6R2E

Crystal structure of the human thymidylate synthase (hTS) interface variant Q62R

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0003729	molecular_function	mRNA binding
A	0004799	molecular_function	thymidylate synthase activity
A	0005542	molecular_function	folic acid binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005759	cellular_component	mitochondrial matrix
A	0005829	cellular_component	cytosol
A	0006206	biological_process	pyrimidine nucleobase metabolic process
A	0006231	biological_process	dTMP biosynthetic process
A	0006235	biological_process	dTTP biosynthetic process
A	0006417	biological_process	regulation of translation
A	0007623	biological_process	circadian rhythm
A	0008168	molecular_function	methyltransferase activity
A	0009165	biological_process	nucleotide biosynthetic process
A	0009410	biological_process	response to xenobiotic stimulus
A	0009636	biological_process	response to toxic substance
A	0014070	biological_process	response to organic cyclic compound
A	0016741	molecular_function	transferase activity, transferring one-carbon groups
A	0017148	biological_process	negative regulation of translation
A	0019860	biological_process	uracil metabolic process
A	0032259	biological_process	methylation
A	0032570	biological_process	response to progesterone
A	0033189	biological_process	response to vitamin A
A	0034097	biological_process	response to cytokine
A	0035999	biological_process	tetrahydrofolate interconversion
A	0042803	molecular_function	protein homodimerization activity
A	0045471	biological_process	response to ethanol
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0046683	biological_process	response to organophosphorus
A	0048589	biological_process	developmental growth
A	0051216	biological_process	cartilage development
A	0051384	biological_process	response to glucocorticoid
A	0051593	biological_process	response to folic acid
A	0060574	biological_process	intestinal epithelial cell maturation
A	0071897	biological_process	DNA biosynthetic process
A	0097421	biological_process	liver regeneration
A	1901363	molecular_function	heterocyclic compound binding
A	1990825	molecular_function	sequence-specific mRNA binding
B	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
B	0003729	molecular_function	mRNA binding
B	0004799	molecular_function	thymidylate synthase activity
B	0005542	molecular_function	folic acid binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005759	cellular_component	mitochondrial matrix
B	0005829	cellular_component	cytosol
B	0006206	biological_process	pyrimidine nucleobase metabolic process
B	0006231	biological_process	dTMP biosynthetic process
B	0006235	biological_process	dTTP biosynthetic process
B	0006417	biological_process	regulation of translation
B	0007623	biological_process	circadian rhythm
B	0008168	molecular_function	methyltransferase activity
B	0009165	biological_process	nucleotide biosynthetic process
B	0009410	biological_process	response to xenobiotic stimulus
B	0009636	biological_process	response to toxic substance
B	0014070	biological_process	response to organic cyclic compound
B	0016741	molecular_function	transferase activity, transferring one-carbon groups
B	0017148	biological_process	negative regulation of translation
B	0019860	biological_process	uracil metabolic process
B	0032259	biological_process	methylation
B	0032570	biological_process	response to progesterone
B	0033189	biological_process	response to vitamin A
B	0034097	biological_process	response to cytokine
B	0035999	biological_process	tetrahydrofolate interconversion
B	0042803	molecular_function	protein homodimerization activity
B	0045471	biological_process	response to ethanol
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0046683	biological_process	response to organophosphorus
B	0048589	biological_process	developmental growth
B	0051216	biological_process	cartilage development
B	0051384	biological_process	response to glucocorticoid
B	0051593	biological_process	response to folic acid
B	0060574	biological_process	intestinal epithelial cell maturation
B	0071897	biological_process	DNA biosynthetic process
B	0097421	biological_process	liver regeneration
B	1901363	molecular_function	heterocyclic compound binding
B	1990825	molecular_function	sequence-specific mRNA binding
C	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
C	0003729	molecular_function	mRNA binding
C	0004799	molecular_function	thymidylate synthase activity
C	0005542	molecular_function	folic acid binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0005759	cellular_component	mitochondrial matrix
C	0005829	cellular_component	cytosol
C	0006206	biological_process	pyrimidine nucleobase metabolic process
C	0006231	biological_process	dTMP biosynthetic process
C	0006235	biological_process	dTTP biosynthetic process
C	0006417	biological_process	regulation of translation
C	0007623	biological_process	circadian rhythm
C	0008168	molecular_function	methyltransferase activity
C	0009165	biological_process	nucleotide biosynthetic process
C	0009410	biological_process	response to xenobiotic stimulus
C	0009636	biological_process	response to toxic substance
C	0014070	biological_process	response to organic cyclic compound
C	0016741	molecular_function	transferase activity, transferring one-carbon groups
C	0017148	biological_process	negative regulation of translation
C	0019860	biological_process	uracil metabolic process
C	0032259	biological_process	methylation
C	0032570	biological_process	response to progesterone
C	0033189	biological_process	response to vitamin A
C	0034097	biological_process	response to cytokine
C	0035999	biological_process	tetrahydrofolate interconversion
C	0042803	molecular_function	protein homodimerization activity
C	0045471	biological_process	response to ethanol
C	0046653	biological_process	tetrahydrofolate metabolic process
C	0046683	biological_process	response to organophosphorus
C	0048589	biological_process	developmental growth
C	0051216	biological_process	cartilage development
C	0051384	biological_process	response to glucocorticoid
C	0051593	biological_process	response to folic acid
C	0060574	biological_process	intestinal epithelial cell maturation
C	0071897	biological_process	DNA biosynthetic process
C	0097421	biological_process	liver regeneration
C	1901363	molecular_function	heterocyclic compound binding
C	1990825	molecular_function	sequence-specific mRNA binding
D	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
D	0003729	molecular_function	mRNA binding
D	0004799	molecular_function	thymidylate synthase activity
D	0005542	molecular_function	folic acid binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0005759	cellular_component	mitochondrial matrix
D	0005829	cellular_component	cytosol
D	0006206	biological_process	pyrimidine nucleobase metabolic process
D	0006231	biological_process	dTMP biosynthetic process
D	0006235	biological_process	dTTP biosynthetic process
D	0006417	biological_process	regulation of translation
D	0007623	biological_process	circadian rhythm
D	0008168	molecular_function	methyltransferase activity
D	0009165	biological_process	nucleotide biosynthetic process
D	0009410	biological_process	response to xenobiotic stimulus
D	0009636	biological_process	response to toxic substance
D	0014070	biological_process	response to organic cyclic compound
D	0016741	molecular_function	transferase activity, transferring one-carbon groups
D	0017148	biological_process	negative regulation of translation
D	0019860	biological_process	uracil metabolic process
D	0032259	biological_process	methylation
D	0032570	biological_process	response to progesterone
D	0033189	biological_process	response to vitamin A
D	0034097	biological_process	response to cytokine
D	0035999	biological_process	tetrahydrofolate interconversion
D	0042803	molecular_function	protein homodimerization activity
D	0045471	biological_process	response to ethanol
D	0046653	biological_process	tetrahydrofolate metabolic process
D	0046683	biological_process	response to organophosphorus
D	0048589	biological_process	developmental growth
D	0051216	biological_process	cartilage development
D	0051384	biological_process	response to glucocorticoid
D	0051593	biological_process	response to folic acid
D	0060574	biological_process	intestinal epithelial cell maturation
D	0071897	biological_process	DNA biosynthetic process
D	0097421	biological_process	liver regeneration
D	1901363	molecular_function	heterocyclic compound binding
D	1990825	molecular_function	sequence-specific mRNA binding
E	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
E	0003729	molecular_function	mRNA binding
E	0004799	molecular_function	thymidylate synthase activity
E	0005542	molecular_function	folic acid binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0005759	cellular_component	mitochondrial matrix
E	0005829	cellular_component	cytosol
E	0006206	biological_process	pyrimidine nucleobase metabolic process
E	0006231	biological_process	dTMP biosynthetic process
E	0006235	biological_process	dTTP biosynthetic process
E	0006417	biological_process	regulation of translation
E	0007623	biological_process	circadian rhythm
E	0008168	molecular_function	methyltransferase activity
E	0009165	biological_process	nucleotide biosynthetic process
E	0009410	biological_process	response to xenobiotic stimulus
E	0009636	biological_process	response to toxic substance
E	0014070	biological_process	response to organic cyclic compound
E	0016741	molecular_function	transferase activity, transferring one-carbon groups
E	0017148	biological_process	negative regulation of translation
E	0019860	biological_process	uracil metabolic process
E	0032259	biological_process	methylation
E	0032570	biological_process	response to progesterone
E	0033189	biological_process	response to vitamin A
E	0034097	biological_process	response to cytokine
E	0035999	biological_process	tetrahydrofolate interconversion
E	0042803	molecular_function	protein homodimerization activity
E	0045471	biological_process	response to ethanol
E	0046653	biological_process	tetrahydrofolate metabolic process
E	0046683	biological_process	response to organophosphorus
E	0048589	biological_process	developmental growth
E	0051216	biological_process	cartilage development
E	0051384	biological_process	response to glucocorticoid
E	0051593	biological_process	response to folic acid
E	0060574	biological_process	intestinal epithelial cell maturation
E	0071897	biological_process	DNA biosynthetic process
E	0097421	biological_process	liver regeneration
E	1901363	molecular_function	heterocyclic compound binding
E	1990825	molecular_function	sequence-specific mRNA binding
F	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
F	0003729	molecular_function	mRNA binding
F	0004799	molecular_function	thymidylate synthase activity
F	0005542	molecular_function	folic acid binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0005759	cellular_component	mitochondrial matrix
F	0005829	cellular_component	cytosol
F	0006206	biological_process	pyrimidine nucleobase metabolic process
F	0006231	biological_process	dTMP biosynthetic process
F	0006235	biological_process	dTTP biosynthetic process
F	0006417	biological_process	regulation of translation
F	0007623	biological_process	circadian rhythm
F	0008168	molecular_function	methyltransferase activity
F	0009165	biological_process	nucleotide biosynthetic process
F	0009410	biological_process	response to xenobiotic stimulus
F	0009636	biological_process	response to toxic substance
F	0014070	biological_process	response to organic cyclic compound
F	0016741	molecular_function	transferase activity, transferring one-carbon groups
F	0017148	biological_process	negative regulation of translation
F	0019860	biological_process	uracil metabolic process
F	0032259	biological_process	methylation
F	0032570	biological_process	response to progesterone
F	0033189	biological_process	response to vitamin A
F	0034097	biological_process	response to cytokine
F	0035999	biological_process	tetrahydrofolate interconversion
F	0042803	molecular_function	protein homodimerization activity
F	0045471	biological_process	response to ethanol
F	0046653	biological_process	tetrahydrofolate metabolic process
F	0046683	biological_process	response to organophosphorus
F	0048589	biological_process	developmental growth
F	0051216	biological_process	cartilage development
F	0051384	biological_process	response to glucocorticoid
F	0051593	biological_process	response to folic acid
F	0060574	biological_process	intestinal epithelial cell maturation
F	0071897	biological_process	DNA biosynthetic process
F	0097421	biological_process	liver regeneration
F	1901363	molecular_function	heterocyclic compound binding
F	1990825	molecular_function	sequence-specific mRNA binding
G	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
G	0003729	molecular_function	mRNA binding
G	0004799	molecular_function	thymidylate synthase activity
G	0005542	molecular_function	folic acid binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005743	cellular_component	mitochondrial inner membrane
G	0005759	cellular_component	mitochondrial matrix
G	0005829	cellular_component	cytosol
G	0006206	biological_process	pyrimidine nucleobase metabolic process
G	0006231	biological_process	dTMP biosynthetic process
G	0006235	biological_process	dTTP biosynthetic process
G	0006417	biological_process	regulation of translation
G	0007623	biological_process	circadian rhythm
G	0008168	molecular_function	methyltransferase activity
G	0009165	biological_process	nucleotide biosynthetic process
G	0009410	biological_process	response to xenobiotic stimulus
G	0009636	biological_process	response to toxic substance
G	0014070	biological_process	response to organic cyclic compound
G	0016741	molecular_function	transferase activity, transferring one-carbon groups
G	0017148	biological_process	negative regulation of translation
G	0019860	biological_process	uracil metabolic process
G	0032259	biological_process	methylation
G	0032570	biological_process	response to progesterone
G	0033189	biological_process	response to vitamin A
G	0034097	biological_process	response to cytokine
G	0035999	biological_process	tetrahydrofolate interconversion
G	0042803	molecular_function	protein homodimerization activity
G	0045471	biological_process	response to ethanol
G	0046653	biological_process	tetrahydrofolate metabolic process
G	0046683	biological_process	response to organophosphorus
G	0048589	biological_process	developmental growth
G	0051216	biological_process	cartilage development
G	0051384	biological_process	response to glucocorticoid
G	0051593	biological_process	response to folic acid
G	0060574	biological_process	intestinal epithelial cell maturation
G	0071897	biological_process	DNA biosynthetic process
G	0097421	biological_process	liver regeneration
G	1901363	molecular_function	heterocyclic compound binding
G	1990825	molecular_function	sequence-specific mRNA binding
H	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
H	0003729	molecular_function	mRNA binding
H	0004799	molecular_function	thymidylate synthase activity
H	0005542	molecular_function	folic acid binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005739	cellular_component	mitochondrion
H	0005743	cellular_component	mitochondrial inner membrane
H	0005759	cellular_component	mitochondrial matrix
H	0005829	cellular_component	cytosol
H	0006206	biological_process	pyrimidine nucleobase metabolic process
H	0006231	biological_process	dTMP biosynthetic process
H	0006235	biological_process	dTTP biosynthetic process
H	0006417	biological_process	regulation of translation
H	0007623	biological_process	circadian rhythm
H	0008168	molecular_function	methyltransferase activity
H	0009165	biological_process	nucleotide biosynthetic process
H	0009410	biological_process	response to xenobiotic stimulus
H	0009636	biological_process	response to toxic substance
H	0014070	biological_process	response to organic cyclic compound
H	0016741	molecular_function	transferase activity, transferring one-carbon groups
H	0017148	biological_process	negative regulation of translation
H	0019860	biological_process	uracil metabolic process
H	0032259	biological_process	methylation
H	0032570	biological_process	response to progesterone
H	0033189	biological_process	response to vitamin A
H	0034097	biological_process	response to cytokine
H	0035999	biological_process	tetrahydrofolate interconversion
H	0042803	molecular_function	protein homodimerization activity
H	0045471	biological_process	response to ethanol
H	0046653	biological_process	tetrahydrofolate metabolic process
H	0046683	biological_process	response to organophosphorus
H	0048589	biological_process	developmental growth
H	0051216	biological_process	cartilage development
H	0051384	biological_process	response to glucocorticoid
H	0051593	biological_process	response to folic acid
H	0060574	biological_process	intestinal epithelial cell maturation
H	0071897	biological_process	DNA biosynthetic process
H	0097421	biological_process	liver regeneration
H	1901363	molecular_function	heterocyclic compound binding
H	1990825	molecular_function	sequence-specific mRNA binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue SO4 A 401

Chain	Residue
A	ARG50
A	LEU192
A	CME195
A	ARG215
A	SER216
A	HOH578
B	ARG175
B	ARG176

---

site_id	AC2
Number of Residues	2
Details	binding site for residue SO4 A 402

Chain	Residue
A	ARG64
A	ARG62

---

site_id	AC3
Number of Residues	24
Details	binding site for residue FFO A 403

Chain	Residue
A	PHE80
A	ILE108
A	TRP109
A	ASN112
A	LEU192
A	CME195
A	ASP218
A	LEU221
A	GLY222
A	PHE225
A	ASN226
A	MET311
A	ALA312
A	HOH501
A	HOH504
A	HOH508
A	HOH515
A	HOH517
A	HOH526
A	HOH531
A	HOH542
A	HOH545
A	HOH548
A	HOH557

---

site_id	AC4
Number of Residues	1
Details	binding site for residue CL A 404

Chain	Residue
A	GLY157

---

site_id	AC5
Number of Residues	1
Details	binding site for residue CL A 405

Chain	Residue
A	THR306

---

site_id	AC6
Number of Residues	6
Details	binding site for residue SO4 C 401

Chain	Residue
C	ARG175
C	ARG176
C	HOH571
D	ARG50
D	ARG215
D	SER216

---

site_id	AC7
Number of Residues	8
Details	binding site for residue SO4 C 402

Chain	Residue
C	ARG50
C	LEU192
C	CME195
C	ARG215
C	SER216
C	HOH528
D	ARG175
D	ARG176

---

site_id	AC8
Number of Residues	4
Details	binding site for residue SO4 C 403

Chain	Residue
C	ARG62
C	ARG64
C	HOH560
C	HOH612

---

site_id	AC9
Number of Residues	24
Details	binding site for residue FFO C 404

Chain	Residue
C	PHE80
C	ILE108
C	TRP109
C	ASN112
C	LEU192
C	CME195
C	ASP218
C	LEU221
C	GLY222
C	PHE225
C	ASN226
C	MET311
C	ALA312
C	HOH502
C	HOH517
C	HOH521
C	HOH551
C	HOH552
C	HOH559
C	HOH564
C	HOH585
C	HOH590
C	HOH597
C	HOH608

---

site_id	AD1
Number of Residues	1
Details	binding site for residue CL C 405

Chain	Residue
C	THR306

---

site_id	AD2
Number of Residues	3
Details	binding site for residue SO4 D 401

Chain	Residue
D	ARG62
D	ARG64
D	HOH555

---

site_id	AD3
Number of Residues	3
Details	binding site for residue SO4 D 402

Chain	Residue
D	ARG274
D	HIS304
D	HOH502

---

site_id	AD4
Number of Residues	25
Details	binding site for residue FFO D 403

Chain	Residue
D	HOH510
D	HOH530
D	HOH532
D	HOH535
D	HOH539
D	HOH561
D	HOH569
D	HOH575
D	HOH590
D	HOH622
D	HOH623
D	PHE80
D	ILE108
D	TRP109
D	ASN112
D	LEU192
D	CME195
D	ASP218
D	LEU221
D	GLY222
D	ASN226
D	MET311
D	ALA312
D	GOL404
D	HOH506

---

site_id	AD5
Number of Residues	3
Details	binding site for residue GOL D 404

Chain	Residue
D	FFO403
D	HOH536
D	HOH541

---

site_id	AD6
Number of Residues	2
Details	binding site for residue CL D 405

Chain	Residue
D	THR306
D	HOH536

---

site_id	AD7
Number of Residues	1
Details	binding site for residue CL D 406

Chain	Residue
D	THR125

---

site_id	AD8
Number of Residues	7
Details	binding site for residue SO4 B 401

Chain	Residue
A	ARG175
A	ARG176
B	ARG50
B	CME195
B	ARG215
B	SER216
B	HOH504

---

site_id	AD9
Number of Residues	3
Details	binding site for residue SO4 B 402

Chain	Residue
B	ARG62
B	ARG64
E	LYS266

---

site_id	AE1
Number of Residues	25
Details	binding site for residue FFO B 403

Chain	Residue
B	PHE80
B	TRP109
B	ASN112
B	LEU192
B	CME195
B	ASP218
B	LEU221
B	GLY222
B	PHE225
B	ASN226
B	MET311
B	ALA312
B	HOH502
B	HOH503
B	HOH506
B	HOH507
B	HOH513
B	HOH526
B	HOH534
B	HOH536
B	HOH543
B	HOH548
B	HOH564
B	HOH567
B	HOH603

---

site_id	AE2
Number of Residues	1
Details	binding site for residue CL B 404

Chain	Residue
B	ARG274

---

site_id	AE3
Number of Residues	7
Details	binding site for residue SO4 F 401

Chain	Residue
E	ARG175
E	ARG176
F	ARG50
F	CME195
F	ARG215
F	SER216
F	HOH553

---

site_id	AE4
Number of Residues	2
Details	binding site for residue SO4 F 402

Chain	Residue
F	ARG62
F	ARG64

---

site_id	AE5
Number of Residues	24
Details	binding site for residue FFO F 403

Chain	Residue
F	PHE80
F	ILE108
F	TRP109
F	ASN112
F	LEU192
F	ASP218
F	LEU221
F	GLY222
F	PHE225
F	ASN226
F	MET311
F	ALA312
F	HOH504
F	HOH520
F	HOH531
F	HOH532
F	HOH539
F	HOH542
F	HOH578
F	HOH590
F	HOH591
F	HOH606
F	HOH615
F	HOH635

---

site_id	AE6
Number of Residues	5
Details	binding site for residue SO4 H 401

Chain	Residue
G	ARG175
G	ARG176
H	ARG50
H	ARG215
H	SER216

---

site_id	AE7
Number of Residues	6
Details	binding site for residue SO4 H 402

Chain	Residue
B	ARG46
B	THR53
B	LEU259
H	ARG46
H	HOH502
H	HOH512

---

site_id	AE8
Number of Residues	20
Details	binding site for residue FFO H 403

Chain	Residue
H	PHE80
H	ILE108
H	TRP109
H	ASN112
H	TYR135
H	CME195
H	ASP218
H	LEU221
H	GLY222
H	PHE225
H	MET311
H	ALA312
H	HOH501
H	HOH503
H	HOH509
H	HOH527
H	HOH535
H	HOH542
H	HOH579
H	HOH588

---

site_id	AE9
Number of Residues	7
Details	binding site for residue SO4 E 401

Chain	Residue
E	ARG50
E	LEU192
E	ARG215
E	SER216
E	HOH520
F	ARG175
F	ARG176

---

site_id	AF1
Number of Residues	3
Details	binding site for residue SO4 E 402

Chain	Residue
E	ARG274
E	HIS304
E	HOH602

---

site_id	AF2
Number of Residues	21
Details	binding site for residue FFO E 403

Chain	Residue
E	PHE80
E	ILE108
E	TRP109
E	ASN112
E	CME195
E	ASP218
E	LEU221
E	GLY222
E	PHE225
E	MET311
E	ALA312
E	HOH503
E	HOH505
E	HOH510
E	HOH513
E	HOH547
E	HOH551
E	HOH552
E	HOH573
E	HOH611
E	HOH629

---

site_id	AF3
Number of Residues	1
Details	binding site for residue CL E 404

Chain	Residue
E	ARG147

---

site_id	AF4
Number of Residues	8
Details	binding site for residue SO4 G 401

Chain	Residue
G	ARG50
G	LEU192
G	CME195
G	ARG215
G	SER216
G	HOH530
H	ARG175
H	ARG176

---

site_id	AF5
Number of Residues	4
Details	binding site for residue SO4 G 402

Chain	Residue
G	GLU272
G	ARG274
G	HIS304
G	HOH593

---

site_id	AF6
Number of Residues	23
Details	binding site for residue FFO G 403

Chain	Residue
G	PHE80
G	ILE108
G	TRP109
G	ASN112
G	LEU192
G	ASP218
G	LEU221
G	GLY222
G	PHE225
G	ASN226
G	MET311
G	ALA312
G	HOH502
G	HOH507
G	HOH537
G	HOH539
G	HOH544
G	HOH549
G	HOH554
G	HOH558
G	HOH565
G	HOH598
G	HOH616

---

site_id	AF7
Number of Residues	2
Details	binding site for residue CL G 404

Chain	Residue
G	ARG147
G	ASP152

---

Functional Information from PROSITE/UniProt

site_id	PS00091
Number of Residues	29
Details	THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV

Chain	Residue	Details
E	ARG175-VAL203
A	ARG175-VAL203

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P0A884

Chain	Residue	Details
E	CME195
G	CME195
D	CME195
B	CME195
F	CME195
H	CME195

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: in other chain => ECO:0000250|UniProtKB:P0A884

Chain	Residue	Details
E	ARG50
F	ASN226
H	ARG50
H	ASN226
E	ASN226
G	ARG50
G	ASN226
D	ARG50
D	ASN226
B	ARG50
B	ASN226
F	ARG50

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P45352

Chain	Residue	Details
E	ARG175
G	ARG175
D	ARG175
B	ARG175
F	ARG175
H	ARG175

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: in other chain => ECO:0000250|UniProtKB:P45352

Chain	Residue	Details
E	CME195
B	CME195
B	ARG215
B	HIS256
F	CME195
F	ARG215
F	HIS256
H	CME195
H	ARG215
H	HIS256
E	ARG215
E	HIS256
G	CME195
G	ARG215
G	HIS256
D	CME195
D	ARG215
D	HIS256

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P0A884

Chain	Residue	Details
E	ASP218
F	ALA312
H	ASP218
H	ALA312
E	ALA312
G	ASP218
G	ALA312
D	ASP218
D	ALA312
B	ASP218
B	ALA312
F	ASP218

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163

Chain	Residue	Details
E	SER114
G	SER114
D	SER114
B	SER114
F	SER114
H	SER114

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
E	LYS287
D	LYS292
D	LYS308
B	LYS287
B	LYS292
B	LYS308
F	LYS287
F	LYS292
F	LYS308
H	LYS287
H	LYS292
H	LYS308
E	LYS292
E	LYS308
G	LYS287
G	LYS292
G	LYS308
D	LYS287

---