6WVN

Crystal Structure of Nsp16-Nsp10 from SARS-CoV-2 in Complex with 7-methyl-GpppA and S-Adenosylmethionine.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004483	molecular_function	methyltransferase cap1 activity
B	0003723	molecular_function	RNA binding
B	0008270	molecular_function	zinc ion binding
B	0019079	biological_process	viral genome replication

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue CL A 7101

Chain	Residue
A	ARG7053
A	ARG7077

---

site_id	AC2
Number of Residues	5
Details	binding site for residue CL A 7102

Chain	Residue
A	PRO7034
A	GLN7036
A	ADE7115
A	HOH7396
A	HOH7440

---

site_id	AC3
Number of Residues	1
Details	binding site for residue CL A 7103

Chain	Residue
A	GLU6945

---

site_id	AC4
Number of Residues	2
Details	binding site for residue CL A 7104

Chain	Residue
A	HOH7382
A	GLY6946

---

site_id	AC5
Number of Residues	6
Details	binding site for residue CL A 7105

Chain	Residue
A	PRO6810
A	ASN6811
A	LEU6812
B	THR4300
B	HOH4503
B	HOH4517

---

site_id	AC6
Number of Residues	2
Details	binding site for residue CL A 7106

Chain	Residue
A	ALA6832
A	LEU6834

---

site_id	AC7
Number of Residues	1
Details	binding site for residue CL A 7107

Chain	Residue
A	ILE6910

---

site_id	AC8
Number of Residues	2
Details	binding site for residue CL A 7108

Chain	Residue
A	LYS6939
A	ASN7083

---

site_id	AC9
Number of Residues	1
Details	binding site for residue CL A 7110

Chain	Residue
A	ASP6900

---

site_id	AD1
Number of Residues	1
Details	binding site for residue CL A 7111

Chain	Residue
A	ARG6817

---

site_id	AD2
Number of Residues	21
Details	binding site for residue SAM A 7112

Chain	Residue
A	ASN6841
A	TYR6845
A	GLY6869
A	GLY6871
A	PRO6878
A	GLY6879
A	ASP6897
A	LEU6898
A	ASN6899
A	ASP6912
A	CYS6913
A	ASP6928
A	MET6929
A	TYR6930
A	GTA7113
A	SO47117
A	HOH7236
A	HOH7254
A	HOH7269
A	HOH7314
A	HOH7401

---

site_id	AD3
Number of Residues	25
Details	binding site for residue GTA A 7113

Chain	Residue
A	CYS6823
A	ASP6824
A	LEU6825
A	TYR6828
A	LYS6844
A	TYR6930
A	LYS6935
A	LYS6968
A	THR6970
A	GLU6971
A	HIS6972
A	SER6999
A	SER7000
A	GLU7001
A	SAM7112
A	SO47117
A	HOH7219
A	HOH7231
A	HOH7239
A	HOH7241
A	HOH7292
A	HOH7332
A	HOH7336
A	HOH7360
A	HOH7407

---

site_id	AD4
Number of Residues	9
Details	binding site for residue MGP A 7114

Chain	Residue
A	LEU6855
A	THR6856
A	TRP6987
A	SER7074
A	HOH7243
A	HOH7310
A	HOH7377
A	HOH7389
B	THR4302

---

site_id	AD5
Number of Residues	4
Details	binding site for residue ADE A 7115

Chain	Residue
A	TRP6803
A	GLN6826
A	CL7102
A	HOH7392

---

site_id	AD6
Number of Residues	2
Details	binding site for residue ADE A 7116

Chain	Residue
A	TYR7020
A	HOH7250

---

site_id	AD7
Number of Residues	7
Details	binding site for residue SO4 A 7117

Chain	Residue
A	ASN6841
A	SAM7112
A	GTA7113
A	HOH7219
A	HOH7227
A	HOH7229
A	HOH7264

---

site_id	AD8
Number of Residues	7
Details	binding site for residue SO4 A 7118

Chain	Residue
A	MET6839
A	MET6840
A	LYS6874
A	HOH7205
A	HOH7255
A	HOH7304
A	HOH7356

---

site_id	AD9
Number of Residues	5
Details	binding site for residue SO4 A 7119

Chain	Residue
A	THR6918
A	LYS6958
A	HOH7388
A	HOH7402
A	HIS6917

---

site_id	AE1
Number of Residues	6
Details	binding site for residue SO4 A 7120

Chain	Residue
A	ARG6864
A	ASN6920
A	LYS6921
A	ARG7081
A	GLU7082
A	ASN7084

---

site_id	AE2
Number of Residues	5
Details	binding site for residue SO4 A 7121

Chain	Residue
A	LYS6836
A	GLY6837
A	HOH7242
A	HOH7393
B	HOH4505

---

site_id	AE3
Number of Residues	2
Details	binding site for residue SO4 A 7122

Chain	Residue
A	ASP6830
A	SER6831

---

site_id	AE4
Number of Residues	2
Details	binding site for residue SO4 A 7123

Chain	Residue
A	LYS7075
A	ARG7077

---

site_id	AE5
Number of Residues	6
Details	binding site for residue SO4 A 7124

Chain	Residue
A	HIS6984
A	GLY7011
A	PRO7013
A	HOH7207
A	HOH7230
A	HOH7368

---

site_id	AE6
Number of Residues	4
Details	binding site for residue ZN B 4401

Chain	Residue
B	CYS4327
B	CYS4330
B	HIS4336
B	CYS4343

---

site_id	AE7
Number of Residues	4
Details	binding site for residue ZN B 4402

Chain	Residue
B	CYS4370
B	CYS4373
B	CYS4381
B	CYS4383

---

site_id	AE8
Number of Residues	3
Details	binding site for residue CL B 4403

Chain	Residue
B	THR4309
B	VAL4310
B	HOH4527

---

site_id	AE9
Number of Residues	1
Details	binding site for residue CL B 4404

Chain	Residue
A	LYS6836

---

site_id	AF1
Number of Residues	3
Details	binding site for residue CL B 4405

Chain	Residue
B	ASP4335
B	HIS4336
B	GLY4341

---

site_id	AF2
Number of Residues	7
Details	binding site for residue SO4 B 4406

Chain	Residue
A	LYS6836
A	GLY6837
A	HOH7272
B	LYS4296
B	ASN4358
B	HOH4505
B	HOH4524

---

site_id	AF3
Number of Residues	5
Details	binding site for residue SO4 B 4407

Chain	Residue
B	THR4300
B	THR4302
B	HOH4513
B	HOH4530
B	HOH4536

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	294
Details	Domain: {"description":"Nidovirus-type SAM-dependent 2'-O-MTase","evidences":[{"source":"PROSITE-ProRule","id":"PRU01300","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	24
Details	Repeat: {"description":"LRR 9","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01300","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01297","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---