Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6VND

Quaternary Complex of human dihydroorotate dehydrogenase (DHODH) with flavin mononucleotide (FMN), orotic acid and AG-636

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004151	molecular_function	dihydroorotase activity
A	0004152	molecular_function	dihydroorotate dehydrogenase activity
A	0005515	molecular_function	protein binding
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005829	cellular_component	cytosol
A	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
A	0006221	biological_process	pyrimidine nucleotide biosynthetic process
A	0006225	biological_process	UDP biosynthetic process
A	0009220	biological_process	pyrimidine ribonucleotide biosynthetic process
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0044205	biological_process	'de novo' UMP biosynthetic process
A	0106430	molecular_function	dihydroorotate dehydrogenase (quinone) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	binding site for residue R4P A 401

Chain	Residue
A	TYR37
A	LEU66
A	LEU67
A	ARG135
A	TYR355
A	THR359
A	PRO363
A	DDQ404
A	HOH573
A	LEU41
A	MET42
A	GLN46
A	PRO51
A	ALA54
A	HIS55
A	ALA58
A	THR62

site_id	AC2
Number of Residues	11
Details	binding site for residue DET A 402

Chain	Residue
A	ARG56
A	LYS99
A	HIS100
A	PRO124
A	GLN125
A	GLU126
A	ASN149
A	SER150
A	HIS151
A	NA407
A	HOH613

site_id	AC3
Number of Residues	14
Details	binding site for residue DET A 403

Chain	Residue
A	GLU138
A	ASP139
A	GLN140
A	PRO289
A	LYS306
A	PRO307
A	ASP310
A	GLN314
A	ARG317
A	ASP392
A	HOH528
A	HOH542
A	HOH545
A	HOH611

site_id	AC4
Number of Residues	5
Details	binding site for residue DDQ A 404

Chain	Residue
A	LEU57
A	PHE61
A	LEU67
A	PRO68
A	R4P401

site_id	AC5
Number of Residues	6
Details	binding site for residue DDQ A 405

Chain	Residue
A	GLN140
A	ARG346
A	TRP361
A	GLN380
A	PHE382
A	ALA391

site_id	AC6
Number of Residues	3
Details	binding site for residue CL A 406

Chain	Residue
A	ARG297
A	SER298
A	HOH521

site_id	AC7
Number of Residues	3
Details	binding site for residue NA A 407

Chain	Residue
A	GLU52
A	ARG56
A	DET402

site_id	AC8
Number of Residues	2
Details	binding site for residue NA A 408

Chain	Residue
A	ARG161
A	GLU265

site_id	AC9
Number of Residues	4
Details	binding site for residue NA A 409

Chain	Residue
A	LYS226
A	ARG230
A	SER269
A	GLU273

site_id	AD1
Number of Residues	26
Details	binding site for residue FMN A 410

Chain	Residue
A	ALA94
A	ALA95
A	GLY96
A	LYS99
A	GLY118
A	SER119
A	ASN144
A	TYR146
A	ASN180
A	ASN211
A	LYS254
A	THR282
A	ASN283
A	THR284
A	SER304
A	GLY305
A	LEU308
A	VAL332
A	GLY333
A	GLY334
A	LEU354
A	TYR355
A	THR356
A	ORO411
A	HOH514
A	HOH539

site_id	AD2
Number of Residues	11
Details	binding site for residue ORO A 411

Chain	Residue
A	ASN283
A	THR284
A	FMN410
A	LYS99
A	ASN144
A	TYR146
A	GLY147
A	PHE148
A	ASN211
A	SER214
A	ASN216

site_id	AD3
Number of Residues	3
Details	binding site for residue ACT A 412

Chain	Residue
A	THR260
A	GLN262
A	HOH634

site_id	AD4
Number of Residues	5
Details	binding site for residue ACT A 413

Chain	Residue
A	ALA162
A	LYS166
A	LYS226
A	GLU265
A	ASP266

site_id	AD5
Number of Residues	2
Details	binding site for residue ACT A 414

Chain	Residue
A	ARG248
A	HOH561

site_id	AD6
Number of Residues	4
Details	binding site for residue GOL A 415

Chain	Residue
A	ASP105
A	TYR108
A	HOH546
A	HOH633

site_id	AD7
Number of Residues	5
Details	binding site for residue GOL A 416

Chain	Residue
A	PRO129
A	ARG130
A	PRO131
A	ARG145
A	GLN238

site_id	AD8
Number of Residues	8
Details	binding site for residue GOL A 417

Chain	Residue
A	GLN164
A	GLN167
A	ALA168
A	THR171
A	LEU204
A	ASP206
A	HOH506
A	HOH525

site_id	AD9
Number of Residues	4
Details	binding site for residue GOL A 418

Chain	Residue
A	ARG56
A	LEU57
A	HOH568
A	HOH640

site_id	AE1
Number of Residues	6
Details	binding site for residue GOL A 419

Chain	Residue
A	TYR320
A	ALA321
A	HIS393
A	HOH505
A	HOH543
A	HOH550

site_id	AE2
Number of Residues	2
Details	binding site for residue GOL A 420

Chain	Residue
A	GLY47
A	LEU48

Functional Information from PROSITE/UniProt

site_id	PS00911
Number of Residues	20
Details	DHODEHASE_1 Dihydroorotate dehydrogenase signature 1. GfveiGSVTpkpQeGNprPR

Chain	Residue	Details
A	GLY113-ARG132

site_id	PS00912
Number of Residues	21
Details	DHODEHASE_2 Dihydroorotate dehydrogenase signature 2. IIGvGGVsSgqdAleKIrAGA

Chain	Residue	Details
A	ILE329-ALA349

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	364
Details	TOPO_DOM: Mitochondrial intermembrane => ECO:0000250

Chain	Residue	Details
A	THR31-ARG395

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
A	SER214

site_id	SWS_FT_FI3
Number of Residues	9
Details	BINDING: BINDING => ECO:0000269\|PubMed:10673429, ECO:0000269\|PubMed:16480261

Chain	Residue	Details
A	ALA95
A	SER119
A	ASN180
A	ASN211
A	LYS254
A	THR282
A	GLY305
A	GLY334
A	TYR355

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	LYS99
A	ASN144
A	ASN283

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 109

Chain	Residue	Details
A	ASN144	electrostatic stabiliser
A	PHE148	activator, electrostatic stabiliser, enhance reactivity, polar/non-polar interaction
A	SER214	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, polar/non-polar interaction, proton acceptor, proton donor, proton relay
A	ASN216	electrostatic stabiliser
A	THR217	activator, electrostatic stabiliser, enhance reactivity, hydrogen bond acceptor
A	LYS254	electrostatic stabiliser, hydrogen bond donor
A	ASN283	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)