6RJB
Human transketolase variant T382E
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004802 | molecular_function | transketolase activity |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005777 | cellular_component | peroxisome |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0006098 | biological_process | pentose-phosphate shunt |
| A | 0009052 | biological_process | pentose-phosphate shunt, non-oxidative branch |
| A | 0016604 | cellular_component | nuclear body |
| A | 0016740 | molecular_function | transferase activity |
| A | 0030976 | molecular_function | thiamine pyrophosphate binding |
| A | 0031982 | cellular_component | vesicle |
| A | 0040008 | biological_process | regulation of growth |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 1901159 | biological_process | xylulose 5-phosphate biosynthetic process |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004802 | molecular_function | transketolase activity |
| B | 0005509 | molecular_function | calcium ion binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005777 | cellular_component | peroxisome |
| B | 0005789 | cellular_component | endoplasmic reticulum membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0006098 | biological_process | pentose-phosphate shunt |
| B | 0009052 | biological_process | pentose-phosphate shunt, non-oxidative branch |
| B | 0016604 | cellular_component | nuclear body |
| B | 0016740 | molecular_function | transferase activity |
| B | 0030976 | molecular_function | thiamine pyrophosphate binding |
| B | 0031982 | cellular_component | vesicle |
| B | 0040008 | biological_process | regulation of growth |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 1901159 | biological_process | xylulose 5-phosphate biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 27 |
| Details | binding site for residue TPP A 701 |
| Chain | Residue |
| A | SER40 |
| A | GLU157 |
| A | GLU160 |
| A | ASN185 |
| A | LEU187 |
| A | GLY188 |
| A | GLN189 |
| A | LYS244 |
| A | HIS258 |
| A | MG702 |
| A | CA703 |
| A | LYS75 |
| A | HOH964 |
| A | HOH1288 |
| B | ASP341 |
| B | ILE364 |
| B | GLU366 |
| B | PHE392 |
| B | ARG395 |
| B | GLN428 |
| A | HIS77 |
| A | GLY123 |
| A | SER124 |
| A | LEU125 |
| A | GLY154 |
| A | ASP155 |
| A | GLY156 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 702 |
| Chain | Residue |
| A | ASP155 |
| A | ASN185 |
| A | LEU187 |
| A | TPP701 |
| A | HOH964 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue CA A 703 |
| Chain | Residue |
| A | ASP155 |
| A | ASN185 |
| A | LEU187 |
| A | TPP701 |
| A | HOH964 |
| site_id | AC4 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 704 |
| Chain | Residue |
| A | PRO475 |
| A | ASN477 |
| A | VAL510 |
| A | PRO598 |
| A | LEU602 |
| A | HOH960 |
| A | HOH1125 |
| A | HOH1188 |
| A | HOH1295 |
| A | HOH1382 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 705 |
| Chain | Residue |
| A | PRO63 |
| A | ASN68 |
| A | ASP69 |
| A | ARG70 |
| A | PHE71 |
| A | ARG379 |
| A | HOH855 |
| site_id | AC6 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 706 |
| Chain | Residue |
| A | PHE71 |
| A | VAL72 |
| A | LEU73 |
| A | LEU82 |
| A | PHE117 |
| A | HOH857 |
| A | HOH1040 |
| A | HOH1171 |
| site_id | AC7 |
| Number of Residues | 11 |
| Details | binding site for residue EDO A 707 |
| Chain | Residue |
| A | LEU158 |
| A | SER159 |
| A | TRP164 |
| A | LEU195 |
| A | ARG205 |
| A | EDO711 |
| A | HOH977 |
| A | HOH985 |
| B | PHE209 |
| B | PEG725 |
| B | HOH920 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 708 |
| Chain | Residue |
| A | TYR564 |
| A | ALA588 |
| A | ASN590 |
| A | HOH867 |
| A | HOH1013 |
| A | HOH1128 |
| A | HOH1221 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 709 |
| Chain | Residue |
| A | GLN189 |
| A | SER256 |
| A | TRP257 |
| A | HIS258 |
| A | HOH1065 |
| A | HOH1166 |
| B | ASN344 |
| site_id | AD1 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 710 |
| Chain | Residue |
| A | TYR563 |
| A | TYR564 |
| A | GLU565 |
| A | VAL589 |
| A | ASN590 |
| A | HOH1206 |
| B | PRO441 |
| B | LYS538 |
| site_id | AD2 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 711 |
| Chain | Residue |
| B | TRP164 |
| B | GLU165 |
| B | PHE209 |
| B | PEG725 |
| A | LEU158 |
| A | SER159 |
| A | TRP164 |
| A | EDO707 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 712 |
| Chain | Residue |
| A | ARG21 |
| A | ALA84 |
| A | GLU88 |
| A | GLU94 |
| A | LEU97 |
| A | HOH1256 |
| site_id | AD4 |
| Number of Residues | 9 |
| Details | binding site for residue EDO A 713 |
| Chain | Residue |
| A | VAL120 |
| A | GLN127 |
| A | ALA131 |
| A | SER371 |
| A | VAL374 |
| A | GLN399 |
| A | MET402 |
| A | ALA403 |
| A | HOH823 |
| site_id | AD5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 714 |
| Chain | Residue |
| A | GLU423 |
| A | LYS597 |
| A | HOH840 |
| A | HOH884 |
| A | HOH1234 |
| B | ALA33 |
| B | ARG101 |
| site_id | AD6 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 715 |
| Chain | Residue |
| A | LEU92 |
| A | ASP106 |
| A | LEU107 |
| A | GLN115 |
| A | EDO719 |
| A | HOH875 |
| A | HOH890 |
| A | HOH899 |
| site_id | AD7 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 716 |
| Chain | Residue |
| A | TYR137 |
| A | TYR173 |
| A | HOH929 |
| A | HOH1088 |
| A | HOH1091 |
| A | HOH1212 |
| site_id | AD8 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 717 |
| Chain | Residue |
| A | ALA326 |
| A | LYS327 |
| A | HIS330 |
| A | GLU354 |
| A | HOH917 |
| site_id | AD9 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 718 |
| Chain | Residue |
| A | PRO307 |
| A | SER308 |
| A | TYR309 |
| A | LYS327 |
| A | GLU455 |
| A | HOH828 |
| A | HOH833 |
| site_id | AE1 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 719 |
| Chain | Residue |
| A | PHE91 |
| A | LEU92 |
| A | EDO715 |
| A | HOH1012 |
| A | HOH1059 |
| site_id | AE2 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 720 |
| Chain | Residue |
| A | ASP217 |
| A | HOH856 |
| A | HOH1034 |
| site_id | AE3 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 721 |
| Chain | Residue |
| A | ASP217 |
| A | HIS219 |
| A | LYS241 |
| site_id | AE4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 722 |
| Chain | Residue |
| A | ARG246 |
| A | GLY247 |
| A | GLU252 |
| A | HOH845 |
| A | HOH967 |
| site_id | AE5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 723 |
| Chain | Residue |
| A | ALA462 |
| A | ASN463 |
| A | THR464 |
| A | LYS465 |
| A | HOH939 |
| site_id | AE6 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 724 |
| Chain | Residue |
| A | ARG318 |
| A | SER345 |
| A | HIS416 |
| A | ARG474 |
| A | HOH1047 |
| site_id | AE7 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 725 |
| Chain | Residue |
| A | ASN411 |
| A | ALA461 |
| A | THR464 |
| A | CYS468 |
| A | HOH844 |
| A | HOH1143 |
| site_id | AE8 |
| Number of Residues | 8 |
| Details | binding site for residue NA A 726 |
| Chain | Residue |
| A | SER74 |
| A | THR122 |
| A | SER124 |
| A | LEU125 |
| A | GLY126 |
| A | GLN127 |
| A | GLY128 |
| A | GLU157 |
| site_id | AE9 |
| Number of Residues | 10 |
| Details | binding site for residue PEG A 727 |
| Chain | Residue |
| A | GLU165 |
| A | ALA168 |
| A | PHE169 |
| A | ILE172 |
| A | CYS362 |
| A | ASN368 |
| B | SER159 |
| B | EDO705 |
| B | EDO708 |
| B | HOH1054 |
| site_id | AF1 |
| Number of Residues | 27 |
| Details | binding site for residue TPP B 701 |
| Chain | Residue |
| A | ASP341 |
| A | ILE364 |
| A | GLU366 |
| A | PHE392 |
| A | ARG395 |
| A | GLN428 |
| A | HOH803 |
| B | SER40 |
| B | LYS75 |
| B | HIS77 |
| B | GLY123 |
| B | SER124 |
| B | LEU125 |
| B | GLY154 |
| B | ASP155 |
| B | GLY156 |
| B | GLU157 |
| B | GLU160 |
| B | ASN185 |
| B | LEU187 |
| B | GLY188 |
| B | GLN189 |
| B | LYS244 |
| B | HIS258 |
| B | MG702 |
| B | CA703 |
| B | HOH948 |
| site_id | AF2 |
| Number of Residues | 5 |
| Details | binding site for residue MG B 702 |
| Chain | Residue |
| B | ASP155 |
| B | ASN185 |
| B | LEU187 |
| B | TPP701 |
| B | HOH948 |
| site_id | AF3 |
| Number of Residues | 5 |
| Details | binding site for residue CA B 703 |
| Chain | Residue |
| B | ASP155 |
| B | ASN185 |
| B | LEU187 |
| B | TPP701 |
| B | HOH948 |
| site_id | AF4 |
| Number of Residues | 7 |
| Details | binding site for residue GOL B 704 |
| Chain | Residue |
| B | ARG318 |
| B | ASN344 |
| B | SER345 |
| B | HIS416 |
| B | ARG474 |
| B | HOH1019 |
| B | HOH1043 |
| site_id | AF5 |
| Number of Residues | 12 |
| Details | binding site for residue EDO B 705 |
| Chain | Residue |
| A | PHE209 |
| A | PEG727 |
| B | LEU158 |
| B | SER159 |
| B | TRP164 |
| B | LEU195 |
| B | TYR202 |
| B | ARG205 |
| B | EDO708 |
| B | HOH975 |
| B | HOH996 |
| B | HOH1054 |
| site_id | AF6 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 706 |
| Chain | Residue |
| B | PHE71 |
| B | VAL72 |
| B | LEU73 |
| B | LEU82 |
| B | PHE117 |
| B | HOH818 |
| B | HOH1037 |
| B | HOH1135 |
| site_id | AF7 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 707 |
| Chain | Residue |
| A | PRO441 |
| A | LYS538 |
| B | TYR563 |
| B | TYR564 |
| B | GLU565 |
| B | VAL589 |
| B | ASN590 |
| B | HOH1126 |
| site_id | AF8 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 708 |
| Chain | Residue |
| A | GLU165 |
| A | PHE209 |
| A | PEG727 |
| B | LEU158 |
| B | SER159 |
| B | TRP164 |
| B | EDO705 |
| site_id | AF9 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 709 |
| Chain | Residue |
| A | ASN344 |
| B | GLN189 |
| B | SER256 |
| B | TRP257 |
| B | HIS258 |
| B | HOH1115 |
| B | HOH1145 |
| site_id | AG1 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 710 |
| Chain | Residue |
| A | ALA33 |
| A | ARG101 |
| A | LYS102 |
| B | GLU423 |
| B | LYS597 |
| B | HOH860 |
| B | HOH998 |
| site_id | AG2 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 711 |
| Chain | Residue |
| B | PRO63 |
| B | ASN68 |
| B | ASP69 |
| B | ARG70 |
| B | PHE71 |
| B | ARG379 |
| B | HOH872 |
| site_id | AG3 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 712 |
| Chain | Residue |
| B | TYR564 |
| B | ALA588 |
| B | ASN590 |
| B | HOH856 |
| B | HOH935 |
| B | HOH1036 |
| B | HOH1156 |
| site_id | AG4 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 713 |
| Chain | Residue |
| B | LEU92 |
| B | LEU107 |
| B | GLN115 |
| B | HOH857 |
| B | HOH914 |
| B | HOH924 |
| site_id | AG5 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 714 |
| Chain | Residue |
| B | PRO475 |
| B | ASN477 |
| B | VAL510 |
| B | PRO598 |
| B | HOH807 |
| B | HOH870 |
| B | HOH995 |
| B | HOH1171 |
| site_id | AG6 |
| Number of Residues | 3 |
| Details | binding site for residue EDO B 715 |
| Chain | Residue |
| B | ASN590 |
| B | ARG591 |
| B | HOH838 |
| site_id | AG7 |
| Number of Residues | 9 |
| Details | binding site for residue EDO B 716 |
| Chain | Residue |
| B | ARG21 |
| B | SER25 |
| B | ALA84 |
| B | ALA87 |
| B | GLU88 |
| B | GLU94 |
| B | LEU97 |
| B | LYS283 |
| B | HOH843 |
| site_id | AG8 |
| Number of Residues | 1 |
| Details | binding site for residue EDO B 717 |
| Chain | Residue |
| B | GLN10 |
| site_id | AG9 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 718 |
| Chain | Residue |
| B | MET199 |
| B | ASP217 |
| B | HOH811 |
| B | HOH1020 |
| site_id | AH1 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 719 |
| Chain | Residue |
| B | ALA316 |
| B | THR317 |
| B | SER449 |
| B | SER473 |
| B | PRO475 |
| B | ASN477 |
| B | GLY509 |
| B | VAL510 |
| site_id | AH2 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 720 |
| Chain | Residue |
| B | ALA267 |
| B | GLU268 |
| B | HOH951 |
| B | HOH1078 |
| site_id | AH3 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 721 |
| Chain | Residue |
| A | ARG205 |
| A | HOH1332 |
| B | ALA168 |
| B | SER171 |
| B | ILE172 |
| B | HOH920 |
| B | HOH1292 |
| site_id | AH4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 722 |
| Chain | Residue |
| B | ASP217 |
| B | HIS219 |
| B | LYS241 |
| B | HOH811 |
| B | HOH909 |
| site_id | AH5 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 723 |
| Chain | Residue |
| B | ASN300 |
| B | ILE301 |
| B | ARG302 |
| B | LEU460 |
| B | ASN463 |
| B | HOH825 |
| site_id | AH6 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 724 |
| Chain | Residue |
| B | VAL120 |
| B | GLN127 |
| B | SER371 |
| B | VAL374 |
| B | GLN399 |
| B | MET402 |
| B | ALA403 |
| B | HOH839 |
| site_id | AH7 |
| Number of Residues | 11 |
| Details | binding site for residue PEG B 725 |
| Chain | Residue |
| A | SER159 |
| A | EDO707 |
| A | EDO711 |
| B | GLU165 |
| B | ALA168 |
| B | PHE169 |
| B | ILE172 |
| B | CYS362 |
| B | ALA365 |
| B | ASN368 |
| B | HOH920 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000250 |
| Chain | Residue | Details |
| A | GLU366 | |
| B | GLU366 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | HIS37 | |
| B | HIS416 | |
| B | ASP424 | |
| B | ARG474 | |
| A | ARG318 | |
| A | SER345 | |
| A | HIS416 | |
| A | ASP424 | |
| A | ARG474 | |
| B | HIS37 | |
| B | ARG318 | |
| B | SER345 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 22 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | SER40 | |
| A | PHE392 | |
| A | GLN428 | |
| B | SER40 | |
| B | HIS77 | |
| B | GLY123 | |
| B | ASP155 | |
| B | GLY156 | |
| B | ASN185 | |
| B | LEU187 | |
| B | LYS244 | |
| A | HIS77 | |
| B | HIS258 | |
| B | PHE392 | |
| B | GLN428 | |
| A | GLY123 | |
| A | ASP155 | |
| A | GLY156 | |
| A | ASN185 | |
| A | LEU187 | |
| A | LYS244 | |
| A | HIS258 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | SITE: Important for catalytic activity => ECO:0000250 |
| Chain | Residue | Details |
| A | HIS37 | |
| A | HIS258 | |
| B | HIS37 | |
| B | HIS258 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| A | MET1 | |
| B | MET1 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER3 | |
| A | SER104 | |
| B | SER3 | |
| B | SER104 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 14 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS6 | |
| B | LYS144 | |
| B | LYS204 | |
| B | LYS241 | |
| B | LYS260 | |
| B | LYS603 | |
| A | LYS11 | |
| A | LYS144 | |
| A | LYS204 | |
| A | LYS241 | |
| A | LYS260 | |
| A | LYS603 | |
| B | LYS6 | |
| B | LYS11 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P40142 |
| Chain | Residue | Details |
| A | LYS232 | |
| A | LYS538 | |
| B | LYS232 | |
| B | LYS538 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231 |
| Chain | Residue | Details |
| A | TYR275 | |
| B | TYR275 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | THR287 | |
| B | THR287 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER295 | |
| B | SER295 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P50137 |
| Chain | Residue | Details |
| A | SER345 | |
| B | SER345 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| A | LYS352 | |
| B | LYS352 |
