6VK8

Crystal Structure of Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex with small organic carboxylate at active center

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	101.866, 105.349, 297.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.990 - 2.030
R-factor	0.1688
Rwork	0.167
R-free	0.20750
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.836
Data reduction software	XDS
Data scaling software	PHENIX
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.870	2.100
High resolution limit [Å]	2.030	2.030
Rmerge	0.137
Rpim		1.105
Number of reflections	203307	19824
<I/σ(I)>	7.9
Completeness [%]	98.5
Redundancy	4.1
CC(1/2)	0.994	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Tacsimate, PEG3350