7A3H

NATIVE ENDOGLUCANASE CEL5A CATALYTIC CORE DOMAIN AT 0.95 ANGSTROMS RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-03
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	54.710, 69.570, 77.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 0.950
R-factor	0.11 *
Rwork	0.110
R-free	0.13000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a3h
RMSD bond length	0.014
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	0.970
High resolution limit [Å]	0.950	0.950
Rmerge	0.043	0.300
Number of reflections	170547
<I/σ(I)>	31	3.5
Completeness [%]	97.0	83.8
Redundancy	5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *		Davies, G.J., (1998) Biochemistry, 37, 1926. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.8-1.2 (M)
2	1	reservoir	glycerol	15 (%(v/v))