6R51

Crystal structure of apo PPEP-1(E143A/Y178F) in complex with fibrinogen-derived substrate peptide Ac-SLRPAPP-CONH2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0006508	biological_process	proteolysis
A	0008237	molecular_function	metallopeptidase activity
A	0046872	molecular_function	metal ion binding
B	0005576	cellular_component	extracellular region
B	0006508	biological_process	proteolysis
B	0008237	molecular_function	metallopeptidase activity
B	0046872	molecular_function	metal ion binding
C	0005576	cellular_component	extracellular region
C	0006508	biological_process	proteolysis
C	0008237	molecular_function	metallopeptidase activity
C	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue PO4 A 301

Chain	Residue
A	HIS25
A	TYR194
A	HOH402
A	HOH412
A	HOH425
A	HOH471

---

site_id	AC2
Number of Residues	8
Details	binding site for Di-peptide ACE D 31 and SER D 32

Chain	Residue
D	LEU33
D	ARG34
D	HOH101
F	ACE31
F	SER32
A	GLY117
A	GLY118
A	SER119

---

site_id	AC3
Number of Residues	10
Details	binding site for Di-peptide PRO D 37 and LPD D 38

Chain	Residue
A	GLY102
A	TRP103
A	HIS134
A	ASP135
A	ALA136
A	HIS142
A	ASN175
A	PHE178
A	HOH513
D	ALA36

---

site_id	AC4
Number of Residues	9
Details	binding site for Di-peptide ACE F 31 and SER F 32

Chain	Residue
C	GLY117
C	GLY118
C	SER119
C	HIS150
D	ACE31
D	SER32
F	LEU33
F	ARG34
F	HOH102

---

site_id	AC5
Number of Residues	8
Details	binding site for Di-peptide PRO F 37 and LPD F 38

Chain	Residue
C	GLY102
C	TRP103
C	HIS134
C	ASP135
C	ALA136
C	HIS142
C	PHE178
F	ALA36

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU01339, ECO:0000305|PubMed:24303041, ECO:0000305|PubMed:26211609

Chain	Residue	Details
B	ALA143
A	ALA143
C	ALA143

---

site_id	SWS_FT_FI2
Number of Residues	9
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01339, ECO:0000269|PubMed:26211609

Chain	Residue	Details
B	HIS142
B	HIS146
B	GLU185
A	HIS142
A	HIS146
A	GLU185
C	HIS142
C	HIS146
C	GLU185

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01339

Chain	Residue	Details
B	PHE178
A	PHE178
C	PHE178

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	SITE: Interacts with substrate peptide => ECO:0000269|PubMed:26211609

Chain	Residue	Details
B	ASP135
B	HIS142
A	ASP135
A	HIS142
C	ASP135
C	HIS142

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	SITE: Transition state stabilizer => ECO:0000305|PubMed:26211609

Chain	Residue	Details
B	PHE178
A	PHE178
C	PHE178

---