7A3H

NATIVE ENDOGLUCANASE CEL5A CATALYTIC CORE DOMAIN AT 0.95 ANGSTROMS RESOLUTION

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000272	biological_process	polysaccharide catabolic process
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005975	biological_process	carbohydrate metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 304

Chain	Residue
A	HOH414
A	HOH417
A	GLU80
A	LYS83
A	GLU84
A	GLU87
A	LYS152
A	GLU156
A	GLN189

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EOH A 305

Chain	Residue
A	TRP147
A	GLY148
A	ASN188
A	HOH373
A	HOH495
A	HOH569

---

site_id	ACI
Number of Residues	1
Details	CATALYTIC ACID/BASE

Chain	Residue
A	GLU139

---

site_id	NUC
Number of Residues	1
Details	CATALYTIC NUCLEOPHILE

Chain	Residue
A	GLU228

---

Functional Information from PROSITE/UniProt

site_id	PS00659
Number of Residues	10
Details	GLYCOSYL_HYDROL_F5 Glycosyl hydrolases family 5 signature. VIYEIANEPN

Chain	Residue	Details
A	VAL132-ASN141

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"12595701","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9485319","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9718293","evidenceCode":"ECO:0000305"},{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"journal article","publicationDate":"1999","firstPage":"2621","lastPage":"2622","volume":"121","journal":"J. Am. Chem. Soc.","title":"Lateral protonation of a glycosidase inhibitor. Structure of the Bacillus agaradhaerens Cel5A in complex with a cellobiose-derived imidazole at 0.97 A resolution.","authors":["Varrot A.","Schulein M.","Pipelier M.","Vasella A.","Davies G.J."],"citationCrossReferences":[{"database":"DOI","id":"10.1021/ja984238n"}]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"12595701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9485319","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9718293","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"1999","firstPage":"2621","lastPage":"2622","volume":"121","journal":"J. Am. Chem. Soc.","title":"Lateral protonation of a glycosidase inhibitor. Structure of the Bacillus agaradhaerens Cel5A in complex with a cellobiose-derived imidazole at 0.97 A resolution.","authors":["Varrot A.","Schulein M.","Pipelier M.","Vasella A.","Davies G.J."],"citationCrossReferences":[{"database":"DOI","id":"10.1021/ja984238n"}]}},{"source":"PDB","id":"1H11","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QI2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5A3H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6A3H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8A3H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12595701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9485319","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9718293","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1HF6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2A3H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3A3H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12595701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9485319","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9718293","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1HF6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QI0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2A3H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3A3H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12595701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9718293","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"1999","firstPage":"2621","lastPage":"2622","volume":"121","journal":"J. Am. Chem. Soc.","title":"Lateral protonation of a glycosidase inhibitor. Structure of the Bacillus agaradhaerens Cel5A in complex with a cellobiose-derived imidazole at 0.97 A resolution.","authors":["Varrot A.","Schulein M.","Pipelier M.","Vasella A.","Davies G.J."],"citationCrossReferences":[{"database":"DOI","id":"10.1021/ja984238n"}]}},{"source":"PDB","id":"1HF6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3A3H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12595701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9718293","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1HF6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3A3H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12595701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9485319","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9718293","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"1999","firstPage":"2621","lastPage":"2622","volume":"121","journal":"J. Am. Chem. Soc.","title":"Lateral protonation of a glycosidase inhibitor. Structure of the Bacillus agaradhaerens Cel5A in complex with a cellobiose-derived imidazole at 0.97 A resolution.","authors":["Varrot A.","Schulein M.","Pipelier M.","Vasella A.","Davies G.J."],"citationCrossReferences":[{"database":"DOI","id":"10.1021/ja984238n"}]}},{"source":"PDB","id":"1H5V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1HF6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QI0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2A3H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3A3H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8A3H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1bqc

Chain	Residue	Details
A	ASN138
A	GLU228
A	HIS200
A	GLU139
A	TYR202

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1bqc

Chain	Residue	Details
A	GLU139

---