6WEP
Crystal structure of TS-DHFR from Cryptosporidium hominis with Apo-TS site
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0032259 | biological_process | methylation |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005829 | cellular_component | cytosol |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0032259 | biological_process | methylation |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | binding site for residue NDP A 601 |
| Chain | Residue |
| A | VAL10 |
| A | THR58 |
| A | SER61 |
| A | ILE75 |
| A | SER76 |
| A | SER77 |
| A | SER78 |
| A | ARG92 |
| A | GLY114 |
| A | GLY115 |
| A | GLU116 |
| A | ALA11 |
| A | SER117 |
| A | THR145 |
| A | MTX602 |
| A | HOH703 |
| A | ILE19 |
| A | GLY23 |
| A | GLN24 |
| A | LEU25 |
| A | GLY55 |
| A | ARG56 |
| A | LYS57 |
| site_id | AC2 |
| Number of Residues | 17 |
| Details | binding site for residue MTX A 602 |
| Chain | Residue |
| A | VAL9 |
| A | VAL10 |
| A | ALA11 |
| A | LEU25 |
| A | ASP32 |
| A | LEU33 |
| A | PHE36 |
| A | SER37 |
| A | THR58 |
| A | ILE62 |
| A | LEU67 |
| A | ARG70 |
| A | CYS113 |
| A | TYR119 |
| A | THR134 |
| A | NDP601 |
| B | ARG65 |
| site_id | AC3 |
| Number of Residues | 23 |
| Details | binding site for residue NDP B 601 |
| Chain | Residue |
| B | VAL10 |
| B | ALA11 |
| B | ILE19 |
| B | GLY23 |
| B | GLN24 |
| B | LEU25 |
| B | GLY55 |
| B | ARG56 |
| B | LYS57 |
| B | THR58 |
| B | SER61 |
| B | ILE75 |
| B | SER76 |
| B | SER77 |
| B | ARG92 |
| B | GLY114 |
| B | GLY115 |
| B | GLU116 |
| B | SER117 |
| B | ILE118 |
| B | ASP121 |
| B | THR145 |
| B | MTX602 |
| site_id | AC4 |
| Number of Residues | 19 |
| Details | binding site for residue MTX B 602 |
| Chain | Residue |
| A | ARG65 |
| B | VAL9 |
| B | VAL10 |
| B | ALA11 |
| B | LEU25 |
| B | ASP32 |
| B | LEU33 |
| B | LYS34 |
| B | PHE36 |
| B | SER37 |
| B | THR58 |
| B | ILE62 |
| B | LEU67 |
| B | ARG70 |
| B | CYS113 |
| B | TYR119 |
| B | THR134 |
| B | NDP601 |
| B | HOH706 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGingqLPWsise.DlkfFskiT |
| Chain | Residue | Details |
| A | GLY18-THR40 |
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RrhIltaWNpsalsqma.....LpPCHvlsQYyV |
| Chain | Residue | Details |
| A | ARG382-VAL410 |
