6V9I

cryo-EM structure of Cullin5 bound to RING-box protein 2 (Cul5-Rbx2)

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0000082	biological_process	G1/S transition of mitotic cell cycle
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005262	molecular_function	calcium channel activity
C	0005515	molecular_function	protein binding
C	0005618	cellular_component	cell wall
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0007165	biological_process	signal transduction
C	0010498	biological_process	proteasomal protein catabolic process
C	0016477	biological_process	cell migration
C	0016567	biological_process	protein ubiquitination
C	0019005	cellular_component	SCF ubiquitin ligase complex
C	0030335	biological_process	positive regulation of cell migration
C	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
C	0031461	cellular_component	cullin-RING ubiquitin ligase complex
C	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0038023	molecular_function	signaling receptor activity
C	0038026	biological_process	reelin-mediated signaling pathway
C	0038128	biological_process	ERBB2 signaling pathway
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0051895	biological_process	negative regulation of focal adhesion assembly
C	0070588	biological_process	calcium ion transmembrane transport
C	0070979	biological_process	protein K11-linked ubiquitination
C	0090734	cellular_component	site of DNA damage
C	0097193	biological_process	intrinsic apoptotic signaling pathway
C	0120184	biological_process	negative regulation of focal adhesion disassembly
C	0160072	molecular_function	ubiquitin ligase complex scaffold activity
C	2001222	biological_process	regulation of neuron migration
R	0001837	biological_process	epithelial to mesenchymal transition
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0008270	molecular_function	zinc ion binding
R	0008631	biological_process	intrinsic apoptotic signaling pathway in response to oxidative stress
R	0008637	biological_process	apoptotic mitochondrial changes
R	0010498	biological_process	proteasomal protein catabolic process
R	0016567	biological_process	protein ubiquitination
R	0016740	molecular_function	transferase activity
R	0021799	biological_process	cerebral cortex radially oriented cell migration
R	0021942	biological_process	radial glia guided migration of Purkinje cell
R	0030968	biological_process	endoplasmic reticulum unfolded protein response
R	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
R	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
R	0035556	biological_process	intracellular signal transduction
R	0038026	biological_process	reelin-mediated signaling pathway
R	0038162	biological_process	erythropoietin-mediated signaling pathway
R	0043066	biological_process	negative regulation of apoptotic process
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0045087	biological_process	innate immune response
R	0045116	biological_process	protein neddylation
R	0046314	biological_process	phosphocreatine biosynthetic process
R	0046872	molecular_function	metal ion binding
R	0051607	biological_process	defense response to virus
R	0051894	biological_process	positive regulation of focal adhesion assembly
R	0051895	biological_process	negative regulation of focal adhesion assembly
R	0060396	biological_process	growth hormone receptor signaling pathway
R	0060397	biological_process	growth hormone receptor signaling pathway via JAK-STAT
R	0060400	biological_process	negative regulation of growth hormone receptor signaling pathway
R	0061630	molecular_function	ubiquitin protein ligase activity
R	0061663	molecular_function	NEDD8 ligase activity
R	0070979	biological_process	protein K11-linked ubiquitination
R	0097602	molecular_function	cullin family protein binding
R	0140896	biological_process	cGAS/STING signaling pathway
R	2001222	biological_process	regulation of neuron migration

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN R 201

Chain	Residue
R	CYS50
R	CYS53
R	HIS85
R	CYS88

---

site_id	AC2
Number of Residues	3
Details	binding site for residue ZN R 202

Chain	Residue
R	CYS80
R	HIS82
R	CYS99

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN R 203

Chain	Residue
R	CYS73
R	CYS87
R	CYS61
R	CYS64

---

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKeqIewLIEHkYIrRdesdintFiYmA

Chain	Residue	Details
C	ILE753-ALA780

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	61
Details	Domain: {"description":"Cullin neddylation","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)","evidences":[{"source":"PubMed","id":"18805092","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"33268465","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34518685","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7ONI","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"32513959","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6V9I","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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