UMP/dUMP kinase activity
F	0016301	molecular_function	kinase activity
F	0033862	molecular_function	UMP kinase activity
F	0044210	biological_process	'de novo' CTP biosynthetic process
F	0046940	biological_process	nucleoside monophosphate phosphorylation
G	0000287	molecular_function	magnesium ion binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005886	cellular_component	plasma membrane
G	0006221	biological_process	pyrimidine nucleotide biosynthetic process
G	0006225	biological_process	UDP biosynthetic process
G	0009041	molecular_function	UMP/dUMP kinase activity
G	0016301	molecular_function	kinase activity
G	0033862	molecular_function	UMP kinase activity
G	0044210	biological_process	'de novo' CTP biosynthetic process
G	0046940	biological_process	nucleoside monophosphate phosphorylation
H	0000287	molecular_function	magnesium ion binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005886	cellular_component	plasma membrane
H	0006221	biological_process	pyrimidine nucleotide biosynthetic process
H	0006225	biological_process	UDP biosynthetic process
H	0009041	molecular_function	UMP/dUMP kinase activity
H	0016301	molecular_function	kinase activity
H	0033862	molecular_function	UMP kinase activity
H	0044210	biological_process	'de novo' CTP biosynthetic process
H	0046940	biological_process	nucleoside monophosphate phosphorylation
I	0000287	molecular_function	magnesium ion binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0005886	cellular_component	plasma membrane
I	0006221	biological_process	pyrimidine nucleotide biosynthetic process
I	0006225	biological_process	UDP biosynthetic process
I	0009041	molecular_function	UMP/dUMP kinase activity
I	0016301	molecular_function	kinase activity
I	0033862	molecular_function	UMP kinase activity
I	0044210	biological_process	'de novo' CTP biosynthetic process
I	0046940	biological_process	nucleoside monophosphate phosphorylation
J	0000287	molecular_function	magnesium ion binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0005886	cellular_component	plasma membrane
J	0006221	biological_process	pyrimidine nucleotide biosynthetic process
J	0006225	biological_process	UDP biosynthetic process
J	0009041	molecular_function	UMP/dUMP kinase activity
J	0016301	molecular_function	kinase activity
J	0033862	molecular_function	UMP kinase activity
J	0044210	biological_process	'de novo' CTP biosynthetic process
J	0046940	biological_process	nucleoside monophosphate phosphorylation
K	0000287	molecular_function	magnesium ion binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005886	cellular_component	plasma membrane
K	0006221	biological_process	pyrimidine nucleotide biosynthetic process
K	0006225	biological_process	UDP biosynthetic process
K	0009041	molecular_function	UMP/dUMP kinase activity
K	0016301	molecular_function	kinase activity
K	0033862	molecular_function	UMP kinase activity
K	0044210	biological_process	'de novo' CTP biosynthetic process
K	0046940	biological_process	nucleoside monophosphate phosphorylation
L	0000287	molecular_function	magnesium ion binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0005886	cellular_component	plasma membrane
L	0006221	biological_process	pyrimidine nucleotide biosynthetic process
L	0006225	biological_process	UDP biosynthetic process
L	0009041	molecular_function	UMP/dUMP kinase activity
L	0016301	molecular_function	kinase activity
L	0033862	molecular_function	UMP kinase activity
L	0044210	biological_process	'de novo' CTP biosynthetic process
L	0046940	biological_process	nucleoside monophosphate phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue UDP B 301

Chain	Residue
B	LYS36
B	GLY100
B	MET101
B	GLY157
B	MET158
B	LEU160
B	PHE163
B	SER164
B	THR165
B	THR168
B	GLY38
B	GLY39
B	GLY76
B	GLY77
B	GLY78
B	ARG82
B	GLY83
B	ASP97

site_id	AC2
Number of Residues	12
Details	binding site for residue UTP A 301

Chain	Residue
A	ARG123
A	GLY131
A	ALA134
A	GLU135
A	ARG141
A	HIS145
A	LYS148
C	LEU140
C	ARG141
C	ARG144
C	LYS148
C	UTP301

site_id	AC3
Number of Residues	10
Details	binding site for residue UTP F 301

Chain	Residue
D	LEU140
D	ARG141
D	ARG144
D	LYS148
F	ARG123
F	GLY131
F	ALA134
F	GLU135
F	LYS148
F	ARG150

site_id	AC4
Number of Residues	18
Details	binding site for residue UDP E 301

Chain	Residue
E	LYS36
E	GLY38
E	GLY39
E	GLY76
E	GLY77
E	GLY78
E	ARG82
E	GLY83
E	ASP97
E	GLY100
E	MET101
E	GLY157
E	MET158
E	LEU160
E	PRO161
E	PHE163
E	THR165
E	THR168

site_id	AC5
Number of Residues	13
Details	binding site for residue UTP D 301

Chain	Residue
B	LEU138
B	LEU140
B	ARG141
B	ARG144
B	LYS148
D	ARG123
D	GLY131
D	ALA134
D	GLU135
D	ARG141
D	HIS145
D	LYS148
D	ARG150

site_id	AC6
Number of Residues	15
Details	binding site for residue UDP D 302

Chain	Residue
D	LYS36
D	GLY38
D	GLY39
D	GLY76
D	GLY77
D	GLY78
D	ARG82
D	GLY83
D	ASP97
D	GLY100
D	MET101
D	MET158
D	LEU160
D	PHE163
D	THR165

site_id	AC7
Number of Residues	13
Details	binding site for residue UTP C 301

Chain	Residue
A	UTP301
C	ARG123
C	VAL124
C	GLY131
C	GLU135
C	ARG141
C	LYS148
C	ARG150
E	LEU138
E	LEU140
E	ARG141
E	ARG144
E	LYS148

site_id	AC8
Number of Residues	16
Details	binding site for residue UDP C 302

Chain	Residue
C	LYS36
C	GLY38
C	GLY39
C	GLY77
C	GLY78
C	ARG82
C	GLY83
C	ASP97
C	MET101
C	GLY157
C	MET158
C	LEU160
C	PRO161
C	PHE163
C	SER164
C	THR165

site_id	AC9
Number of Residues	13
Details	binding site for residue UTP I 301

Chain	Residue
G	UTP301
I	ARG123
I	VAL124
I	GLY131
I	GLU135
I	ARG141
I	LYS148
I	ARG150
K	LEU138
K	LEU140
K	ARG141
K	ARG144
K	LYS148

site_id	AD1
Number of Residues	15
Details	binding site for residue UDP I 302

Chain	Residue
I	LYS36
I	GLY38
I	GLY39
I	GLY77
I	GLY78
I	ARG82
I	GLY83
I	ASP97
I	GLY100
I	MET101
I	GLY157
I	MET158
I	LEU160
I	PHE163
I	THR165

site_id	AD2
Number of Residues	13
Details	binding site for residue UTP J 301

Chain	Residue
H	LEU140
H	ARG141
H	ARG144
H	LYS148
J	ARG123
J	GLY131
J	GLU135
J	ARG141
J	HIS145
J	LYS148
J	ARG150
L	ARG141
L	UTP301

site_id	AD3
Number of Residues	17
Details	binding site for residue UDP J 302

Chain	Residue
J	LYS36
J	GLY38
J	GLY39
J	GLY77
J	GLY78
J	ARG82
J	GLY83
J	ASP97
J	MET101
J	GLY157
J	MET158
J	GLY159
J	LEU160
J	PHE163
J	SER164
J	THR165
J	THR168

site_id	AD4
Number of Residues	16
Details	binding site for residue UDP K 301

Chain	Residue
K	LYS36
K	GLY38
K	GLY39
K	GLY77
K	GLY78
K	ARG82
K	GLY83
K	ASP97
K	GLY100
K	MET101
K	GLY157
K	MET158
K	LEU160
K	PHE163
K	THR165
K	THR168

site_id	AD5
Number of Residues	12
Details	binding site for residue UTP L 301

Chain	Residue
J	LEU140
J	ARG141
J	ARG144
J	LYS148
J	UTP301
L	ARG123
L	GLY131
L	GLU135
L	ARG141
L	HIS145
L	LYS148
L	ARG150

site_id	AD6
Number of Residues	13
Details	binding site for residue UTP G 301

Chain	Residue
G	ARG123
G	GLY131
G	GLU135
G	ARG141
G	HIS145
G	LYS148
G	ARG150
G	HOH401
I	LEU140
I	ARG141
I	ARG144
I	LYS148
I	UTP301

site_id	AD7
Number of Residues	15
Details	binding site for residue UDP H 301

Chain	Residue
H	LYS36
H	GLY39
H	GLY76
H	GLY77
H	GLY78
H	ARG82
H	GLY83
H	ASP97
H	GLY100
H	MET101
H	GLY157
H	MET158
H	LEU160
H	PHE163
H	THR165

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	96
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_01220

Chain	Residue	Details
B	LYS36
A	GLY77
A	GLY78
A	ARG82
A	ASP97
A	MET158
A	PHE191
A	ASP194
F	LYS36
F	GLY77
F	GLY78
B	GLY77
F	ARG82
F	ASP97
F	MET158
F	PHE191
F	ASP194
E	LYS36
E	GLY77
E	GLY78
E	ARG82
E	ASP97
B	GLY78
E	MET158
E	PHE191
E	ASP194
D	LYS36
D	GLY77
D	GLY78
D	ARG82
D	ASP97
D	MET158
D	PHE191
B	ARG82
D	ASP194
C	LYS36
C	GLY77
C	GLY78
C	ARG82
C	ASP97
C	MET158
C	PHE191
C	ASP194
I	LYS36
B	ASP97
I	GLY77
I	GLY78
I	ARG82
I	ASP97
I	MET158
I	PHE191
I	ASP194
J	LYS36
J	GLY77
J	GLY78
B	MET158
J	ARG82
J	ASP97
J	MET158
J	PHE191
J	ASP194
K	LYS36
K	GLY77
K	GLY78
K	ARG82
K	ASP97
B	PHE191
K	MET158
K	PHE191
K	ASP194
L	LYS36
L	GLY77
L	GLY78
L	ARG82
L	ASP97
L	MET158
L	PHE191
B	ASP194
L	ASP194
G	LYS36
G	GLY77
G	GLY78
G	ARG82
G	ASP97
G	MET158
G	PHE191
G	ASP194
H	LYS36
A	LYS36
H	GLY77
H	GLY78
H	ARG82
H	ASP97
H	MET158
H	PHE191
H	ASP194

site_id	SWS_FT_FI2
Number of Residues	12
Details	MOD_RES: N-acetylthreonine => ECO:0007744\|PubMed:21969609

Chain	Residue	Details
B	THR2
L	THR2
G	THR2
H	THR2
A	THR2
F	THR2
E	THR2
D	THR2
C	THR2
I	THR2
J	THR2
K	THR2

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)