7BC7
Cryo-EM structure of the proton coupled folate transporter at pH 6.0 bound to pemetrexed
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005768 | cellular_component | endosome |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0010008 | cellular_component | endosome membrane |
| A | 0015293 | molecular_function | symporter activity |
| A | 0015350 | molecular_function | methotrexate transmembrane transporter activity |
| A | 0016323 | cellular_component | basolateral plasma membrane |
| A | 0016324 | cellular_component | apical plasma membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0051958 | biological_process | methotrexate transport |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0140211 | molecular_function | folic acid:proton symporter activity |
| A | 1904447 | biological_process | folate import across plasma membrane |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 18 |
| Details | Transmembrane: {"description":"Helical; Name=TM1","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 71 |
| Details | Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 25 |
| Details | Transmembrane: {"description":"Helical; Name=TM2","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 79 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 22 |
| Details | Transmembrane: {"description":"Helical; Name=TM3","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 13 |
| Details | Transmembrane: {"description":"Helical; Name=TM4","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 25 |
| Details | Transmembrane: {"description":"Helical; Name=TM5","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 18 |
| Details | Transmembrane: {"description":"Helical; Name=TM6","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 22 |
| Details | Transmembrane: {"description":"Helical; Name=TM7","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 22 |
| Details | Transmembrane: {"description":"Helical; Name=TM8","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 19 |
| Details | Transmembrane: {"description":"Helical; Name=TM9","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 20 |
| Details | Transmembrane: {"description":"Helical; Name=TM10","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 25 |
| Details | Transmembrane: {"description":"Helical; Name=TM11","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 18 |
| Details | Transmembrane: {"description":"Helical; Name=TM12","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 5 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7BC7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | Binding site: {"description":"reversibly protonated residue during proton transport","evidences":[{"source":"PubMed","id":"34040256","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 1 |
| Details | Binding site: {"description":"reversibly protonated residue during proton transport","evidences":[{"source":"UniProtKB","id":"Q96NT5","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00498","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
