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6YNH

GAPDH purified from the supernatant of HEK293F cells: crystal form 4 of 4

Functional Information from GO Data
ChainGOidnamespacecontents
B0000226biological_processmicrotubule cytoskeleton organization
B0001819biological_processpositive regulation of cytokine production
B0002376biological_processimmune system process
B0004365molecular_functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
B0005515molecular_functionprotein binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005811cellular_componentlipid droplet
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005886cellular_componentplasma membrane
B0006006biological_processglucose metabolic process
B0006096biological_processglycolytic process
B0006417biological_processregulation of translation
B0006915biological_processapoptotic process
B0008017molecular_functionmicrotubule binding
B0010951biological_processnegative regulation of endopeptidase activity
B0015630cellular_componentmicrotubule cytoskeleton
B0016020cellular_componentmembrane
B0016241biological_processregulation of macroautophagy
B0016491molecular_functionoxidoreductase activity
B0016620molecular_functionoxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B0016740molecular_functiontransferase activity
B0017148biological_processnegative regulation of translation
B0019828molecular_functionaspartic-type endopeptidase inhibitor activity
B0031640biological_processkilling of cells of another organism
B0031965cellular_componentnuclear membrane
B0031982cellular_componentvesicle
B0032481biological_processpositive regulation of type I interferon production
B0035605molecular_functionpeptidyl-cysteine S-nitrosylase activity
B0035606biological_processpeptidyl-cysteine S-trans-nitrosylation
B0042802molecular_functionidentical protein binding
B0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
B0045087biological_processinnate immune response
B0048471cellular_componentperinuclear region of cytoplasm
B0050661molecular_functionNADP binding
B0050821biological_processprotein stabilization
B0050832biological_processdefense response to fungus
B0051287molecular_functionNAD binding
B0051402biological_processneuron apoptotic process
B0051873biological_processkilling by host of symbiont cells
B0061844biological_processantimicrobial humoral immune response mediated by antimicrobial peptide
B0070062cellular_componentextracellular exosome
B0071346biological_processcellular response to type II interferon
B0097452cellular_componentGAIT complex
B0097718molecular_functiondisordered domain specific binding
B1990904cellular_componentribonucleoprotein complex
D0000226biological_processmicrotubule cytoskeleton organization
D0001819biological_processpositive regulation of cytokine production
D0002376biological_processimmune system process
D0004365molecular_functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
D0005515molecular_functionprotein binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005811cellular_componentlipid droplet
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005886cellular_componentplasma membrane
D0006006biological_processglucose metabolic process
D0006096biological_processglycolytic process
D0006417biological_processregulation of translation
D0006915biological_processapoptotic process
D0008017molecular_functionmicrotubule binding
D0010951biological_processnegative regulation of endopeptidase activity
D0015630cellular_componentmicrotubule cytoskeleton
D0016020cellular_componentmembrane
D0016241biological_processregulation of macroautophagy
D0016491molecular_functionoxidoreductase activity
D0016620molecular_functionoxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D0016740molecular_functiontransferase activity
D0017148biological_processnegative regulation of translation
D0019828molecular_functionaspartic-type endopeptidase inhibitor activity
D0031640biological_processkilling of cells of another organism
D0031965cellular_componentnuclear membrane
D0031982cellular_componentvesicle
D0032481biological_processpositive regulation of type I interferon production
D0035605molecular_functionpeptidyl-cysteine S-nitrosylase activity
D0035606biological_processpeptidyl-cysteine S-trans-nitrosylation
D0042802molecular_functionidentical protein binding
D0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
D0045087biological_processinnate immune response
D0048471cellular_componentperinuclear region of cytoplasm
D0050661molecular_functionNADP binding
D0050821biological_processprotein stabilization
D0050832biological_processdefense response to fungus
D0051287molecular_functionNAD binding
D0051402biological_processneuron apoptotic process
D0051873biological_processkilling by host of symbiont cells
D0061844biological_processantimicrobial humoral immune response mediated by antimicrobial peptide
D0070062cellular_componentextracellular exosome
D0071346biological_processcellular response to type II interferon
D0097452cellular_componentGAIT complex
D0097718molecular_functiondisordered domain specific binding
D1990904cellular_componentribonucleoprotein complex
F0000226biological_processmicrotubule cytoskeleton organization
F0001819biological_processpositive regulation of cytokine production
F0002376biological_processimmune system process
F0004365molecular_functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
F0005515molecular_functionprotein binding
F0005634cellular_componentnucleus
F0005737cellular_componentcytoplasm
F0005811cellular_componentlipid droplet
F0005829cellular_componentcytosol
F0005856cellular_componentcytoskeleton
F0005886cellular_componentplasma membrane
F0006006biological_processglucose metabolic process
F0006096biological_processglycolytic process
F0006417biological_processregulation of translation
F0006915biological_processapoptotic process
F0008017molecular_functionmicrotubule binding
F0010951biological_processnegative regulation of endopeptidase activity
F0015630cellular_componentmicrotubule cytoskeleton
F0016020cellular_componentmembrane
F0016241biological_processregulation of macroautophagy
F0016491molecular_functionoxidoreductase activity
F0016620molecular_functionoxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F0016740molecular_functiontransferase activity
F0017148biological_processnegative regulation of translation
F0019828molecular_functionaspartic-type endopeptidase inhibitor activity
F0031640biological_processkilling of cells of another organism
F0031965cellular_componentnuclear membrane
F0031982cellular_componentvesicle
F0032481biological_processpositive regulation of type I interferon production
F0035605molecular_functionpeptidyl-cysteine S-nitrosylase activity
F0035606biological_processpeptidyl-cysteine S-trans-nitrosylation
F0042802molecular_functionidentical protein binding
F0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
F0045087biological_processinnate immune response
F0048471cellular_componentperinuclear region of cytoplasm
F0050661molecular_functionNADP binding
F0050821biological_processprotein stabilization
F0050832biological_processdefense response to fungus
F0051287molecular_functionNAD binding
F0051402biological_processneuron apoptotic process
F0051873biological_processkilling by host of symbiont cells
F0061844biological_processantimicrobial humoral immune response mediated by antimicrobial peptide
F0070062cellular_componentextracellular exosome
F0071346biological_processcellular response to type II interferon
F0097452cellular_componentGAIT complex
F0097718molecular_functiondisordered domain specific binding
F1990904cellular_componentribonucleoprotein complex
G0000226biological_processmicrotubule cytoskeleton organization
G0001819biological_processpositive regulation of cytokine production
G0002376biological_processimmune system process
G0004365molecular_functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
G0005515molecular_functionprotein binding
G0005634cellular_componentnucleus
G0005737cellular_componentcytoplasm
G0005811cellular_componentlipid droplet
G0005829cellular_componentcytosol
G0005856cellular_componentcytoskeleton
G0005886cellular_componentplasma membrane
G0006006biological_processglucose metabolic process
G0006096biological_processglycolytic process
G0006417biological_processregulation of translation
G0006915biological_processapoptotic process
G0008017molecular_functionmicrotubule binding
G0010951biological_processnegative regulation of endopeptidase activity
G0015630cellular_componentmicrotubule cytoskeleton
G0016020cellular_componentmembrane
G0016241biological_processregulation of macroautophagy
G0016491molecular_functionoxidoreductase activity
G0016620molecular_functionoxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G0016740molecular_functiontransferase activity
G0017148biological_processnegative regulation of translation
G0019828molecular_functionaspartic-type endopeptidase inhibitor activity
G0031640biological_processkilling of cells of another organism
G0031965cellular_componentnuclear membrane
G0031982cellular_componentvesicle
G0032481biological_processpositive regulation of type I interferon production
G0035605molecular_functionpeptidyl-cysteine S-nitrosylase activity
G0035606biological_processpeptidyl-cysteine S-trans-nitrosylation
G0042802molecular_functionidentical protein binding
G0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
G0045087biological_processinnate immune response
G0048471cellular_componentperinuclear region of cytoplasm
G0050661molecular_functionNADP binding
G0050821biological_processprotein stabilization
G0050832biological_processdefense response to fungus
G0051287molecular_functionNAD binding
G0051402biological_processneuron apoptotic process
G0051873biological_processkilling by host of symbiont cells
G0061844biological_processantimicrobial humoral immune response mediated by antimicrobial peptide
G0070062cellular_componentextracellular exosome
G0071346biological_processcellular response to type II interferon
G0097452cellular_componentGAIT complex
G0097718molecular_functiondisordered domain specific binding
G1990904cellular_componentribonucleoprotein complex
Functional Information from PDB Data
site_idAC1
Number of Residues4
Detailsbinding site for residue XPE B 3001
ChainResidue
BASP127
BPRO129
BMET130
BASN136

site_idAC2
Number of Residues4
Detailsbinding site for residue XPE D 3001
ChainResidue
DPRO129
DMET130
DPHE131
DASN136

site_idAC3
Number of Residues4
Detailsbinding site for residue XPE F 3001
ChainResidue
FPRO129
FMET130
FASN136
FALA128

site_idAC4
Number of Residues4
Detailsbinding site for residue XPE G 3001
ChainResidue
GPRO129
GMET130
GPHE131
GASN136

Functional Information from PROSITE/UniProt
site_idPS00071
Number of Residues8
DetailsGAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL
ChainResidueDetails
BALA150-LEU157

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsACT_SITE: Nucleophile => ECO:0000269|PubMed:25086035
ChainResidueDetails
BCSU152
DCSU152
FCSU152
GCSU152

site_idSWS_FT_FI2
Number of Residues20
DetailsBINDING: BINDING => ECO:0000269|PubMed:16239728, ECO:0000269|PubMed:16510976
ChainResidueDetails
BARG13
DASN316
FARG13
FASP35
FARG80
FSER122
FASN316
GARG13
GASP35
GARG80
GSER122
BASP35
GASN316
BARG80
BSER122
BASN316
DARG13
DASP35
DARG80
DSER122

site_idSWS_FT_FI3
Number of Residues16
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P22513
ChainResidueDetails
BSER151
FTHR182
FTHR211
FARG234
GSER151
GTHR182
GTHR211
GARG234
BTHR182
BTHR211
BARG234
DSER151
DTHR182
DTHR211
DARG234
FSER151

site_idSWS_FT_FI4
Number of Residues4
DetailsSITE: Activates thiol group during catalysis => ECO:0000305|PubMed:16239728, ECO:0000305|PubMed:16510976
ChainResidueDetails
BHIS179
DHIS179
FHIS179
GHIS179

site_idSWS_FT_FI5
Number of Residues20
DetailsMOD_RES: N6,N6-dimethyllysine => ECO:0000269|PubMed:18183946
ChainResidueDetails
BLYS5
DLYS334
FLYS5
FLYS66
FLYS260
FLYS263
FLYS334
GLYS5
GLYS66
GLYS260
GLYS263
BLYS66
GLYS334
BLYS260
BLYS263
BLYS334
DLYS5
DLYS66
DLYS260
DLYS263

site_idSWS_FT_FI6
Number of Residues28
DetailsMOD_RES: Deamidated asparagine => ECO:0000269|PubMed:18183946
ChainResidueDetails
BASN9
DASN70
DASN149
DASN155
DASN225
DASN316
FASN9
FASN64
FASN70
FASN149
FASN155
BASN64
FASN225
FASN316
GASN9
GASN64
GASN70
GASN149
GASN155
GASN225
GASN316
BASN70
BASN149
BASN155
BASN225
BASN316
DASN9
DASN64

site_idSWS_FT_FI7
Number of Residues4
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455
ChainResidueDetails
BTYR42
DTYR42
FTYR42
GTYR42

site_idSWS_FT_FI8
Number of Residues4
DetailsMOD_RES: Methionine sulfoxide; in vitro => ECO:0000305|PubMed:25086035
ChainResidueDetails
BMET46
DMET46
FMET46
GMET46

site_idSWS_FT_FI9
Number of Residues12
DetailsMOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861
ChainResidueDetails
BLYS61
GLYS61
GLYS219
GLYS254
BLYS219
BLYS254
DLYS61
DLYS219
DLYS254
FLYS61
FLYS219
FLYS254

site_idSWS_FT_FI10
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:20068231
ChainResidueDetails
BTHR75
DTHR75
FTHR75
GTHR75

site_idSWS_FT_FI11
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163
ChainResidueDetails
BSER83
DSER83
FSER83
GSER83

site_idSWS_FT_FI12
Number of Residues8
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:18183946
ChainResidueDetails
BSER122
BSER148
DSER122
DSER148
FSER122
FSER148
GSER122
GSER148

site_idSWS_FT_FI13
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163
ChainResidueDetails
BSER151
DSER151
FSER151
GSER151

site_idSWS_FT_FI14
Number of Residues4
DetailsMOD_RES: S-nitrosocysteine; in reversibly inhibited form => ECO:0000250|UniProtKB:P04797
ChainResidueDetails
BCSU152
DCSU152
FCSU152
GCSU152

site_idSWS_FT_FI15
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163
ChainResidueDetails
BTHR153
DTHR153
FTHR153
GTHR153

site_idSWS_FT_FI16
Number of Residues8
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569
ChainResidueDetails
BTHR177
BTHR182
DTHR177
DTHR182
FTHR177
FTHR182
GTHR177
GTHR182

site_idSWS_FT_FI17
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:24275569
ChainResidueDetails
BTHR184
DTHR184
FTHR184
GTHR184

site_idSWS_FT_FI18
Number of Residues8
DetailsMOD_RES: N6-malonyllysine; alternate => ECO:0000269|PubMed:21908771
ChainResidueDetails
BLYS194
BLYS215
DLYS194
DLYS215
FLYS194
FLYS215
GLYS194
GLYS215

site_idSWS_FT_FI19
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:19690332
ChainResidueDetails
BTHR211
DTHR211
FTHR211
GTHR211

site_idSWS_FT_FI20
Number of Residues4
DetailsMOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861
ChainResidueDetails
BLYS227
DLYS227
FLYS227
GLYS227

site_idSWS_FT_FI21
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:23186163
ChainResidueDetails
BTHR229
DTHR229
FTHR229
GTHR229

site_idSWS_FT_FI22
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946
ChainResidueDetails
BTHR237
DTHR237
FTHR237
GTHR237

site_idSWS_FT_FI23
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569
ChainResidueDetails
BSER241
DSER241
FSER241
GSER241

site_idSWS_FT_FI24
Number of Residues4
DetailsMOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:22771119, ECO:0000269|PubMed:25417112
ChainResidueDetails
BCYS247
DCYS247
FCYS247
GCYS247

site_idSWS_FT_FI25
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:19690332
ChainResidueDetails
BSER312
DSER312
FSER312
GSER312

site_idSWS_FT_FI26
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163
ChainResidueDetails
BSER333
DSER333
FSER333
GSER333

site_idSWS_FT_FI27
Number of Residues8
DetailsCARBOHYD: (Microbial infection) N-beta-linked (GlcNAc) arginine => ECO:0000269|PubMed:28522607
ChainResidueDetails
BARG197
BARG200
DARG197
DARG200
FARG197
FARG200
GARG197
GARG200

site_idSWS_FT_FI28
Number of Residues8
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733
ChainResidueDetails
BLYS186
DLYS186
FLYS186
GLYS186

237735

PDB entries from 2025-06-18

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