6QVE

NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0003725	molecular_function	double-stranded RNA binding
A	0003924	molecular_function	GTPase activity
A	0005198	molecular_function	structural molecule activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0005881	cellular_component	cytoplasmic microtubule
A	0007017	biological_process	microtubule-based process
A	0015630	cellular_component	microtubule cytoskeleton
A	0016787	molecular_function	hydrolase activity
A	0030705	biological_process	cytoskeleton-dependent intracellular transport
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0051301	biological_process	cell division
A	0071353	biological_process	cellular response to interleukin-4
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000278	biological_process	mitotic cell cycle
C	0003725	molecular_function	double-stranded RNA binding
C	0003924	molecular_function	GTPase activity
C	0005198	molecular_function	structural molecule activity
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0005881	cellular_component	cytoplasmic microtubule
C	0007017	biological_process	microtubule-based process
C	0015630	cellular_component	microtubule cytoskeleton
C	0016787	molecular_function	hydrolase activity
C	0030705	biological_process	cytoskeleton-dependent intracellular transport
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0051301	biological_process	cell division
C	0071353	biological_process	cellular response to interleukin-4
G	0000226	biological_process	microtubule cytoskeleton organization
G	0000278	biological_process	mitotic cell cycle
G	0003924	molecular_function	GTPase activity
G	0005198	molecular_function	structural molecule activity
G	0005200	molecular_function	structural constituent of cytoskeleton
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005641	cellular_component	nuclear envelope lumen
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005874	cellular_component	microtubule
G	0007017	biological_process	microtubule-based process
G	0015630	cellular_component	microtubule cytoskeleton
G	0019904	molecular_function	protein domain specific binding
G	0030705	biological_process	cytoskeleton-dependent intracellular transport
G	0031625	molecular_function	ubiquitin protein ligase binding
G	0032794	molecular_function	GTPase activating protein binding
G	0032991	cellular_component	protein-containing complex
G	0035578	cellular_component	azurophil granule lumen
G	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
G	0042267	biological_process	natural killer cell mediated cytotoxicity
G	0042288	molecular_function	MHC class I protein binding
G	0044297	cellular_component	cell body
G	0044877	molecular_function	protein-containing complex binding
G	0045121	cellular_component	membrane raft
G	0045171	cellular_component	intercellular bridge
G	0046872	molecular_function	metal ion binding
G	0050807	biological_process	regulation of synapse organization
G	0051225	biological_process	spindle assembly
G	0051301	biological_process	cell division
G	0070062	cellular_component	extracellular exosome
G	0071895	biological_process	odontoblast differentiation
G	0072686	cellular_component	mitotic spindle
H	0000226	biological_process	microtubule cytoskeleton organization
H	0000278	biological_process	mitotic cell cycle
H	0003924	molecular_function	GTPase activity
H	0005198	molecular_function	structural molecule activity
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005515	molecular_function	protein binding
H	0005525	molecular_function	GTP binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005641	cellular_component	nuclear envelope lumen
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0005874	cellular_component	microtubule
H	0007017	biological_process	microtubule-based process
H	0015630	cellular_component	microtubule cytoskeleton
H	0019904	molecular_function	protein domain specific binding
H	0030705	biological_process	cytoskeleton-dependent intracellular transport
H	0031625	molecular_function	ubiquitin protein ligase binding
H	0032794	molecular_function	GTPase activating protein binding
H	0032991	cellular_component	protein-containing complex
H	0035578	cellular_component	azurophil granule lumen
H	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
H	0042267	biological_process	natural killer cell mediated cytotoxicity
H	0042288	molecular_function	MHC class I protein binding
H	0044297	cellular_component	cell body
H	0044877	molecular_function	protein-containing complex binding
H	0045121	cellular_component	membrane raft
H	0045171	cellular_component	intercellular bridge
H	0046872	molecular_function	metal ion binding
H	0050807	biological_process	regulation of synapse organization
H	0051225	biological_process	spindle assembly
H	0051301	biological_process	cell division
H	0070062	cellular_component	extracellular exosome
H	0071895	biological_process	odontoblast differentiation
H	0072686	cellular_component	mitotic spindle
W	0005516	molecular_function	calmodulin binding
W	0008017	molecular_function	microtubule binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	binding site for residue GTP C 501

Chain	Residue
C	GLY10
C	GLY143
C	THR145
C	GLY146
C	ASN206
C	TYR224
C	ASN228
C	MG502
G	LYS254
C	GLN11
C	ALA12
C	GLN15
C	GLU71
C	ASP98
C	ALA100
C	ASN101
C	SER140

---

site_id	AC2
Number of Residues	2
Details	binding site for residue MG C 502

Chain	Residue
C	GLU71
C	GTP501

---

site_id	AC3
Number of Residues	20
Details	binding site for residue GTP A 501

Chain	Residue
A	GLY10
A	GLN11
A	ALA12
A	GLN15
A	ILE16
A	ASP98
A	ALA100
A	ASN101
A	SER140
A	GLY143
A	THR145
A	GLY146
A	THR179
A	ASN206
A	TYR224
A	ASN228
A	MG502
H	GLN247
H	LEU248
H	LYS254

---

site_id	AC4
Number of Residues	3
Details	binding site for residue MG A 502

Chain	Residue
A	GLU71
A	GTP501
H	LYS254

---

site_id	AC5
Number of Residues	11
Details	binding site for residue GDP H 501

Chain	Residue
H	GLY10
H	GLN11
H	CYS12
H	GLN15
H	GLY143
H	GLY144
H	THR145
H	GLY146
H	ASN206
H	TYR224
H	ASN228

---

site_id	AC6
Number of Residues	12
Details	binding site for residue TA1 H 502

Chain	Residue
H	GLU22
H	VAL23
H	ASP26
H	GLU27
H	HIS229
H	ALA233
H	SER236
H	THR276
H	ARG278
H	GLN281
H	ARG369
H	LEU371

---

site_id	AC7
Number of Residues	11
Details	binding site for residue GDP G 501

Chain	Residue
G	GLY10
G	GLN11
G	CYS12
G	GLN15
G	GLY143
G	GLY144
G	THR145
G	GLY146
G	ASN206
G	TYR224
G	ASN228

---

site_id	AC8
Number of Residues	10
Details	binding site for residue TA1 G 502

Chain	Residue
G	VAL23
G	ASP26
G	GLU27
G	HIS229
G	ALA233
G	THR276
G	ARG278
G	GLN281
G	ARG369
G	LEU371

---

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
C	GLY142-GLY148
H	GLY142-GLY148

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
H	MET1-ILE4

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q13509

Chain	Residue	Details
H	GLN11
G	GLY144
G	THR145
G	GLY146
G	ASN206
G	ASN228
H	SER140
H	GLY144
H	THR145
H	GLY146
H	ASN206
H	ASN228
G	GLN11
G	SER140

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
H	GLU71
A	GLU71
A	SER140
A	GLY144
A	THR145
A	THR179
A	ASN206
A	ASN228
G	GLU71
C	SER140
C	GLY144
C	THR145
C	THR179
C	ASN206
C	ASN228
A	GLN11

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
H	SER40
G	SER40

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
H	THR57
H	THR287
H	THR292
G	THR57
G	THR287
G	THR292

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
H	LYS60
G	LYS60
A	SER48
A	SER232

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphoserine; by CDK1 => ECO:0000269|PubMed:16371510

Chain	Residue	Details
H	SER174
G	SER174

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Omega-N-methylarginine => ECO:0000269|Ref.9

Chain	Residue	Details
H	ARG320
G	ARG320

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000269|PubMed:28576883

Chain	Residue	Details
H	GLU444
H	GLU451
G	GLU444
G	GLU451

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000269|PubMed:32747782

Chain	Residue	Details
H	GLU448
H	GLU449
G	GLU448
G	GLU449

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: 5-glutamyl glycine => ECO:0000269|PubMed:28576883

Chain	Residue	Details
H	GLU452
H	GLU453
G	GLU452
G	GLU453

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate

Chain	Residue	Details
H	LYS60
G	LYS60

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
C	LYS326
H	LYS326
C	LYS370
G	LYS326
A	LYS370

---