6UW0

Engineered variant of I-OnuI meganuclease with improved thermostability and fully altered specificity targeting cholera toxin A subunit

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004519	molecular_function	endonuclease activity
B	0004519	molecular_function	endonuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue CA A 401

Chain	Residue
A	ALA21
A	GLU178
A	HOH501
A	HOH503
E	DC14
F	DG16

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site_id	AC2
Number of Residues	5
Details	binding site for residue CA A 402

Chain	Residue
E	DT15
F	DT15
A	GLU22
A	GLY177
A	HOH504

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site_id	AC3
Number of Residues	2
Details	binding site for residue EDO A 403

Chain	Residue
A	SER243
A	ARG246

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site_id	AC4
Number of Residues	7
Details	binding site for residue EDO A 404

Chain	Residue
A	LEU225
A	LYS227
A	GLU236
A	CYS238
E	DC17
F	DA8
F	DG9

---

site_id	AC5
Number of Residues	5
Details	binding site for residue CA B 401

Chain	Residue
B	GLU22
B	GLY177
B	HOH502
C	DT15
D	DT15

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site_id	AC6
Number of Residues	6
Details	binding site for residue CA B 402

Chain	Residue
B	ALA21
B	GLU178
B	HOH503
B	HOH504
C	DC14
D	DG16

---

site_id	AC7
Number of Residues	1
Details	binding site for residue CA D 101

Chain	Residue
D	DC26

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site_id	AC8
Number of Residues	4
Details	binding site for residue EDO F 101

Chain	Residue
E	DG-1
E	DG25
F	DC2
F	DT3

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