6UK3
Crystal Structure of S-adenosyl-L-homocysteine hydrolase from Acanthamoeba castellanii with bound NAD and Adenosine
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004013 | molecular_function | adenosylhomocysteinase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0033353 | biological_process | S-adenosylmethionine cycle |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004013 | molecular_function | adenosylhomocysteinase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0033353 | biological_process | S-adenosylmethionine cycle |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004013 | molecular_function | adenosylhomocysteinase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006730 | biological_process | one-carbon metabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0033353 | biological_process | S-adenosylmethionine cycle |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004013 | molecular_function | adenosylhomocysteinase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0006730 | biological_process | one-carbon metabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0033353 | biological_process | S-adenosylmethionine cycle |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 33 |
| Details | binding site for residue NAD A 501 |
| Chain | Residue |
| A | THR203 |
| A | GLU289 |
| A | VAL290 |
| A | ASP291 |
| A | CYS294 |
| A | THR321 |
| A | THR322 |
| A | GLY323 |
| A | ASN324 |
| A | ILE327 |
| A | ILE345 |
| A | THR204 |
| A | GLY346 |
| A | HIS347 |
| A | ASN393 |
| A | HIS400 |
| A | ADN502 |
| A | HOH687 |
| A | HOH700 |
| A | HOH765 |
| A | HOH894 |
| A | HOH896 |
| A | THR205 |
| A | HOH900 |
| C | GLN458 |
| C | LYS471 |
| C | TYR475 |
| A | ASN237 |
| A | GLY266 |
| A | GLY268 |
| A | ASP269 |
| A | VAL270 |
| A | THR288 |
| site_id | AC2 |
| Number of Residues | 16 |
| Details | binding site for residue ADN A 502 |
| Chain | Residue |
| A | HIS64 |
| A | THR66 |
| A | GLN68 |
| A | THR69 |
| A | ASP141 |
| A | GLU202 |
| A | THR203 |
| A | LYS232 |
| A | ASP236 |
| A | HIS347 |
| A | LEU391 |
| A | THR398 |
| A | HIS400 |
| A | MET405 |
| A | PHE409 |
| A | NAD501 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | binding site for residue FMT A 503 |
| Chain | Residue |
| A | HIS338 |
| A | ASN339 |
| A | GLN374 |
| A | HOH627 |
| A | HOH662 |
| D | ASN32 |
| D | LEU33 |
| D | HIS36 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue K A 504 |
| Chain | Residue |
| A | GLN68 |
| A | THR398 |
| A | GLY399 |
| A | HIS400 |
| A | HOH879 |
| A | HOH934 |
| D | HOH702 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue OXM A 505 |
| Chain | Residue |
| A | LEU147 |
| A | SER168 |
| A | GLU170 |
| A | ARG209 |
| A | PHE348 |
| site_id | AC6 |
| Number of Residues | 33 |
| Details | binding site for residue NAD B 501 |
| Chain | Residue |
| D | GLN458 |
| D | LYS471 |
| D | TYR475 |
| B | THR203 |
| B | THR204 |
| B | THR205 |
| B | ASN237 |
| B | GLY266 |
| B | GLY268 |
| B | ASP269 |
| B | VAL270 |
| B | THR288 |
| B | GLU289 |
| B | VAL290 |
| B | ASP291 |
| B | CYS294 |
| B | THR321 |
| B | THR322 |
| B | GLY323 |
| B | ASN324 |
| B | ILE327 |
| B | ILE345 |
| B | GLY346 |
| B | HIS347 |
| B | ASN393 |
| B | HIS400 |
| B | ADN502 |
| B | HOH716 |
| B | HOH727 |
| B | HOH780 |
| B | HOH825 |
| B | HOH839 |
| B | HOH850 |
| site_id | AC7 |
| Number of Residues | 16 |
| Details | binding site for residue ADN B 502 |
| Chain | Residue |
| B | HIS64 |
| B | THR66 |
| B | GLN68 |
| B | THR69 |
| B | ASP141 |
| B | GLU202 |
| B | THR203 |
| B | LYS232 |
| B | ASP236 |
| B | HIS347 |
| B | LEU391 |
| B | THR398 |
| B | HIS400 |
| B | MET405 |
| B | PHE409 |
| B | NAD501 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue ACT B 503 |
| Chain | Residue |
| B | LEU147 |
| B | SER168 |
| B | ARG209 |
| B | HOH706 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue ACT B 504 |
| Chain | Residue |
| B | SER418 |
| B | GLU422 |
| B | LEU435 |
| B | HOH603 |
| B | HOH613 |
| site_id | AD1 |
| Number of Residues | 1 |
| Details | binding site for residue FMT B 505 |
| Chain | Residue |
| B | HOH793 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue FMT B 506 |
| Chain | Residue |
| B | PHE26 |
| B | HOH695 |
| B | HOH970 |
| C | HOH653 |
| site_id | AD3 |
| Number of Residues | 7 |
| Details | binding site for residue K B 507 |
| Chain | Residue |
| B | GLN68 |
| B | THR398 |
| B | GLY399 |
| B | HIS400 |
| B | HOH844 |
| B | HOH897 |
| C | HOH728 |
| site_id | AD4 |
| Number of Residues | 7 |
| Details | binding site for residue FMT B 508 |
| Chain | Residue |
| B | HIS338 |
| B | ASN339 |
| B | GLN374 |
| B | HOH662 |
| C | ASN32 |
| C | LEU33 |
| C | HIS36 |
| site_id | AD5 |
| Number of Residues | 33 |
| Details | binding site for residue NAD C 501 |
| Chain | Residue |
| A | GLN458 |
| A | LYS471 |
| A | TYR475 |
| C | THR203 |
| C | THR204 |
| C | THR205 |
| C | ASN237 |
| C | GLY266 |
| C | GLY268 |
| C | ASP269 |
| C | VAL270 |
| C | THR288 |
| C | GLU289 |
| C | VAL290 |
| C | ASP291 |
| C | CYS294 |
| C | THR321 |
| C | THR322 |
| C | GLY323 |
| C | ASN324 |
| C | ILE327 |
| C | ILE345 |
| C | GLY346 |
| C | HIS347 |
| C | ASN393 |
| C | HIS400 |
| C | ADN502 |
| C | HOH722 |
| C | HOH727 |
| C | HOH784 |
| C | HOH846 |
| C | HOH882 |
| C | HOH886 |
| site_id | AD6 |
| Number of Residues | 16 |
| Details | binding site for residue ADN C 502 |
| Chain | Residue |
| C | HIS64 |
| C | THR66 |
| C | GLN68 |
| C | THR69 |
| C | ASP141 |
| C | GLU202 |
| C | THR203 |
| C | LYS232 |
| C | ASP236 |
| C | HIS347 |
| C | LEU391 |
| C | THR398 |
| C | HIS400 |
| C | MET405 |
| C | PHE409 |
| C | NAD501 |
| site_id | AD7 |
| Number of Residues | 8 |
| Details | binding site for residue FMT C 503 |
| Chain | Residue |
| B | ASN32 |
| B | LEU33 |
| B | HIS36 |
| C | HIS338 |
| C | ASN339 |
| C | GLN374 |
| C | HOH645 |
| C | HOH783 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue ACT C 504 |
| Chain | Residue |
| C | LEU147 |
| C | VAL174 |
| C | ARG209 |
| C | HOH622 |
| site_id | AD9 |
| Number of Residues | 3 |
| Details | binding site for residue FMT C 505 |
| Chain | Residue |
| C | PHE26 |
| C | HOH610 |
| C | HOH908 |
| site_id | AE1 |
| Number of Residues | 7 |
| Details | binding site for residue K C 506 |
| Chain | Residue |
| B | HOH710 |
| C | GLN68 |
| C | THR398 |
| C | GLY399 |
| C | HIS400 |
| C | HOH853 |
| C | HOH906 |
| site_id | AE2 |
| Number of Residues | 33 |
| Details | binding site for residue NAD D 501 |
| Chain | Residue |
| B | GLN458 |
| B | LYS471 |
| B | TYR475 |
| D | THR203 |
| D | THR204 |
| D | THR205 |
| D | ASN237 |
| D | GLY266 |
| D | GLY268 |
| D | ASP269 |
| D | VAL270 |
| D | THR288 |
| D | GLU289 |
| D | VAL290 |
| D | ASP291 |
| D | CYS294 |
| D | THR321 |
| D | THR322 |
| D | GLY323 |
| D | ASN324 |
| D | ILE327 |
| D | ILE345 |
| D | GLY346 |
| D | HIS347 |
| D | ASN393 |
| D | HIS400 |
| D | ADN502 |
| D | HOH707 |
| D | HOH723 |
| D | HOH758 |
| D | HOH839 |
| D | HOH864 |
| D | HOH893 |
| site_id | AE3 |
| Number of Residues | 16 |
| Details | binding site for residue ADN D 502 |
| Chain | Residue |
| D | HIS64 |
| D | THR66 |
| D | GLN68 |
| D | THR69 |
| D | ASP141 |
| D | GLU202 |
| D | THR203 |
| D | LYS232 |
| D | ASP236 |
| D | HIS347 |
| D | LEU391 |
| D | THR398 |
| D | HIS400 |
| D | MET405 |
| D | PHE409 |
| D | NAD501 |
| site_id | AE4 |
| Number of Residues | 8 |
| Details | binding site for residue FMT D 503 |
| Chain | Residue |
| A | ASN32 |
| A | LEU33 |
| A | HIS36 |
| D | HIS338 |
| D | ASN339 |
| D | GLN374 |
| D | HOH642 |
| D | HOH780 |
| site_id | AE5 |
| Number of Residues | 4 |
| Details | binding site for residue ACT D 504 |
| Chain | Residue |
| D | LEU147 |
| D | ARG209 |
| D | HOH602 |
| D | HOH711 |
| site_id | AE6 |
| Number of Residues | 4 |
| Details | binding site for residue FMT D 505 |
| Chain | Residue |
| D | HIS150 |
| D | TYR154 |
| D | HOH611 |
| D | HOH791 |
| site_id | AE7 |
| Number of Residues | 3 |
| Details | binding site for residue FMT D 506 |
| Chain | Residue |
| D | LYS191 |
| D | PRO194 |
| D | GLU195 |
| site_id | AE8 |
| Number of Residues | 6 |
| Details | binding site for residue ACT D 507 |
| Chain | Residue |
| D | SER418 |
| D | GLU422 |
| D | PRO432 |
| D | LEU435 |
| D | HOH609 |
| D | HOH632 |
| site_id | AE9 |
| Number of Residues | 7 |
| Details | binding site for residue K D 508 |
| Chain | Residue |
| A | HOH698 |
| D | GLN68 |
| D | THR398 |
| D | GLY399 |
| D | HIS400 |
| D | HOH857 |
| D | HOH915 |
Functional Information from PROSITE/UniProt
| site_id | PS00738 |
| Number of Residues | 15 |
| Details | ADOHCYASE_1 S-adenosyl-L-homocysteine hydrolase signature 1. SCNiFSTQDhAAAAI |
| Chain | Residue | Details |
| A | SER87-ILE101 |
| site_id | PS00739 |
| Number of Residues | 17 |
| Details | ADOHCYASE_2 S-adenosyl-L-homocysteine hydrolase signature 2. GKvacVlGYGdVGKGs.A |
| Chain | Residue | Details |
| A | GLY259-ALA275 |
