6SYF

Human Ubc9 with covalent isopeptide ligand

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000166	molecular_function	nucleotide binding
A	0000795	cellular_component	synaptonemal complex
A	0001221	molecular_function	transcription coregulator binding
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005635	cellular_component	nuclear envelope
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0007059	biological_process	chromosome segregation
A	0007084	biological_process	mitotic nuclear membrane reassembly
A	0008134	molecular_function	transcription factor binding
A	0016604	cellular_component	nuclear body
A	0016605	cellular_component	PML body
A	0016740	molecular_function	transferase activity
A	0016925	biological_process	protein sumoylation
A	0019789	molecular_function	SUMO transferase activity
A	0019899	molecular_function	enzyme binding
A	0030335	biological_process	positive regulation of cell migration
A	0036211	biological_process	protein modification process
A	0043398	molecular_function	HLH domain binding
A	0044388	molecular_function	small protein activating enzyme binding
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050804	biological_process	modulation of chemical synaptic transmission
A	0051168	biological_process	nuclear export
A	0051170	biological_process	import into nucleus
A	0051301	biological_process	cell division
A	0061656	molecular_function	SUMO conjugating enzyme activity
A	0071535	molecular_function	RING-like zinc finger domain binding
A	0098685	cellular_component	Schaffer collateral - CA1 synapse
A	0098978	cellular_component	glutamatergic synapse
A	0099523	cellular_component	presynaptic cytosol
A	0099524	cellular_component	postsynaptic cytosol
A	0106068	cellular_component	SUMO ligase complex
A	1990234	cellular_component	transferase complex
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000166	molecular_function	nucleotide binding
B	0000795	cellular_component	synaptonemal complex
B	0001221	molecular_function	transcription coregulator binding
B	0003723	molecular_function	RNA binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005635	cellular_component	nuclear envelope
B	0005654	cellular_component	nucleoplasm
B	0005730	cellular_component	nucleolus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0007059	biological_process	chromosome segregation
B	0007084	biological_process	mitotic nuclear membrane reassembly
B	0008134	molecular_function	transcription factor binding
B	0016604	cellular_component	nuclear body
B	0016605	cellular_component	PML body
B	0016740	molecular_function	transferase activity
B	0016925	biological_process	protein sumoylation
B	0019789	molecular_function	SUMO transferase activity
B	0019899	molecular_function	enzyme binding
B	0030335	biological_process	positive regulation of cell migration
B	0036211	biological_process	protein modification process
B	0043398	molecular_function	HLH domain binding
B	0044388	molecular_function	small protein activating enzyme binding
B	0045892	biological_process	negative regulation of DNA-templated transcription
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050804	biological_process	modulation of chemical synaptic transmission
B	0051168	biological_process	nuclear export
B	0051170	biological_process	import into nucleus
B	0051301	biological_process	cell division
B	0061656	molecular_function	SUMO conjugating enzyme activity
B	0071535	molecular_function	RING-like zinc finger domain binding
B	0098685	cellular_component	Schaffer collateral - CA1 synapse
B	0098978	cellular_component	glutamatergic synapse
B	0099523	cellular_component	presynaptic cytosol
B	0099524	cellular_component	postsynaptic cytosol
B	0106068	cellular_component	SUMO ligase complex
B	1990234	cellular_component	transferase complex
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000166	molecular_function	nucleotide binding
C	0000795	cellular_component	synaptonemal complex
C	0001221	molecular_function	transcription coregulator binding
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005635	cellular_component	nuclear envelope
C	0005654	cellular_component	nucleoplasm
C	0005730	cellular_component	nucleolus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0007059	biological_process	chromosome segregation
C	0007084	biological_process	mitotic nuclear membrane reassembly
C	0008134	molecular_function	transcription factor binding
C	0016604	cellular_component	nuclear body
C	0016605	cellular_component	PML body
C	0016740	molecular_function	transferase activity
C	0016925	biological_process	protein sumoylation
C	0019789	molecular_function	SUMO transferase activity
C	0019899	molecular_function	enzyme binding
C	0030335	biological_process	positive regulation of cell migration
C	0036211	biological_process	protein modification process
C	0043398	molecular_function	HLH domain binding
C	0044388	molecular_function	small protein activating enzyme binding
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0050804	biological_process	modulation of chemical synaptic transmission
C	0051168	biological_process	nuclear export
C	0051170	biological_process	import into nucleus
C	0051301	biological_process	cell division
C	0061656	molecular_function	SUMO conjugating enzyme activity
C	0071535	molecular_function	RING-like zinc finger domain binding
C	0098685	cellular_component	Schaffer collateral - CA1 synapse
C	0098978	cellular_component	glutamatergic synapse
C	0099523	cellular_component	presynaptic cytosol
C	0099524	cellular_component	postsynaptic cytosol
C	0106068	cellular_component	SUMO ligase complex
C	1990234	cellular_component	transferase complex
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0000166	molecular_function	nucleotide binding
D	0000795	cellular_component	synaptonemal complex
D	0001221	molecular_function	transcription coregulator binding
D	0003723	molecular_function	RNA binding
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005635	cellular_component	nuclear envelope
D	0005654	cellular_component	nucleoplasm
D	0005730	cellular_component	nucleolus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0007059	biological_process	chromosome segregation
D	0007084	biological_process	mitotic nuclear membrane reassembly
D	0008134	molecular_function	transcription factor binding
D	0016604	cellular_component	nuclear body
D	0016605	cellular_component	PML body
D	0016740	molecular_function	transferase activity
D	0016925	biological_process	protein sumoylation
D	0019789	molecular_function	SUMO transferase activity
D	0019899	molecular_function	enzyme binding
D	0030335	biological_process	positive regulation of cell migration
D	0036211	biological_process	protein modification process
D	0043398	molecular_function	HLH domain binding
D	0044388	molecular_function	small protein activating enzyme binding
D	0045892	biological_process	negative regulation of DNA-templated transcription
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0050804	biological_process	modulation of chemical synaptic transmission
D	0051168	biological_process	nuclear export
D	0051170	biological_process	import into nucleus
D	0051301	biological_process	cell division
D	0061656	molecular_function	SUMO conjugating enzyme activity
D	0071535	molecular_function	RING-like zinc finger domain binding
D	0098685	cellular_component	Schaffer collateral - CA1 synapse
D	0098978	cellular_component	glutamatergic synapse
D	0099523	cellular_component	presynaptic cytosol
D	0099524	cellular_component	postsynaptic cytosol
D	0106068	cellular_component	SUMO ligase complex
D	1990234	cellular_component	transferase complex

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. FHPNVyps.GtVCLsiL

Chain	Residue	Details
A	PHE82-LEU97

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	Region: {"description":"Interaction with SUMO1"}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Glycyl thioester intermediate"}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	10
Details	Site: {"description":"Interaction with RANBP2"}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	4
Details	Site: {"description":"Substrate binding"}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine; by CDK1","evidences":[{"source":"PubMed","id":"22509284","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25755297","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI10
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"PubMed","id":"25114211","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25218447","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25755297","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25772364","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	306
Details	Domain: {"description":"UBC core","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"N-acetylserine","evidences":[{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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