6VK8
Crystal Structure of Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex with small organic carboxylate at active center
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004497 | molecular_function | monooxygenase activity |
| A | 0006725 | biological_process | obsolete cellular aromatic compound metabolic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0106317 | molecular_function | obsolete methane monooxygenase NADH activity |
| A | 0106318 | molecular_function | obsolete methane monooxygenase NADPH activity |
| B | 0004497 | molecular_function | monooxygenase activity |
| B | 0006725 | biological_process | obsolete cellular aromatic compound metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016709 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen |
| C | 0004497 | molecular_function | monooxygenase activity |
| C | 0015049 | molecular_function | methane monooxygenase [NAD(P)H] activity |
| C | 0015947 | biological_process | methane metabolic process |
| D | 0004497 | molecular_function | monooxygenase activity |
| D | 0006725 | biological_process | obsolete cellular aromatic compound metabolic process |
| E | 0004497 | molecular_function | monooxygenase activity |
| E | 0006725 | biological_process | obsolete cellular aromatic compound metabolic process |
| E | 0006730 | biological_process | one-carbon metabolic process |
| E | 0016491 | molecular_function | oxidoreductase activity |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0106317 | molecular_function | obsolete methane monooxygenase NADH activity |
| E | 0106318 | molecular_function | obsolete methane monooxygenase NADPH activity |
| F | 0004497 | molecular_function | monooxygenase activity |
| F | 0006725 | biological_process | obsolete cellular aromatic compound metabolic process |
| F | 0016491 | molecular_function | oxidoreductase activity |
| F | 0016709 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen |
| G | 0004497 | molecular_function | monooxygenase activity |
| G | 0015049 | molecular_function | methane monooxygenase [NAD(P)H] activity |
| G | 0015947 | biological_process | methane metabolic process |
| H | 0004497 | molecular_function | monooxygenase activity |
| H | 0006725 | biological_process | obsolete cellular aromatic compound metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue FE A 801 |
| Chain | Residue |
| A | GLU114 |
| A | GLU144 |
| A | HIS147 |
| A | FE802 |
| A | SIN803 |
| A | HOH909 |
| A | HOH919 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue FE A 802 |
| Chain | Residue |
| A | GLU243 |
| A | HIS246 |
| A | FE801 |
| A | SIN803 |
| A | HOH919 |
| A | GLU144 |
| A | GLU209 |
| site_id | AC3 |
| Number of Residues | 11 |
| Details | binding site for residue SIN A 803 |
| Chain | Residue |
| A | LEU110 |
| A | GLU114 |
| A | GLU144 |
| A | PHE188 |
| A | GLU209 |
| A | THR213 |
| A | GLU243 |
| A | FE801 |
| A | FE802 |
| A | HOH909 |
| A | HOH919 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 804 |
| Chain | Residue |
| A | GLN406 |
| A | HOH1087 |
| B | TYR304 |
| B | ASN308 |
| B | HOH519 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 805 |
| Chain | Residue |
| A | ARG98 |
| A | THR102 |
| A | TYR288 |
| A | LEU289 |
| A | GLY293 |
| A | HOH903 |
| A | HOH905 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 806 |
| Chain | Residue |
| A | HOH964 |
| A | HOH1005 |
| A | HOH1081 |
| A | HOH1127 |
| B | TRP46 |
| B | LYS47 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 807 |
| Chain | Residue |
| A | VAL105 |
| A | LEU180 |
| A | GLY357 |
| A | PHE358 |
| A | ALA359 |
| A | EDO808 |
| A | HOH903 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 808 |
| Chain | Residue |
| A | ALA350 |
| A | MET353 |
| A | TRP354 |
| A | EDO807 |
| A | HOH903 |
| A | HOH926 |
| site_id | AC9 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 809 |
| Chain | Residue |
| A | ASN272 |
| A | GLU327 |
| A | SER328 |
| A | PRO329 |
| A | ALA330 |
| A | HOH907 |
| A | HOH1140 |
| C | TYR149 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue EDO B 401 |
| Chain | Residue |
| B | SER310 |
| B | GLU311 |
| B | HOH504 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 402 |
| Chain | Residue |
| A | PHE153 |
| B | GLU51 |
| B | HIS110 |
| B | HOH543 |
| H | LYS97 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 403 |
| Chain | Residue |
| B | ALA257 |
| B | VAL364 |
| B | TRP368 |
| B | ASP371 |
| B | HOH532 |
| B | HOH691 |
| site_id | AD4 |
| Number of Residues | 8 |
| Details | binding site for residue EDO C 201 |
| Chain | Residue |
| C | ARG4 |
| C | ILE12 |
| C | ASP49 |
| C | ILE50 |
| C | ASP51 |
| C | VAL52 |
| C | ASP53 |
| C | HOH340 |
| site_id | AD5 |
| Number of Residues | 3 |
| Details | binding site for residue EDO D 201 |
| Chain | Residue |
| A | TRP341 |
| A | HOH974 |
| D | PHE25 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for residue EDO D 202 |
| Chain | Residue |
| A | ARG77 |
| D | GLU47 |
| D | PHE99 |
| D | ASP103 |
| D | ASN107 |
| site_id | AD7 |
| Number of Residues | 6 |
| Details | binding site for residue EDO D 203 |
| Chain | Residue |
| E | ARG88 |
| B | ARG12 |
| D | LYS44 |
| D | ALA73 |
| D | HOH301 |
| D | HOH314 |
| site_id | AD8 |
| Number of Residues | 7 |
| Details | binding site for residue FE E 801 |
| Chain | Residue |
| E | GLU114 |
| E | GLU144 |
| E | HIS147 |
| E | FE802 |
| E | BEZ803 |
| E | HOH919 |
| E | HOH959 |
| site_id | AD9 |
| Number of Residues | 7 |
| Details | binding site for residue FE E 802 |
| Chain | Residue |
| E | GLU144 |
| E | GLU209 |
| E | GLU243 |
| E | HIS246 |
| E | FE801 |
| E | BEZ803 |
| E | HOH959 |
| site_id | AE1 |
| Number of Residues | 11 |
| Details | binding site for residue BEZ E 803 |
| Chain | Residue |
| E | GLU114 |
| E | GLU144 |
| E | PHE188 |
| E | PHE192 |
| E | GLU209 |
| E | THR213 |
| E | GLU243 |
| E | FE801 |
| E | FE802 |
| E | HOH919 |
| E | HOH959 |
| site_id | AE2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO E 804 |
| Chain | Residue |
| E | ARG489 |
| E | GLY492 |
| E | HOH943 |
| E | HOH1049 |
| E | HOH1094 |
| site_id | AE3 |
| Number of Residues | 6 |
| Details | binding site for residue EDO E 805 |
| Chain | Residue |
| B | ASP367 |
| E | LEU14 |
| E | LYS15 |
| E | VAL16 |
| E | ARG18 |
| F | ARG137 |
| site_id | AE4 |
| Number of Residues | 3 |
| Details | binding site for residue EDO E 806 |
| Chain | Residue |
| E | ASP414 |
| E | PRO520 |
| E | HOH1017 |
| site_id | AE5 |
| Number of Residues | 9 |
| Details | binding site for residue EDO E 807 |
| Chain | Residue |
| E | ASN273 |
| E | GLN456 |
| E | GLU460 |
| E | ARG463 |
| E | TYR464 |
| E | EDO808 |
| E | HOH934 |
| E | HOH1035 |
| G | TYR148 |
| site_id | AE6 |
| Number of Residues | 8 |
| Details | binding site for residue EDO E 808 |
| Chain | Residue |
| E | ARG186 |
| E | ASN203 |
| E | ALA274 |
| E | THR277 |
| E | EDO807 |
| E | HOH932 |
| E | HOH1004 |
| F | GLN77 |
| site_id | AE7 |
| Number of Residues | 4 |
| Details | binding site for residue EDO E 809 |
| Chain | Residue |
| E | ASP451 |
| E | TRP452 |
| E | ARG455 |
| E | HOH1030 |
| site_id | AE8 |
| Number of Residues | 6 |
| Details | binding site for residue EDO F 401 |
| Chain | Residue |
| B | PHE126 |
| B | THR278 |
| F | GLN269 |
| F | PRO281 |
| F | ALA285 |
| F | HOH522 |
| site_id | AE9 |
| Number of Residues | 4 |
| Details | binding site for residue EDO F 402 |
| Chain | Residue |
| D | LYS97 |
| E | PHE79 |
| F | GLU51 |
| F | HOH538 |
| site_id | AF1 |
| Number of Residues | 3 |
| Details | binding site for residue EDO F 403 |
| Chain | Residue |
| F | LYS150 |
| F | TYR151 |
| F | HOH529 |
| site_id | AF2 |
| Number of Residues | 8 |
| Details | binding site for residue EDO G 201 |
| Chain | Residue |
| G | ARG4 |
| G | ILE12 |
| G | ASP49 |
| G | ILE50 |
| G | ASP51 |
| G | VAL52 |
| G | ASP53 |
| G | HOH303 |
| site_id | AF3 |
| Number of Residues | 3 |
| Details | binding site for residue EDO G 202 |
| Chain | Residue |
| G | ASP27 |
| G | THR83 |
| G | HOH334 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000255 |
| Chain | Residue | Details |
| A | CYS151 | |
| E | CYS151 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | GLU114 | |
| E | GLU209 | |
| E | GLU243 | |
| E | HIS246 | |
| A | GLU144 | |
| A | HIS147 | |
| A | GLU209 | |
| A | GLU243 | |
| A | HIS246 | |
| E | GLU114 | |
| E | GLU144 | |
| E | HIS147 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 600 |
| Chain | Residue | Details |
| A | GLU114 | metal ligand |
| A | GLU144 | metal ligand |
| A | HIS147 | metal ligand |
| A | GLU209 | metal ligand |
| A | GLU243 | hydrogen bond donor, metal ligand |
| A | HIS246 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 600 |
| Chain | Residue | Details |
| E | GLU114 | metal ligand |
| E | GLU144 | metal ligand |
| E | HIS147 | metal ligand |
| E | GLU209 | metal ligand |
| E | GLU243 | hydrogen bond donor, metal ligand |
| E | HIS246 | metal ligand |
