6WYY

Crystal structure of Pseudomonas 7A Glutaminase-Asparaginase in complex with L-Glu at pH 6.5

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004067	molecular_function	asparaginase activity
A	0004359	molecular_function	glutaminase activity
A	0006520	biological_process	amino acid metabolic process
A	0006528	biological_process	asparagine metabolic process
A	0016787	molecular_function	hydrolase activity
A	0042597	cellular_component	periplasmic space
A	0050417	molecular_function	glutamin-(asparagin-)ase activity
B	0004067	molecular_function	asparaginase activity
B	0004359	molecular_function	glutaminase activity
B	0006520	biological_process	amino acid metabolic process
B	0006528	biological_process	asparagine metabolic process
B	0016787	molecular_function	hydrolase activity
B	0042597	cellular_component	periplasmic space
B	0050417	molecular_function	glutamin-(asparagin-)ase activity
C	0004067	molecular_function	asparaginase activity
C	0004359	molecular_function	glutaminase activity
C	0006520	biological_process	amino acid metabolic process
C	0006528	biological_process	asparagine metabolic process
C	0016787	molecular_function	hydrolase activity
C	0042597	cellular_component	periplasmic space
C	0050417	molecular_function	glutamin-(asparagin-)ase activity
D	0004067	molecular_function	asparaginase activity
D	0004359	molecular_function	glutaminase activity
D	0006520	biological_process	amino acid metabolic process
D	0006528	biological_process	asparagine metabolic process
D	0016787	molecular_function	hydrolase activity
D	0042597	cellular_component	periplasmic space
D	0050417	molecular_function	glutamin-(asparagin-)ase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	binding site for residue GLU A 400

Chain	Residue
A	GLY19
A	HOH502
A	HOH581
A	HOH695
B	GLU294
A	THR20
A	ALA66
A	SER67
A	GLU68
A	GLY99
A	THR100
A	ASP101
A	SER125

---

site_id	AC2
Number of Residues	8
Details	binding site for residue EDO A 401

Chain	Residue
A	ALA16
A	THR17
A	GLY18
A	ALA22
A	GLY40
A	GLN61
A	GLN64
A	THR97

---

site_id	AC3
Number of Residues	7
Details	binding site for residue EDO A 402

Chain	Residue
A	LYS205
A	TRP335
A	HOH506
A	HOH595
A	HOH716
D	TRP199
D	HOH641

---

site_id	AC4
Number of Residues	13
Details	binding site for residue GLU B 400

Chain	Residue
A	GLU294
B	GLY19
B	THR20
B	ALA66
B	SER67
B	GLU68
B	GLY99
B	THR100
B	ASP101
B	SER125
B	HOH505
B	HOH549
B	HOH648

---

site_id	AC5
Number of Residues	3
Details	binding site for residue GOL B 401

Chain	Residue
B	ARG81
B	GLU84
B	HOH720

---

site_id	AC6
Number of Residues	6
Details	binding site for residue EDO B 402

Chain	Residue
B	ASN292
B	ALA293
B	GLU294
B	GLN295
B	PRO296
B	HOH615

---

site_id	AC7
Number of Residues	13
Details	binding site for residue GLU C 400

Chain	Residue
C	GLY19
C	THR20
C	ALA66
C	SER67
C	GLU68
C	GLY99
C	THR100
C	ASP101
C	SER125
C	HOH608
C	HOH645
D	GLU294
D	HOH516

---

site_id	AC8
Number of Residues	7
Details	binding site for residue EDO C 401

Chain	Residue
C	ALA32
C	THR33
C	TYR34
C	HOH550
C	HOH725
D	ALA293
D	GLU294

---

site_id	AC9
Number of Residues	7
Details	binding site for residue EDO C 402

Chain	Residue
C	ASP92
C	ALA147
C	SER148
C	ASP149
C	ARG153
C	HOH504
C	HOH614

---

site_id	AD1
Number of Residues	12
Details	binding site for residue GLU D 400

Chain	Residue
C	GLU294
D	GLY19
D	THR20
D	ALA66
D	SER67
D	GLU68
D	GLY99
D	THR100
D	ASP101
D	SER125
D	HOH555
D	HOH659

---

site_id	AD2
Number of Residues	8
Details	binding site for residue EDO D 401

Chain	Residue
B	LYS272
D	PRO222
D	LYS247
D	MET321
D	GLN325
D	HOH503
D	HOH660
D	HOH740

---

Functional Information from PROSITE/UniProt

site_id	PS00144
Number of Residues	9
Details	ASN_GLN_ASE_1 Asparaginase / glutaminase active site signature 1. IlATGGTIA

Chain	Residue	Details
A	ILE14-ALA22

---

site_id	PS00917
Number of Residues	11
Details	ASN_GLN_ASE_2 Asparaginase / glutaminase active site signature 2. GiVitHGTDTL

Chain	Residue	Details
A	GLY93-LEU103

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Acyl-ester intermediate => ECO:0000255|PROSITE-ProRule:PRU10099, ECO:0000255|PROSITE-ProRule:PRU10100

Chain	Residue	Details
A	THR20
B	THR20
C	THR20
D	THR20

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	SER67
A	THR100
B	SER67
B	THR100
C	SER67
C	THR100
D	SER67
D	THR100

---