6QUQ
Crystal structure of glutathionylated glycolytic glyceraldehyde-3- phosphate dehydrogenase from Arabidopsis thaliana (AtGAPC1)
Functional Information from GO Data
Chain | GOid | namespace | contents |
O | 0000325 | cellular_component | plant-type vacuole |
O | 0003677 | molecular_function | DNA binding |
O | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
O | 0005507 | molecular_function | copper ion binding |
O | 0005515 | molecular_function | protein binding |
O | 0005634 | cellular_component | nucleus |
O | 0005737 | cellular_component | cytoplasm |
O | 0005739 | cellular_component | mitochondrion |
O | 0005740 | cellular_component | mitochondrial envelope |
O | 0005794 | cellular_component | Golgi apparatus |
O | 0005829 | cellular_component | cytosol |
O | 0005886 | cellular_component | plasma membrane |
O | 0006006 | biological_process | glucose metabolic process |
O | 0006094 | biological_process | gluconeogenesis |
O | 0006096 | biological_process | glycolytic process |
O | 0006979 | biological_process | response to oxidative stress |
O | 0008886 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity |
O | 0009408 | biological_process | response to heat |
O | 0009507 | cellular_component | chloroplast |
O | 0009744 | biological_process | response to sucrose |
O | 0010154 | biological_process | fruit development |
O | 0016491 | molecular_function | oxidoreductase activity |
O | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
O | 0042542 | biological_process | response to hydrogen peroxide |
O | 0045893 | biological_process | positive regulation of DNA-templated transcription |
O | 0048046 | cellular_component | apoplast |
O | 0048316 | biological_process | seed development |
O | 0050661 | molecular_function | NADP binding |
O | 0051287 | molecular_function | NAD binding |
O | 0051775 | biological_process | response to redox state |
O | 1901149 | molecular_function | salicylic acid binding |
R | 0000325 | cellular_component | plant-type vacuole |
R | 0003677 | molecular_function | DNA binding |
R | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
R | 0005507 | molecular_function | copper ion binding |
R | 0005515 | molecular_function | protein binding |
R | 0005634 | cellular_component | nucleus |
R | 0005737 | cellular_component | cytoplasm |
R | 0005739 | cellular_component | mitochondrion |
R | 0005740 | cellular_component | mitochondrial envelope |
R | 0005794 | cellular_component | Golgi apparatus |
R | 0005829 | cellular_component | cytosol |
R | 0005886 | cellular_component | plasma membrane |
R | 0006006 | biological_process | glucose metabolic process |
R | 0006094 | biological_process | gluconeogenesis |
R | 0006096 | biological_process | glycolytic process |
R | 0006979 | biological_process | response to oxidative stress |
R | 0008886 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity |
R | 0009408 | biological_process | response to heat |
R | 0009507 | cellular_component | chloroplast |
R | 0009744 | biological_process | response to sucrose |
R | 0010154 | biological_process | fruit development |
R | 0016491 | molecular_function | oxidoreductase activity |
R | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
R | 0042542 | biological_process | response to hydrogen peroxide |
R | 0045893 | biological_process | positive regulation of DNA-templated transcription |
R | 0048046 | cellular_component | apoplast |
R | 0048316 | biological_process | seed development |
R | 0050661 | molecular_function | NADP binding |
R | 0051287 | molecular_function | NAD binding |
R | 0051775 | biological_process | response to redox state |
R | 1901149 | molecular_function | salicylic acid binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 22 |
Details | binding site for residue NAD O 401 |
Chain | Residue |
O | ASN6 |
O | PHE34 |
O | ARG77 |
O | SER95 |
O | THR96 |
O | GLY97 |
O | PHE99 |
O | SER119 |
O | ALA120 |
O | ALA180 |
O | ASN313 |
O | GLY7 |
O | TYR317 |
O | SO4402 |
O | GSH404 |
O | PHE8 |
O | GLY9 |
O | ARG10 |
O | ILE11 |
O | ASN31 |
O | ASP32 |
O | PRO33 |
site_id | AC2 |
Number of Residues | 5 |
Details | binding site for residue SO4 O 402 |
Chain | Residue |
O | THR179 |
O | THR181 |
O | ARG231 |
O | NAD401 |
O | GSH404 |
site_id | AC3 |
Number of Residues | 6 |
Details | binding site for residue SO4 O 403 |
Chain | Residue |
O | SER148 |
O | THR150 |
O | THR208 |
O | GLY209 |
O | ALA210 |
O | GSH404 |
site_id | AC4 |
Number of Residues | 15 |
Details | binding site for residue GSH O 404 |
Chain | Residue |
O | ALA120 |
O | PRO121 |
O | SER148 |
O | CYS149 |
O | THR150 |
O | THR174 |
O | HIS176 |
O | SER206 |
O | SER207 |
O | THR208 |
O | SER229 |
O | ARG231 |
O | NAD401 |
O | SO4402 |
O | SO4403 |
site_id | AC5 |
Number of Residues | 6 |
Details | binding site for residue SO4 O 405 |
Chain | Residue |
O | SER200 |
O | SER200 |
O | PRO233 |
R | SER200 |
R | SER200 |
R | PHE201 |
site_id | AC6 |
Number of Residues | 24 |
Details | binding site for residue NAD R 401 |
Chain | Residue |
R | ASN6 |
R | GLY7 |
R | PHE8 |
R | GLY9 |
R | ARG10 |
R | ILE11 |
R | ASN31 |
R | ASP32 |
R | PRO33 |
R | PHE34 |
R | ILE35 |
R | ARG77 |
R | SER95 |
R | THR96 |
R | GLY97 |
R | SER119 |
R | ALA120 |
R | ALA180 |
R | ASN313 |
R | GLU314 |
R | TYR317 |
R | SO4402 |
R | GSH404 |
R | HOH505 |
site_id | AC7 |
Number of Residues | 5 |
Details | binding site for residue SO4 R 402 |
Chain | Residue |
R | THR179 |
R | THR181 |
R | ARG231 |
R | NAD401 |
R | GSH404 |
site_id | AC8 |
Number of Residues | 7 |
Details | binding site for residue SO4 R 403 |
Chain | Residue |
R | PRO121 |
R | SER148 |
R | THR150 |
R | THR208 |
R | GLY209 |
R | ALA210 |
R | GSH404 |
site_id | AC9 |
Number of Residues | 2 |
Details | binding site for residue SO4 R 405 |
Chain | Residue |
R | ASN287 |
R | TRP315 |
site_id | AD1 |
Number of Residues | 2 |
Details | binding site for residue SO4 R 406 |
Chain | Residue |
R | LYS268 |
R | ARG288 |
site_id | AD2 |
Number of Residues | 17 |
Details | binding site for Di-peptide GSH R 404 and CYS R 149 |
Chain | Residue |
R | CYS153 |
R | HIS176 |
R | SER206 |
R | SER207 |
R | THR208 |
R | SER229 |
R | ARG231 |
R | TYR311 |
R | TYR317 |
R | NAD401 |
R | SO4402 |
R | SO4403 |
R | PRO121 |
R | SER148 |
R | THR150 |
R | THR151 |
R | ASN152 |
Functional Information from PROSITE/UniProt
site_id | PS00071 |
Number of Residues | 8 |
Details | GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL |
Chain | Residue | Details |
O | ALA147-LEU154 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 2 |
Details | ACT_SITE: Nucleophile => ECO:0000269|Ref.18 |
Chain | Residue | Details |
O | CYS149 | |
R | CYS149 |
site_id | SWS_FT_FI2 |
Number of Residues | 16 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P00362 |
Chain | Residue | Details |
O | ARG10 | |
R | ASP32 | |
R | ARG77 | |
R | SER148 | |
R | THR179 | |
R | THR208 | |
R | ARG231 | |
R | ASN313 | |
O | ASP32 | |
O | ARG77 | |
O | SER148 | |
O | THR179 | |
O | THR208 | |
O | ARG231 | |
O | ASN313 | |
R | ARG10 |
site_id | SWS_FT_FI3 |
Number of Residues | 2 |
Details | SITE: Activates thiol group during catalysis => ECO:0000250|UniProtKB:P00362 |
Chain | Residue | Details |
O | HIS176 | |
R | HIS176 |
site_id | SWS_FT_FI4 |
Number of Residues | 2 |
Details | MOD_RES: S-nitrosocysteine; transient; alternate => ECO:0000250|UniProtKB:Q9FX54 |
Chain | Residue | Details |
O | CYS149 | |
R | CYS149 |
site_id | SWS_FT_FI5 |
Number of Residues | 2 |
Details | MOD_RES: S-nitrosocysteine; transient => ECO:0000250|UniProtKB:Q9FX54 |
Chain | Residue | Details |
O | CYS153 | |
R | CYS153 |