6VIE

Structure of caspase-1 in complex with gasdermin D

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004197	molecular_function	cysteine-type endopeptidase activity
A	0006508	biological_process	proteolysis
A	0008234	molecular_function	cysteine-type peptidase activity
B	0004197	molecular_function	cysteine-type endopeptidase activity
B	0006508	biological_process	proteolysis
B	0008234	molecular_function	cysteine-type peptidase activity
C	0001786	molecular_function	phosphatidylserine binding
C	0005546	molecular_function	phosphatidylinositol-4,5-bisphosphate binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006954	biological_process	inflammatory response
C	0008289	molecular_function	lipid binding
C	0009306	biological_process	protein secretion
C	0012501	biological_process	programmed cell death
C	0016020	cellular_component	membrane
C	0022829	molecular_function	wide pore channel activity
C	0031966	cellular_component	mitochondrial membrane
C	0032731	biological_process	positive regulation of interleukin-1 beta production
C	0045087	biological_process	innate immune response
C	0046931	biological_process	pore complex assembly
C	0050729	biological_process	positive regulation of inflammatory response
C	0050829	biological_process	defense response to Gram-negative bacterium
C	0050830	biological_process	defense response to Gram-positive bacterium
C	0051260	biological_process	protein homooligomerization
C	0055085	biological_process	transmembrane transport
C	0061702	cellular_component	canonical inflammasome complex
C	0070269	biological_process	pyroptotic inflammatory response
C	0070273	molecular_function	phosphatidylinositol-4-phosphate binding
C	0070300	molecular_function	phosphatidic acid binding
C	0072559	cellular_component	NLRP3 inflammasome complex
C	0141201	biological_process	pyroptotic cell death
C	1901612	molecular_function	cardiolipin binding
D	0001786	molecular_function	phosphatidylserine binding
D	0005546	molecular_function	phosphatidylinositol-4,5-bisphosphate binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006954	biological_process	inflammatory response
D	0008289	molecular_function	lipid binding
D	0009306	biological_process	protein secretion
D	0012501	biological_process	programmed cell death
D	0016020	cellular_component	membrane
D	0022829	molecular_function	wide pore channel activity
D	0031966	cellular_component	mitochondrial membrane
D	0032731	biological_process	positive regulation of interleukin-1 beta production
D	0045087	biological_process	innate immune response
D	0046931	biological_process	pore complex assembly
D	0050729	biological_process	positive regulation of inflammatory response
D	0050829	biological_process	defense response to Gram-negative bacterium
D	0050830	biological_process	defense response to Gram-positive bacterium
D	0051260	biological_process	protein homooligomerization
D	0055085	biological_process	transmembrane transport
D	0061702	cellular_component	canonical inflammasome complex
D	0070269	biological_process	pyroptotic inflammatory response
D	0070273	molecular_function	phosphatidylinositol-4-phosphate binding
D	0070300	molecular_function	phosphatidic acid binding
D	0072559	cellular_component	NLRP3 inflammasome complex
D	0141201	biological_process	pyroptotic cell death
D	1901612	molecular_function	cardiolipin binding

Functional Information from PROSITE/UniProt

site_id	PS00455
Number of Residues	12
Details	AMP_BINDING Putative AMP-binding domain signature. MPTSSGSEGnVK

Chain	Residue	Details
A	MET123-LYS134

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site_id	PS01121
Number of Residues	15
Details	CASPASE_HIS Caspase family histidine active site. HktsdStfLvFMSHG

Chain	Residue	Details
A	HIS224-GLY238

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	46
Details	TRANSMEM: Beta stranded => ECO:0000250|UniProtKB:P57764

Chain	Residue	Details
C	GLN92-SER98
C	LYS104-ALA109
C	GLY181-LEU187
C	CYS192-LYS198
D	GLN92-SER98
D	LYS104-ALA109
D	GLY181-LEU187
D	CYS192-LYS198

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site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Cleavage; by CASP3 or CASP7 => ECO:0000269|PubMed:37327784

Chain	Residue	Details
C	ASP88
D	ASP88

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site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Cleavage; by caspases CASP1, CASP4/CASP11 and CASP8 => ECO:0000269|PubMed:26375259, ECO:0000269|PubMed:30361383, ECO:0000269|PubMed:32553275, ECO:0000269|PubMed:38538834, ECO:0000305|PubMed:30135078

Chain	Residue	Details
C	SER300
D	SER300

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site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Cleavage; by papain => ECO:0000269|PubMed:35794369

Chain	Residue	Details
C	SER334
D	SER334

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site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P57764

Chain	Residue	Details
C	TYR38
D	TYR38

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site_id	SWS_FT_FI6
Number of Residues	18
Details	MOD_RES: S-(2-succinyl)cysteine => ECO:0000269|PubMed:32820063

Chain	Residue	Details
C	CYS39
D	CYS39
D	CYS57
D	CYS77
D	CYS122
D	CYS192
D	PHE289
D	GLN323
D	GLN458
C	CYS57
C	CYS77
C	CYS122
C	CYS192
C	PHE289
C	GLN323
C	GLN458

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site_id	SWS_FT_FI7
Number of Residues	2
Details	LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:38530158, ECO:0000269|PubMed:38538834, ECO:0000269|PubMed:38599239

Chain	Residue	Details
C	CYS192
D	CYS192

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 718

Chain	Residue	Details
B	GLU390	electrostatic stabiliser
A	GLY238	electrostatic stabiliser
A	ALA285	nucleofuge, nucleophile, proton acceptor, proton donor
A	ARG286	electrostatic stabiliser

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