6V22

Cryo-EM structure of Ca2+-bound hsSlo1-beta4 channel complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0006811	biological_process	monoatomic ion transport
A	0006813	biological_process	potassium ion transport
A	0016020	cellular_component	membrane
A	0055085	biological_process	transmembrane transport
B	0005216	molecular_function	monoatomic ion channel activity
B	0006811	biological_process	monoatomic ion transport
B	0006813	biological_process	potassium ion transport
B	0016020	cellular_component	membrane
B	0055085	biological_process	transmembrane transport
C	0005216	molecular_function	monoatomic ion channel activity
C	0006811	biological_process	monoatomic ion transport
C	0006813	biological_process	potassium ion transport
C	0016020	cellular_component	membrane
C	0055085	biological_process	transmembrane transport
D	0005216	molecular_function	monoatomic ion channel activity
D	0006811	biological_process	monoatomic ion transport
D	0006813	biological_process	potassium ion transport
D	0016020	cellular_component	membrane
D	0055085	biological_process	transmembrane transport
E	0001508	biological_process	action potential
E	0005513	biological_process	detection of calcium ion
E	0005515	molecular_function	protein binding
E	0005886	cellular_component	plasma membrane
E	0006813	biological_process	potassium ion transport
E	0007268	biological_process	chemical synaptic transmission
E	0008076	cellular_component	voltage-gated potassium channel complex
E	0015269	molecular_function	calcium-activated potassium channel activity
E	0015459	molecular_function	potassium channel regulator activity
E	0016020	cellular_component	membrane
E	0019228	biological_process	neuronal action potential
E	0019229	biological_process	regulation of vasoconstriction
E	0034220	biological_process	monoatomic ion transmembrane transport
E	0045202	cellular_component	synapse
E	0046928	biological_process	regulation of neurotransmitter secretion
E	0071805	biological_process	potassium ion transmembrane transport
E	0099505	biological_process	regulation of presynaptic membrane potential
E	0099508	molecular_function	voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential
F	0001508	biological_process	action potential
F	0005513	biological_process	detection of calcium ion
F	0005515	molecular_function	protein binding
F	0005886	cellular_component	plasma membrane
F	0006813	biological_process	potassium ion transport
F	0007268	biological_process	chemical synaptic transmission
F	0008076	cellular_component	voltage-gated potassium channel complex
F	0015269	molecular_function	calcium-activated potassium channel activity
F	0015459	molecular_function	potassium channel regulator activity
F	0016020	cellular_component	membrane
F	0019228	biological_process	neuronal action potential
F	0019229	biological_process	regulation of vasoconstriction
F	0034220	biological_process	monoatomic ion transmembrane transport
F	0045202	cellular_component	synapse
F	0046928	biological_process	regulation of neurotransmitter secretion
F	0071805	biological_process	potassium ion transmembrane transport
F	0099505	biological_process	regulation of presynaptic membrane potential
F	0099508	molecular_function	voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential
G	0001508	biological_process	action potential
G	0005513	biological_process	detection of calcium ion
G	0005515	molecular_function	protein binding
G	0005886	cellular_component	plasma membrane
G	0006813	biological_process	potassium ion transport
G	0007268	biological_process	chemical synaptic transmission
G	0008076	cellular_component	voltage-gated potassium channel complex
G	0015269	molecular_function	calcium-activated potassium channel activity
G	0015459	molecular_function	potassium channel regulator activity
G	0016020	cellular_component	membrane
G	0019228	biological_process	neuronal action potential
G	0019229	biological_process	regulation of vasoconstriction
G	0034220	biological_process	monoatomic ion transmembrane transport
G	0045202	cellular_component	synapse
G	0046928	biological_process	regulation of neurotransmitter secretion
G	0071805	biological_process	potassium ion transmembrane transport
G	0099505	biological_process	regulation of presynaptic membrane potential
G	0099508	molecular_function	voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential
H	0001508	biological_process	action potential
H	0005513	biological_process	detection of calcium ion
H	0005515	molecular_function	protein binding
H	0005886	cellular_component	plasma membrane
H	0006813	biological_process	potassium ion transport
H	0007268	biological_process	chemical synaptic transmission
H	0008076	cellular_component	voltage-gated potassium channel complex
H	0015269	molecular_function	calcium-activated potassium channel activity
H	0015459	molecular_function	potassium channel regulator activity
H	0016020	cellular_component	membrane
H	0019228	biological_process	neuronal action potential
H	0019229	biological_process	regulation of vasoconstriction
H	0034220	biological_process	monoatomic ion transmembrane transport
H	0045202	cellular_component	synapse
H	0046928	biological_process	regulation of neurotransmitter secretion
H	0071805	biological_process	potassium ion transmembrane transport
H	0099505	biological_process	regulation of presynaptic membrane potential
H	0099508	molecular_function	voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential

Functional Information from PROSITE/UniProt

site_id	PS00092
Number of Residues	7
Details	N6_MTASE N-6 Adenine-specific DNA methylases signature. VITNPPY

Chain	Residue	Details
A	VAL1033-TYR1039

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	156
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
E	MET2001-ARG2019
E	VAL2189-SER2210
F	MET2001-ARG2019
F	VAL2189-SER2210
G	MET2001-ARG2019
G	VAL2189-SER2210
H	MET2001-ARG2019
H	VAL2189-SER2210

---

site_id	SWS_FT_FI2
Number of Residues	80
Details	TRANSMEM: Helical; Name=1 => ECO:0000255

Chain	Residue	Details
E	LEU2020-LEU2040
D	TRP43-ARG113
D	ALA171-LYS174
D	LEU221-LEU235
F	LEU2020-LEU2040
G	LEU2020-LEU2040
H	LEU2020-LEU2040
B	ALA171-LYS174
B	LEU221-LEU235
C	TRP43-ARG113
C	ALA171-LYS174
C	LEU221-LEU235

---

site_id	SWS_FT_FI3
Number of Residues	504
Details	TOPO_DOM: Extracellular => ECO:0000255

Chain	Residue	Details
E	SER2041-GLU2167
F	SER2041-GLU2167
G	SER2041-GLU2167
H	SER2041-GLU2167

---

site_id	SWS_FT_FI4
Number of Residues	80
Details	TRANSMEM: Helical; Name=2 => ECO:0000255

Chain	Residue	Details
E	ILE2168-VAL2188
C	SER196-LEU199
C	GLU257-GLN270
C	TYR294-LEU302
D	SER135-THR149
D	SER196-LEU199
D	GLU257-GLN270
D	TYR294-LEU302
F	ILE2168-VAL2188
G	ILE2168-VAL2188
H	ILE2168-VAL2188
B	SER135-THR149
B	SER196-LEU199
B	GLU257-GLN270
B	TYR294-LEU302
C	SER135-THR149

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	CARBOHYD: N-linked (GlcNAc...) asparagine

Chain	Residue	Details
E	ASN2053
E	ASN2090
F	ASN2053
F	ASN2090
G	ASN2053
G	ASN2090
H	ASN2053
H	ASN2090

---

site_id	SWS_FT_FI6
Number of Residues	80
Details	TRANSMEM: Helical; Name=Segment S3 => ECO:0000255

Chain	Residue	Details
A	LEU175-VAL195
B	LEU175-VAL195
C	LEU175-VAL195
D	LEU175-VAL195

---

site_id	SWS_FT_FI7
Number of Residues	80
Details	TRANSMEM: Helical; Name=Segment S4 => ECO:0000255

Chain	Residue	Details
A	ASN200-ILE220
B	ASN200-ILE220
C	ASN200-ILE220
D	ASN200-ILE220

---

site_id	SWS_FT_FI8
Number of Residues	80
Details	TRANSMEM: Helical; Name=Segment S5 => ECO:0000255

Chain	Residue	Details
A	VAL236-VAL256
B	VAL236-VAL256
C	VAL236-VAL256
D	VAL236-VAL256

---

site_id	SWS_FT_FI9
Number of Residues	88
Details	INTRAMEM: Pore-forming; Name=P region => ECO:0000255

Chain	Residue	Details
A	ALA271-VAL293
B	ALA271-VAL293
C	ALA271-VAL293
D	ALA271-VAL293

---

site_id	SWS_FT_FI10
Number of Residues	80
Details	TRANSMEM: Helical; Name=Segment S6 => ECO:0000255

Chain	Residue	Details
A	PHE303-ILE323
B	PHE303-ILE323
C	PHE303-ILE323
D	PHE303-ILE323

---

site_id	SWS_FT_FI11
Number of Residues	12
Details	BINDING: BINDING => ECO:0000305

Chain	Residue	Details
A	GLU374
D	GLU374
D	GLN397
D	GLU399
A	GLN397
A	GLU399
B	GLU374
B	GLN397
B	GLU399
C	GLU374
C	GLN397
C	GLU399

---

site_id	SWS_FT_FI12
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250|UniProtKB:B7ZC96

Chain	Residue	Details
A	PRO947
C	GLU950
C	ILE953
C	GLU955
D	PRO947
D	GLU950
D	ILE953
D	GLU955
A	GLU950
A	ILE953
A	GLU955
B	PRO947
B	GLU950
B	ILE953
B	GLU955
C	PRO947

---

site_id	SWS_FT_FI13
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q08460

Chain	Residue	Details
A	LYS698
A	LEU905
B	LYS698
B	LEU905
C	LYS698
C	LEU905
D	LYS698
D	LEU905

---

site_id	SWS_FT_FI14
Number of Residues	20
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q08460

Chain	Residue	Details
A	ILE700
B	VAL917
C	ILE700
C	THR713
C	GLY717
C	THR913
C	VAL917
D	ILE700
D	THR713
D	GLY717
D	THR913
A	THR713
D	VAL917
A	GLY717
A	THR913
A	VAL917
B	ILE700
B	THR713
B	GLY717
B	THR913

---

site_id	SWS_FT_FI15
Number of Residues	12
Details	LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:20693285, ECO:0000269|PubMed:22399288

Chain	Residue	Details
A	CYS53
D	CYS53
D	CYS54
D	CYS56
A	CYS54
A	CYS56
B	CYS53
B	CYS54
B	CYS56
C	CYS53
C	CYS54
C	CYS56

---