6QSN

Crystal structure of Yellow fever virus polymerase NS5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	108.285, 142.114, 152.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.011 - 3.004
R-factor	0.2232
Rwork	0.221
R-free	0.27060
RMSD bond length	0.002
RMSD bond angle	0.524
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.011	3.110
High resolution limit [Å]	3.004	3.004
Rpim	0.081	0.687
Number of reflections	47148
<I/σ(I)>	9.91
Completeness [%]	99.2
Redundancy	8.3
CC(1/2)	0.994	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	80 mM Tris pH=8.5, 1.92M (NH4)2SO4, 20% (v/v) glycerol