7A4K
tRNA-guanine transglycosylase C158S/C281S/Y330C/H333A mutant in complex with tetramethylene sulfoxide
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0002099 | biological_process | tRNA wobble guanine modification |
A | 0005737 | cellular_component | cytoplasm |
A | 0005829 | cellular_component | cytosol |
A | 0006400 | biological_process | tRNA modification |
A | 0008033 | biological_process | tRNA processing |
A | 0008479 | molecular_function | tRNA-guanosine(34) queuine transglycosylase activity |
A | 0008616 | biological_process | queuosine biosynthetic process |
A | 0016757 | molecular_function | glycosyltransferase activity |
A | 0016763 | molecular_function | pentosyltransferase activity |
A | 0046872 | molecular_function | metal ion binding |
A | 0101030 | biological_process | tRNA-guanine transglycosylation |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1 |
Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492 |
Chain | Residue | Details |
A | ASP102 |
site_id | SWS_FT_FI2 |
Number of Residues | 1 |
Details | ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492 |
Chain | Residue | Details |
A | ASP280 |
site_id | SWS_FT_FI3 |
Number of Residues | 4 |
Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:12949492 |
Chain | Residue | Details |
A | ASP102 | |
A | ASP156 | |
A | GLN203 | |
A | GLY230 |
site_id | SWS_FT_FI4 |
Number of Residues | 4 |
Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:10413112, ECO:0000269|PubMed:11178905, ECO:0000269|PubMed:11921407, ECO:0000269|PubMed:12646024, ECO:0000269|PubMed:12909636, ECO:0000269|PubMed:12949492, ECO:0000269|PubMed:14523925, ECO:0000269|PubMed:19627989, ECO:0000269|PubMed:8654383, ECO:0000269|PubMed:8961936 |
Chain | Residue | Details |
A | CYS318 | |
A | CYS320 | |
A | CYS323 | |
A | HIS349 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 6 |
Details | M-CSA 881 |
Chain | Residue | Details |
A | ASP102 | proton shuttle (general acid/base) |
A | ASP280 | covalent catalysis |
A | CYS318 | metal ligand |
A | CYS320 | metal ligand |
A | CYS323 | metal ligand |
A | HIS349 | metal ligand |