6QSN
Crystal structure of Yellow fever virus polymerase NS5A
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003968 | molecular_function | RNA-dependent RNA polymerase activity |
| A | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| A | 0004483 | molecular_function | mRNA (nucleoside-2'-O-)-methyltransferase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0032259 | biological_process | methylation |
| A | 0039694 | biological_process | viral RNA genome replication |
| B | 0003968 | molecular_function | RNA-dependent RNA polymerase activity |
| B | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| B | 0004483 | molecular_function | mRNA (nucleoside-2'-O-)-methyltransferase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0032259 | biological_process | methylation |
| B | 0039694 | biological_process | viral RNA genome replication |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 14 |
| Details | binding site for residue SAH A 1001 |
| Chain | Residue |
| A | SER56 |
| A | LEU105 |
| A | HIS110 |
| A | GLU111 |
| A | ILE132 |
| A | ASP146 |
| A | GLY81 |
| A | CYS82 |
| A | GLY83 |
| A | ARG84 |
| A | GLY85 |
| A | GLY86 |
| A | TRP87 |
| A | THR104 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 A 1002 |
| Chain | Residue |
| A | ARG37 |
| A | ARG41 |
| A | SER56 |
| A | ARG57 |
| A | ARG84 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 A 1003 |
| Chain | Residue |
| A | TRP381 |
| A | ARG384 |
| A | THR652 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 A 1004 |
| Chain | Residue |
| A | ARG733 |
| A | THR798 |
| A | TRP799 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 1005 |
| Chain | Residue |
| A | GLU439 |
| A | HIS443 |
| A | CYS448 |
| A | CYS451 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 1006 |
| Chain | Residue |
| A | HIS716 |
| A | HIS718 |
| A | CYS732 |
| A | CYS851 |
| site_id | AC7 |
| Number of Residues | 12 |
| Details | binding site for residue SAH B 1001 |
| Chain | Residue |
| B | SER56 |
| B | GLY81 |
| B | CYS82 |
| B | ARG84 |
| B | THR104 |
| B | LEU105 |
| B | THR130 |
| B | ASP131 |
| B | ILE132 |
| B | HIS133 |
| B | ASP146 |
| B | ILE147 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 B 1004 |
| Chain | Residue |
| B | ARG302 |
| B | ARG363 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 B 1005 |
| Chain | Residue |
| B | ARG733 |
| B | ARG741 |
| B | THR798 |
| site_id | AD1 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 1006 |
| Chain | Residue |
| B | GLU439 |
| B | HIS443 |
| B | CYS448 |
| B | CYS451 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 1007 |
| Chain | Residue |
| B | HIS716 |
| B | HIS718 |
| B | CYS732 |
| B | CYS851 |
| site_id | AD3 |
| Number of Residues | 8 |
| Details | binding site for Di-peptide SO4 B 1002 and ARG B 384 |
| Chain | Residue |
| B | ARG380 |
| B | TRP381 |
| B | LEU382 |
| B | PHE383 |
| B | HIS385 |
| B | LEU386 |
| B | TYR557 |
| B | THR652 |
| site_id | AD4 |
| Number of Residues | 11 |
| Details | binding site for Di-peptide SO4 B 1003 and ARG B 84 |
| Chain | Residue |
| B | ARG37 |
| B | ARG41 |
| B | VAL55 |
| B | ARG57 |
| B | CYS82 |
| B | GLY83 |
| B | GLY85 |
| B | GLU111 |
| B | PRO113 |
| B | MET114 |
| B | SAH1001 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | ACT_SITE: For 2'-O-MTase activity => ECO:0000250|UniProtKB:Q6YMS4 |
| Chain | Residue | Details |
| A | LYS61 | |
| A | ASP146 | |
| A | LYS182 | |
| A | GLU218 | |
| B | LYS61 | |
| B | ASP146 | |
| B | LYS182 | |
| B | GLU218 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 18 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 |
| Chain | Residue | Details |
| A | ILE132 | |
| A | ILE147 | |
| A | TYR220 | |
| B | SER56 | |
| B | GLY86 | |
| B | TRP87 | |
| B | THR104 | |
| B | LEU105 | |
| B | ASP131 | |
| B | ILE132 | |
| B | ILE147 | |
| B | TYR220 | |
| A | SER56 | |
| A | GLY86 | |
| A | TRP87 | |
| A | THR104 | |
| A | LEU105 | |
| A | ASP131 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 14 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P14335 |
| Chain | Residue | Details |
| A | CYS448 | |
| A | CYS451 | |
| A | HIS716 | |
| A | CYS732 | |
| A | CYS851 | |
| B | GLU439 | |
| B | HIS443 | |
| B | CYS448 | |
| B | CYS451 | |
| B | HIS716 | |
| B | CYS732 | |
| B | CYS851 | |
| A | GLU439 | |
| A | HIS443 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:19101564 |
| Chain | Residue | Details |
| A | LYS28 | |
| A | SER150 | |
| A | ARG213 | |
| A | SER215 | |
| B | LYS13 | |
| B | ASN17 | |
| B | LYS28 | |
| B | SER150 | |
| B | ARG213 | |
| B | SER215 | |
| A | LYS13 | |
| A | ASN17 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | SITE: mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:19101564 |
| Chain | Residue | Details |
| A | LEU16 | |
| A | LEU19 | |
| B | LEU16 | |
| B | LEU19 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 |
| Chain | Residue | Details |
| A | PHE24 | |
| B | PHE24 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | SITE: Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 |
| Chain | Residue | Details |
| A | LYS61 | |
| A | LYS182 | |
| A | GLU218 | |
| B | LYS61 | |
| B | LYS182 | |
| B | GLU218 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | SITE: Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:18757072 |
| Chain | Residue | Details |
| A | ASP146 | |
| B | ASP146 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:18757072 |
| Chain | Residue | Details |
| A | SER56 | |
| B | SER56 |
