6YNH
GAPDH purified from the supernatant of HEK293F cells: crystal form 4 of 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-22 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.022, 111.299, 69.742 |
| Unit cell angles | 90.00, 98.33, 90.00 |
Refinement procedure
| Resolution | 86.100 - 2.621 |
| R-factor | 0.1781 |
| Rwork | 0.176 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ynd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MLPHARE |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.100 | 2.890 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.400 | 2.470 |
| Rmeas | 0.410 | 2.540 |
| Number of reflections | 28210 | 1409 |
| <I/σ(I)> | 9 | 1.5 |
| Completeness [%] | 71.3 | 14 |
| Redundancy | 17.6 | 17.3 |
| CC(1/2) | 0.990 | 0.464 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Condition F9 of the MORPHEUS crystallisation screen, 0.12M MORPHEUS monosaccharides solution, 0.1M MORPHEUS Buffer System 3, 30% v/v MORPHEUS Precipitant Mix 1 |
