6RZB

Cryo-EM structure of mouse cytoplasmic dynein-1 microtubule binding domain bound to microtubules

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0003924	molecular_function	GTPase activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0007017	biological_process	microtubule-based process
A	0015630	cellular_component	microtubule cytoskeleton
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0003924	molecular_function	GTPase activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0007017	biological_process	microtubule-based process
B	0046872	molecular_function	metal ion binding
C	0007018	biological_process	microtubule-based movement
C	0030286	cellular_component	dynein complex
C	0045505	molecular_function	dynein intermediate chain binding
C	0051959	molecular_function	dynein light intermediate chain binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	binding site for residue GTP A 501

Chain	Residue
A	GLY10
A	ASN101
A	SER140
A	GLY143
A	GLY144
A	THR145
A	GLY146
A	ILE171
A	THR179
A	ASN206
A	TYR224
A	GLN11
A	LEU227
A	ASN228
A	MG502
B	LYS252
A	ALA12
A	GLN15
A	ILE16
A	GLU71
A	ASP98
A	ALA99
A	ALA100

---

site_id	AC2
Number of Residues	3
Details	binding site for residue MG A 502

Chain	Residue
A	GLN11
A	GLU71
A	GTP501

---

site_id	AC3
Number of Residues	13
Details	binding site for residue GDP B 501

Chain	Residue
B	GLY10
B	GLN11
B	CYS12
B	GLN15
B	ASN99
B	GLY141
B	GLY142
B	THR143
B	ASP177
B	GLU181
B	ASN204
B	TYR222
B	ASN226

---

site_id	AC4
Number of Residues	14
Details	binding site for residue TA1 B 502

Chain	Residue
B	GLU22
B	VAL23
B	ASP26
B	ASP224
B	HIS227
B	LEU228
B	ALA231
B	PRO272
B	THR274
B	ARG276
B	ARG318
B	PRO358
B	ARG359
B	LEU361

---

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
A	GLY142-GLY148
B	GLY140-GLY146

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
B	MET1-ILE4

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	7
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q13509

Chain	Residue	Details
B	GLN11
B	SER138
B	GLY142
B	THR143
B	GLY144
B	ASN204
B	ASN226

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
B	GLU69
A	GLU71
A	SER140
A	GLY144
A	THR145
A	THR179
A	ASN206
A	ASN228

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
B	SER40

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
B	LYS58
A	SER232

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine; by CDK1 => ECO:0000250|UniProtKB:Q13885

Chain	Residue	Details
B	SER172

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	THR285
B	THR290

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Omega-N-methylarginine => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	ARG318
A	LYS370

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	LYS58

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	LYS324

---