6WYY
Crystal structure of Pseudomonas 7A Glutaminase-Asparaginase in complex with L-Glu at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.542, 130.791, 81.548 |
| Unit cell angles | 90.00, 117.82, 90.00 |
Refinement procedure
| Resolution | 34.760 - 1.350 |
| R-factor | 0.1231 |
| Rwork | 0.123 |
| R-free | 0.14940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pga |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.121 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.800 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.048 | 0.547 |
| Rpim | 0.033 | 0.402 |
| Number of reflections | 319520 | 15558 |
| <I/σ(I)> | 16 | 2 |
| Completeness [%] | 96.9 | 94.5 |
| Redundancy | 2.7 | 2.6 |
| CC(1/2) | 0.973 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 8% w/v Tacsimate, pH 6.5, 17% w/v PEG3350 |
