6QU5

Crystal Structure of Phosphofructokinase from Trypanosoma brucei in complex with an allosteric inhibitor ctcb12

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003824	molecular_function	catalytic activity
A	0003872	molecular_function	6-phosphofructokinase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006002	biological_process	fructose 6-phosphate metabolic process
A	0006096	biological_process	glycolytic process
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0020015	cellular_component	glycosome
A	0042301	molecular_function	phosphate ion binding
A	0046872	molecular_function	metal ion binding
A	0061615	biological_process	glycolytic process through fructose-6-phosphate
B	0000166	molecular_function	nucleotide binding
B	0003824	molecular_function	catalytic activity
B	0003872	molecular_function	6-phosphofructokinase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006002	biological_process	fructose 6-phosphate metabolic process
B	0006096	biological_process	glycolytic process
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0020015	cellular_component	glycosome
B	0042301	molecular_function	phosphate ion binding
B	0046872	molecular_function	metal ion binding
B	0061615	biological_process	glycolytic process through fructose-6-phosphate
C	0000166	molecular_function	nucleotide binding
C	0003824	molecular_function	catalytic activity
C	0003872	molecular_function	6-phosphofructokinase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006002	biological_process	fructose 6-phosphate metabolic process
C	0006096	biological_process	glycolytic process
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0020015	cellular_component	glycosome
C	0042301	molecular_function	phosphate ion binding
C	0046872	molecular_function	metal ion binding
C	0061615	biological_process	glycolytic process through fructose-6-phosphate
D	0000166	molecular_function	nucleotide binding
D	0003824	molecular_function	catalytic activity
D	0003872	molecular_function	6-phosphofructokinase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006002	biological_process	fructose 6-phosphate metabolic process
D	0006096	biological_process	glycolytic process
D	0016301	molecular_function	kinase activity
D	0016740	molecular_function	transferase activity
D	0020015	cellular_component	glycosome
D	0042301	molecular_function	phosphate ion binding
D	0046872	molecular_function	metal ion binding
D	0061615	biological_process	glycolytic process through fructose-6-phosphate
E	0000166	molecular_function	nucleotide binding
E	0003824	molecular_function	catalytic activity
E	0003872	molecular_function	6-phosphofructokinase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0006002	biological_process	fructose 6-phosphate metabolic process
E	0006096	biological_process	glycolytic process
E	0016301	molecular_function	kinase activity
E	0016740	molecular_function	transferase activity
E	0020015	cellular_component	glycosome
E	0042301	molecular_function	phosphate ion binding
E	0046872	molecular_function	metal ion binding
E	0061615	biological_process	glycolytic process through fructose-6-phosphate
F	0000166	molecular_function	nucleotide binding
F	0003824	molecular_function	catalytic activity
F	0003872	molecular_function	6-phosphofructokinase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0006002	biological_process	fructose 6-phosphate metabolic process
F	0006096	biological_process	glycolytic process
F	0016301	molecular_function	kinase activity
F	0016740	molecular_function	transferase activity
F	0020015	cellular_component	glycosome
F	0042301	molecular_function	phosphate ion binding
F	0046872	molecular_function	metal ion binding
F	0061615	biological_process	glycolytic process through fructose-6-phosphate
G	0000166	molecular_function	nucleotide binding
G	0003824	molecular_function	catalytic activity
G	0003872	molecular_function	6-phosphofructokinase activity
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0006002	biological_process	fructose 6-phosphate metabolic process
G	0006096	biological_process	glycolytic process
G	0016301	molecular_function	kinase activity
G	0016740	molecular_function	transferase activity
G	0020015	cellular_component	glycosome
G	0042301	molecular_function	phosphate ion binding
G	0046872	molecular_function	metal ion binding
G	0061615	biological_process	glycolytic process through fructose-6-phosphate
H	0000166	molecular_function	nucleotide binding
H	0003824	molecular_function	catalytic activity
H	0003872	molecular_function	6-phosphofructokinase activity
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0006002	biological_process	fructose 6-phosphate metabolic process
H	0006096	biological_process	glycolytic process
H	0016301	molecular_function	kinase activity
H	0016740	molecular_function	transferase activity
H	0020015	cellular_component	glycosome
H	0042301	molecular_function	phosphate ion binding
H	0046872	molecular_function	metal ion binding
H	0061615	biological_process	glycolytic process through fructose-6-phosphate

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	binding site for residue CIT A 501

Chain	Residue
A	GLY106
A	GLY107
A	ARG173
A	GLY174
A	GLY198
A	ASP199
A	GLY200
A	THR201
A	LYS226

---

site_id	AC2
Number of Residues	6
Details	binding site for residue GOL A 502

Chain	Residue
A	ILE292
A	CYS293
A	ASN298
A	TRP444
A	THR451
A	VAL452

---

site_id	AC3
Number of Residues	8
Details	binding site for residue JJ8 A 503

Chain	Residue
A	HIS17
A	GLY197
A	GLY198
A	ASP199
A	LEU232
A	ASP275
A	ALA430
A	ARG434

---

site_id	AC4
Number of Residues	10
Details	binding site for residue CIT B 501

Chain	Residue
B	GLY106
B	GLY107
B	ARG173
B	GLY198
B	ASP199
B	GLY200
B	THR201
B	LYS226
B	SER341
B	ASN343

---

site_id	AC5
Number of Residues	5
Details	binding site for residue GOL B 502

Chain	Residue
B	ILE292
B	CYS293
B	ASN298
B	ARG448
B	THR451

---

site_id	AC6
Number of Residues	11
Details	binding site for residue JJ8 B 503

Chain	Residue
B	GLY197
B	GLY198
B	ASP199
B	GLN202
B	PRO225
B	ASP231
B	LEU232
B	ASP275
B	ALA430
B	VAL433
B	ARG434

---

site_id	AC7
Number of Residues	9
Details	binding site for residue CIT C 501

Chain	Residue
C	GLY106
C	GLY107
C	ARG173
C	GLY174
C	GLY198
C	ASP199
C	GLY200
C	THR201
C	LYS226

---

site_id	AC8
Number of Residues	9
Details	binding site for residue CIT D 501

Chain	Residue
D	GLY106
D	GLY107
D	ARG173
D	GLY174
D	GLY198
D	ASP199
D	GLY200
D	THR201
D	LYS226

---

site_id	AC9
Number of Residues	8
Details	binding site for residue CIT E 501

Chain	Residue
E	GLY106
E	ARG173
E	GLY174
E	GLY198
E	ASP199
E	GLY200
E	THR201
E	LYS226

---

site_id	AD1
Number of Residues	8
Details	binding site for residue CIT F 501

Chain	Residue
F	GLY106
F	GLY107
F	ARG173
F	GLY198
F	ASP199
F	GLY200
F	THR201
F	LYS226

---

site_id	AD2
Number of Residues	8
Details	binding site for residue CIT G 501

Chain	Residue
G	GLY106
G	ARG173
G	GLY174
G	GLY198
G	ASP199
G	GLY200
G	THR201
G	LYS226

---

site_id	AD3
Number of Residues	8
Details	binding site for Di-peptide CIT H 501 and ARG H 173

Chain	Residue
H	GLY107
H	SER172
H	GLY174
H	GLY198
H	ASP199
H	GLY200
H	THR201
H	LYS226

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_03186","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	46
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03186","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"19084537","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	72
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03186","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19084537","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Site: {"description":"Important for substrate specificity; cannot use PPi as phosphoryl donor","evidences":[{"source":"HAMAP-Rule","id":"MF_03186","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---