6SMR
A. thaliana serine hydroxymethyltransferase isoform 4 (AtSHMT4) in complex with methotrexate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004372 | molecular_function | glycine hydroxymethyltransferase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0007623 | biological_process | circadian rhythm |
| A | 0009506 | cellular_component | plasmodesma |
| A | 0016740 | molecular_function | transferase activity |
| A | 0019264 | biological_process | glycine biosynthetic process from L-serine |
| A | 0030170 | molecular_function | pyridoxal phosphate binding |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 1901149 | molecular_function | salicylic acid binding |
| B | 0004372 | molecular_function | glycine hydroxymethyltransferase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0007623 | biological_process | circadian rhythm |
| B | 0009506 | cellular_component | plasmodesma |
| B | 0016740 | molecular_function | transferase activity |
| B | 0019264 | biological_process | glycine biosynthetic process from L-serine |
| B | 0030170 | molecular_function | pyridoxal phosphate binding |
| B | 0035999 | biological_process | tetrahydrofolate interconversion |
| B | 0046653 | biological_process | tetrahydrofolate metabolic process |
| B | 1901149 | molecular_function | salicylic acid binding |
| C | 0004372 | molecular_function | glycine hydroxymethyltransferase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006730 | biological_process | one-carbon metabolic process |
| C | 0007623 | biological_process | circadian rhythm |
| C | 0009506 | cellular_component | plasmodesma |
| C | 0016740 | molecular_function | transferase activity |
| C | 0019264 | biological_process | glycine biosynthetic process from L-serine |
| C | 0030170 | molecular_function | pyridoxal phosphate binding |
| C | 0035999 | biological_process | tetrahydrofolate interconversion |
| C | 0046653 | biological_process | tetrahydrofolate metabolic process |
| C | 1901149 | molecular_function | salicylic acid binding |
| D | 0004372 | molecular_function | glycine hydroxymethyltransferase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0006730 | biological_process | one-carbon metabolic process |
| D | 0007623 | biological_process | circadian rhythm |
| D | 0009506 | cellular_component | plasmodesma |
| D | 0016740 | molecular_function | transferase activity |
| D | 0019264 | biological_process | glycine biosynthetic process from L-serine |
| D | 0030170 | molecular_function | pyridoxal phosphate binding |
| D | 0035999 | biological_process | tetrahydrofolate interconversion |
| D | 0046653 | biological_process | tetrahydrofolate metabolic process |
| D | 1901149 | molecular_function | salicylic acid binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 18 |
| Details | binding site for residue PLS A 501 |
| Chain | Residue |
| A | SER39 |
| A | THR241 |
| A | HIS243 |
| A | LYS244 |
| A | ARG389 |
| A | HOH654 |
| B | TYR59 |
| B | GLU61 |
| B | GLY289 |
| B | GLY290 |
| A | SER105 |
| A | GLY106 |
| A | SER107 |
| A | HIS134 |
| A | GLY189 |
| A | SER190 |
| A | ASP215 |
| A | HIS218 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 502 |
| Chain | Residue |
| A | PHE180 |
| A | ARG181 |
| A | LYS183 |
| D | GLY144 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue MTX A 503 |
| Chain | Residue |
| A | TYR68 |
| B | TYR139 |
| B | ILE147 |
| B | LYS373 |
| B | SER381 |
| B | LEU383 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 504 |
| Chain | Residue |
| A | ALA286 |
| A | LEU287 |
| A | HOH704 |
| B | SER107 |
| B | ASN110 |
| B | PHE111 |
| B | THR136 |
| B | THR150 |
| site_id | AC5 |
| Number of Residues | 11 |
| Details | binding site for residue MTX B 501 |
| Chain | Residue |
| A | TYR139 |
| A | THR141 |
| A | SER142 |
| A | GLY143 |
| A | ILE147 |
| A | LYS373 |
| A | ALA382 |
| A | LEU383 |
| B | ARG67 |
| B | TYR68 |
| B | PRO285 |
| site_id | AC6 |
| Number of Residues | 19 |
| Details | binding site for residue PLS B 502 |
| Chain | Residue |
| A | TYR59 |
| A | GLU61 |
| A | TYR69 |
| A | GLY289 |
| A | GLY290 |
| B | SER39 |
| B | SER105 |
| B | GLY106 |
| B | SER107 |
| B | HIS134 |
| B | GLY189 |
| B | SER190 |
| B | ASP215 |
| B | HIS218 |
| B | THR241 |
| B | HIS243 |
| B | LYS244 |
| B | ARG389 |
| B | HOH742 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 503 |
| Chain | Residue |
| A | ILE48 |
| A | GLY52 |
| B | GLY52 |
| B | HOH625 |
| B | HOH695 |
| B | HOH741 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 504 |
| Chain | Residue |
| B | LYS176 |
| B | ASP179 |
| C | LEU157 |
| C | ASP179 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 505 |
| Chain | Residue |
| A | SER53 |
| A | HOH679 |
| B | ILE23 |
| B | GLU24 |
| B | HOH675 |
| B | HOH828 |
| site_id | AD1 |
| Number of Residues | 18 |
| Details | binding site for residue PLS C 501 |
| Chain | Residue |
| D | GLY290 |
| C | SER39 |
| C | SER105 |
| C | GLY106 |
| C | SER107 |
| C | HIS134 |
| C | SER190 |
| C | ASP215 |
| C | ALA217 |
| C | HIS218 |
| C | THR241 |
| C | HIS243 |
| C | LYS244 |
| C | ARG389 |
| D | TYR59 |
| D | GLU61 |
| D | TYR69 |
| D | GLY289 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 502 |
| Chain | Residue |
| C | HIS20 |
| C | ILE23 |
| C | GLU24 |
| C | HOH665 |
| C | HOH678 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue TRS C 503 |
| Chain | Residue |
| B | ASP449 |
| C | ARG194 |
| C | GLY340 |
| C | THR341 |
| C | HOH701 |
| C | HOH713 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue EDO C 504 |
| Chain | Residue |
| C | GLY52 |
| C | HOH623 |
| C | HOH668 |
| D | ILE48 |
| D | GLU49 |
| D | GLY52 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue EDO D 701 |
| Chain | Residue |
| A | LEU157 |
| D | LEU157 |
| D | LYS176 |
| D | ASP179 |
| site_id | AD6 |
| Number of Residues | 18 |
| Details | binding site for residue PLS D 702 |
| Chain | Residue |
| C | TYR59 |
| C | GLU61 |
| C | GLY289 |
| C | GLY290 |
| D | SER39 |
| D | SER105 |
| D | GLY106 |
| D | SER107 |
| D | HIS134 |
| D | GLY189 |
| D | SER190 |
| D | ASP215 |
| D | ALA217 |
| D | HIS218 |
| D | THR241 |
| D | HIS243 |
| D | LYS244 |
| D | ARG389 |
| site_id | AD7 |
| Number of Residues | 12 |
| Details | binding site for residue MTX D 703 |
| Chain | Residue |
| C | ARG67 |
| C | TYR68 |
| D | THR141 |
| D | SER142 |
| D | GLY143 |
| D | ILE147 |
| D | LYS373 |
| D | SER380 |
| D | SER381 |
| D | ALA382 |
| D | LEU383 |
| D | HOH831 |
| site_id | AD8 |
| Number of Residues | 7 |
| Details | binding site for residue EDO D 704 |
| Chain | Residue |
| D | GLY189 |
| D | SER190 |
| D | ALA191 |
| D | TYR192 |
| D | TRP196 |
| D | HOH811 |
| D | HOH817 |
Functional Information from PROSITE/UniProt
| site_id | PS00096 |
| Number of Residues | 17 |
| Details | SHMT Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. DVvTTTTHKSLrGPRAG |
| Chain | Residue | Details |
| A | ASP236-GLY252 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 44 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"31873125","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6SMR","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"Q94C74","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"22223895","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
