Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6U8E

Human IMPDH2 treated with ATP, IMP, and NAD+. Filament assembly interface reconstruction.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0003824	molecular_function	catalytic activity
A	0003938	molecular_function	IMP dehydrogenase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005778	cellular_component	peroxisomal membrane
A	0005829	cellular_component	cytosol
A	0006164	biological_process	purine nucleotide biosynthetic process
A	0006177	biological_process	GMP biosynthetic process
A	0006183	biological_process	GTP biosynthetic process
A	0007623	biological_process	circadian rhythm
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0034774	cellular_component	secretory granule lumen
A	0046651	biological_process	lymphocyte proliferation
A	0046872	molecular_function	metal ion binding
A	0070062	cellular_component	extracellular exosome
A	0071353	biological_process	cellular response to interleukin-4
A	0097294	biological_process	'de novo' XMP biosynthetic process
A	1904813	cellular_component	ficolin-1-rich granule lumen
B	0000166	molecular_function	nucleotide binding
B	0003677	molecular_function	DNA binding
B	0003723	molecular_function	RNA binding
B	0003824	molecular_function	catalytic activity
B	0003938	molecular_function	IMP dehydrogenase activity
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005778	cellular_component	peroxisomal membrane
B	0005829	cellular_component	cytosol
B	0006164	biological_process	purine nucleotide biosynthetic process
B	0006177	biological_process	GMP biosynthetic process
B	0006183	biological_process	GTP biosynthetic process
B	0007623	biological_process	circadian rhythm
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0034774	cellular_component	secretory granule lumen
B	0046651	biological_process	lymphocyte proliferation
B	0046872	molecular_function	metal ion binding
B	0070062	cellular_component	extracellular exosome
B	0071353	biological_process	cellular response to interleukin-4
B	0097294	biological_process	'de novo' XMP biosynthetic process
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0000166	molecular_function	nucleotide binding
C	0003677	molecular_function	DNA binding
C	0003723	molecular_function	RNA binding
C	0003824	molecular_function	catalytic activity
C	0003938	molecular_function	IMP dehydrogenase activity
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005778	cellular_component	peroxisomal membrane
C	0005829	cellular_component	cytosol
C	0006164	biological_process	purine nucleotide biosynthetic process
C	0006177	biological_process	GMP biosynthetic process
C	0006183	biological_process	GTP biosynthetic process
C	0007623	biological_process	circadian rhythm
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0034774	cellular_component	secretory granule lumen
C	0046651	biological_process	lymphocyte proliferation
C	0046872	molecular_function	metal ion binding
C	0070062	cellular_component	extracellular exosome
C	0071353	biological_process	cellular response to interleukin-4
C	0097294	biological_process	'de novo' XMP biosynthetic process
C	1904813	cellular_component	ficolin-1-rich granule lumen
D	0000166	molecular_function	nucleotide binding
D	0003677	molecular_function	DNA binding
D	0003723	molecular_function	RNA binding
D	0003824	molecular_function	catalytic activity
D	0003938	molecular_function	IMP dehydrogenase activity
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005778	cellular_component	peroxisomal membrane
D	0005829	cellular_component	cytosol
D	0006164	biological_process	purine nucleotide biosynthetic process
D	0006177	biological_process	GMP biosynthetic process
D	0006183	biological_process	GTP biosynthetic process
D	0007623	biological_process	circadian rhythm
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0034774	cellular_component	secretory granule lumen
D	0046651	biological_process	lymphocyte proliferation
D	0046872	molecular_function	metal ion binding
D	0070062	cellular_component	extracellular exosome
D	0071353	biological_process	cellular response to interleukin-4
D	0097294	biological_process	'de novo' XMP biosynthetic process
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0000166	molecular_function	nucleotide binding
E	0003677	molecular_function	DNA binding
E	0003723	molecular_function	RNA binding
E	0003824	molecular_function	catalytic activity
E	0003938	molecular_function	IMP dehydrogenase activity
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005778	cellular_component	peroxisomal membrane
E	0005829	cellular_component	cytosol
E	0006164	biological_process	purine nucleotide biosynthetic process
E	0006177	biological_process	GMP biosynthetic process
E	0006183	biological_process	GTP biosynthetic process
E	0007623	biological_process	circadian rhythm
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0034774	cellular_component	secretory granule lumen
E	0046651	biological_process	lymphocyte proliferation
E	0046872	molecular_function	metal ion binding
E	0070062	cellular_component	extracellular exosome
E	0071353	biological_process	cellular response to interleukin-4
E	0097294	biological_process	'de novo' XMP biosynthetic process
E	1904813	cellular_component	ficolin-1-rich granule lumen
F	0000166	molecular_function	nucleotide binding
F	0003677	molecular_function	DNA binding
F	0003723	molecular_function	RNA binding
F	0003824	molecular_function	catalytic activity
F	0003938	molecular_function	IMP dehydrogenase activity
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005778	cellular_component	peroxisomal membrane
F	0005829	cellular_component	cytosol
F	0006164	biological_process	purine nucleotide biosynthetic process
F	0006177	biological_process	GMP biosynthetic process
F	0006183	biological_process	GTP biosynthetic process
F	0007623	biological_process	circadian rhythm
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0034774	cellular_component	secretory granule lumen
F	0046651	biological_process	lymphocyte proliferation
F	0046872	molecular_function	metal ion binding
F	0070062	cellular_component	extracellular exosome
F	0071353	biological_process	cellular response to interleukin-4
F	0097294	biological_process	'de novo' XMP biosynthetic process
F	1904813	cellular_component	ficolin-1-rich granule lumen
G	0000166	molecular_function	nucleotide binding
G	0003677	molecular_function	DNA binding
G	0003723	molecular_function	RNA binding
G	0003824	molecular_function	catalytic activity
G	0003938	molecular_function	IMP dehydrogenase activity
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005778	cellular_component	peroxisomal membrane
G	0005829	cellular_component	cytosol
G	0006164	biological_process	purine nucleotide biosynthetic process
G	0006177	biological_process	GMP biosynthetic process
G	0006183	biological_process	GTP biosynthetic process
G	0007623	biological_process	circadian rhythm
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0034774	cellular_component	secretory granule lumen
G	0046651	biological_process	lymphocyte proliferation
G	0046872	molecular_function	metal ion binding
G	0070062	cellular_component	extracellular exosome
G	0071353	biological_process	cellular response to interleukin-4
G	0097294	biological_process	'de novo' XMP biosynthetic process
G	1904813	cellular_component	ficolin-1-rich granule lumen
H	0000166	molecular_function	nucleotide binding
H	0003677	molecular_function	DNA binding
H	0003723	molecular_function	RNA binding
H	0003824	molecular_function	catalytic activity
H	0003938	molecular_function	IMP dehydrogenase activity
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005778	cellular_component	peroxisomal membrane
H	0005829	cellular_component	cytosol
H	0006164	biological_process	purine nucleotide biosynthetic process
H	0006177	biological_process	GMP biosynthetic process
H	0006183	biological_process	GTP biosynthetic process
H	0007623	biological_process	circadian rhythm
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0034774	cellular_component	secretory granule lumen
H	0046651	biological_process	lymphocyte proliferation
H	0046872	molecular_function	metal ion binding
H	0070062	cellular_component	extracellular exosome
H	0071353	biological_process	cellular response to interleukin-4
H	0097294	biological_process	'de novo' XMP biosynthetic process
H	1904813	cellular_component	ficolin-1-rich granule lumen

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue IMP A 601

Chain	Residue
A	SER68
A	MET385
A	GLY387
A	SER388
A	TYR411
A	GLY413
A	MET414
A	GLY415
A	GLN441
A	NAD602
A	ARG322
A	GLY328
A	SER329
A	ILE330
A	CYS331
A	ASP364
A	GLY365
A	GLY366

site_id	AC2
Number of Residues	15
Details	binding site for residue NAD A 602

Chain	Residue
A	HIS253
A	ASP274
A	SER275
A	SER276
A	PHE282
A	ASN303
A	GLY324
A	MET325
A	GLY326
A	CYS331
A	MET414
A	GLY415
A	GLN441
A	IMP601
D	GLN469

site_id	AC3
Number of Residues	18
Details	binding site for residue IMP B 601

Chain	Residue
B	SER68
B	ARG322
B	GLY328
B	SER329
B	ILE330
B	CYS331
B	ASP364
B	GLY365
B	GLY366
B	MET385
B	GLY387
B	SER388
B	TYR411
B	GLY413
B	MET414
B	GLY415
B	GLN441
B	NAD602

site_id	AC4
Number of Residues	15
Details	binding site for residue NAD B 602

Chain	Residue
A	GLN469
B	HIS253
B	ASP274
B	SER275
B	SER276
B	PHE282
B	ASN303
B	GLY324
B	MET325
B	GLY326
B	CYS331
B	MET414
B	GLY415
B	GLN441
B	IMP601

site_id	AC5
Number of Residues	18
Details	binding site for residue IMP C 601

Chain	Residue
C	SER68
C	ARG322
C	GLY328
C	SER329
C	ILE330
C	CYS331
C	ASP364
C	GLY365
C	GLY366
C	MET385
C	GLY387
C	SER388
C	TYR411
C	GLY413
C	MET414
C	GLY415
C	GLN441
C	NAD602

site_id	AC6
Number of Residues	15
Details	binding site for residue NAD C 602

Chain	Residue
B	GLN469
C	HIS253
C	ASP274
C	SER275
C	SER276
C	PHE282
C	ASN303
C	GLY324
C	MET325
C	GLY326
C	CYS331
C	MET414
C	GLY415
C	GLN441
C	IMP601

site_id	AC7
Number of Residues	18
Details	binding site for residue IMP D 601

Chain	Residue
D	SER68
D	ARG322
D	GLY328
D	SER329
D	ILE330
D	CYS331
D	ASP364
D	GLY365
D	GLY366
D	MET385
D	GLY387
D	SER388
D	TYR411
D	GLY413
D	MET414
D	GLY415
D	GLN441
D	NAD602

site_id	AC8
Number of Residues	15
Details	binding site for residue NAD D 602

Chain	Residue
C	GLN469
D	HIS253
D	ASP274
D	SER275
D	SER276
D	PHE282
D	ASN303
D	GLY324
D	MET325
D	GLY326
D	CYS331
D	MET414
D	GLY415
D	GLN441
D	IMP601

site_id	AC9
Number of Residues	18
Details	binding site for residue IMP E 601

Chain	Residue
E	SER68
E	ARG322
E	GLY328
E	SER329
E	ILE330
E	CYS331
E	ASP364
E	GLY365
E	GLY366
E	MET385
E	GLY387
E	SER388
E	TYR411
E	GLY413
E	MET414
E	GLY415
E	GLN441
E	NAD602

site_id	AD1
Number of Residues	15
Details	binding site for residue NAD E 602

Chain	Residue
E	HIS253
E	ASP274
E	SER275
E	SER276
E	PHE282
E	ASN303
E	GLY324
E	MET325
E	GLY326
E	CYS331
E	MET414
E	GLY415
E	GLN441
E	IMP601
H	GLN469

site_id	AD2
Number of Residues	18
Details	binding site for residue IMP F 601

Chain	Residue
F	SER68
F	ARG322
F	GLY328
F	SER329
F	ILE330
F	CYS331
F	ASP364
F	GLY365
F	GLY366
F	MET385
F	GLY387
F	SER388
F	TYR411
F	GLY413
F	MET414
F	GLY415
F	GLN441
F	NAD602

site_id	AD3
Number of Residues	15
Details	binding site for residue NAD F 602

Chain	Residue
E	GLN469
F	HIS253
F	ASP274
F	SER275
F	SER276
F	PHE282
F	ASN303
F	GLY324
F	MET325
F	GLY326
F	CYS331
F	MET414
F	GLY415
F	GLN441
F	IMP601

site_id	AD4
Number of Residues	18
Details	binding site for residue IMP G 601

Chain	Residue
G	SER68
G	ARG322
G	GLY328
G	SER329
G	ILE330
G	CYS331
G	ASP364
G	GLY365
G	GLY366
G	MET385
G	GLY387
G	SER388
G	TYR411
G	GLY413
G	MET414
G	GLY415
G	GLN441
G	NAD602

site_id	AD5
Number of Residues	15
Details	binding site for residue NAD G 602

Chain	Residue
F	GLN469
G	HIS253
G	ASP274
G	SER275
G	SER276
G	PHE282
G	ASN303
G	GLY324
G	MET325
G	GLY326
G	CYS331
G	MET414
G	GLY415
G	GLN441
G	IMP601

site_id	AD6
Number of Residues	18
Details	binding site for residue IMP H 601

Chain	Residue
H	SER68
H	ARG322
H	GLY328
H	SER329
H	ILE330
H	CYS331
H	ASP364
H	GLY365
H	GLY366
H	MET385
H	GLY387
H	SER388
H	TYR411
H	GLY413
H	MET414
H	GLY415
H	GLN441
H	NAD602

site_id	AD7
Number of Residues	15
Details	binding site for residue NAD H 602

Chain	Residue
G	GLN469
H	HIS253
H	ASP274
H	SER275
H	SER276
H	PHE282
H	ASN303
H	GLY324
H	MET325
H	GLY326
H	CYS331
H	MET414
H	GLY415
H	GLN441
H	IMP601

Functional Information from PROSITE/UniProt

site_id	PS00487
Number of Residues	13
Details	IMP_DH_GMP_RED IMP dehydrogenase / GMP reductase signature. LRVGMGsGSICiT

Chain	Residue	Details
A	LEU321-THR333

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Thioimidate intermediate => ECO:0000255\|HAMAP-Rule:MF_03156, ECO:0000269\|PubMed:10097070

Chain	Residue	Details
A	CYS331
B	CYS331
C	CYS331
D	CYS331
E	CYS331
F	CYS331
G	CYS331
H	CYS331

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: Proton acceptor => ECO:0000255\|HAMAP-Rule:MF_03156

Chain	Residue	Details
B	ARG429
C	ARG429
D	ARG429
E	ARG429
F	ARG429
G	ARG429
H	ARG429
A	ARG429

site_id	SWS_FT_FI3
Number of Residues	24
Details	BINDING:

Chain	Residue	Details
C	GLY387
D	ASP274
D	ASP364
D	GLY387
E	ASP274
E	ASP364
E	GLY387
F	ASP274
F	ASP364
F	GLY387
G	ASP274
G	ASP364
G	GLY387
H	ASP274
H	ASP364
H	GLY387
A	ASP364
A	GLY387
B	ASP274
B	ASP364
B	GLY387
C	ASP274
C	ASP364
A	ASP274

site_id	SWS_FT_FI4
Number of Residues	56
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_03156

Chain	Residue	Details
B	SER329
B	TYR411
B	GLN441
B	GLU500
B	GLY501
B	GLY502
C	GLY324
C	SER329
C	TYR411
C	GLN441
C	GLU500
C	GLY501
C	GLY502
D	GLY324
D	SER329
D	TYR411
D	GLN441
D	GLU500
D	GLY501
D	GLY502
E	GLY324
E	SER329
E	TYR411
E	GLN441
E	GLU500
E	GLY501
E	GLY502
F	GLY324
F	SER329
F	TYR411
F	GLN441
F	GLU500
F	GLY501
F	GLY502
G	GLY324
G	SER329
G	TYR411
G	GLN441
G	GLU500
G	GLY501
G	GLY502
H	GLY324
H	SER329
H	TYR411
H	GLN441
H	GLU500
H	GLY501
H	GLY502
A	GLN441
A	GLU500
A	GLY501
A	GLY502
B	GLY324
A	GLY324
A	SER329
A	TYR411

site_id	SWS_FT_FI5
Number of Residues	24
Details	BINDING: in other chain => ECO:0000255\|HAMAP-Rule:MF_03156, ECO:0000269\|Ref.28

Chain	Residue	Details
G	GLY326
G	GLY328
B	GLY326
B	GLY328
B	CYS331
C	GLY326
C	GLY328
C	CYS331
D	GLY326
D	GLY328
D	CYS331
E	GLY326
E	GLY328
E	CYS331
F	GLY326
F	GLY328
F	CYS331
G	CYS331
H	GLY326
H	GLY328
H	CYS331
A	GLY326
A	GLY328
A	CYS331

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
C	SER122
D	SER122
E	SER122
F	SER122
G	SER122
H	SER122
A	SER122
B	SER122

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
D	SER160
E	SER160
F	SER160
G	SER160
H	SER160
A	SER160
B	SER160
C	SER160

site_id	SWS_FT_FI8
Number of Residues	8
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:15592455

Chain	Residue	Details
B	TYR400
C	TYR400
D	TYR400
E	TYR400
F	TYR400
G	TYR400
H	TYR400
A	TYR400

site_id	SWS_FT_FI9
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:23186163, ECO:0007744\|PubMed:24275569

Chain	Residue	Details
B	SER416
C	SER416
D	SER416
E	SER416
F	SER416
G	SER416
H	SER416
A	SER416

site_id	SWS_FT_FI10
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
B	LYS511
D	LYS511
E	LYS511
F	LYS511
G	LYS511
H	LYS511
A	LYS511
C	LYS511

site_id	SWS_FT_FI11
Number of Residues	32
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
C	LYS195
C	LYS208
C	LYS438
D	LYS195
D	LYS208
D	LYS438
E	LYS195
E	LYS208
E	LYS438
F	LYS195
F	LYS208
F	LYS438
G	LYS195
G	LYS208
G	LYS438
H	LYS195
H	LYS208
H	LYS438
A	LYS195
A	LYS208
A	LYS438
B	LYS195
B	LYS208
B	LYS438

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)