6PK4
cryoEM structure of the substrate-bound human CTP synthase 2 filament
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003883 | molecular_function | CTP synthase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006220 | biological_process | pyrimidine nucleotide metabolic process |
| A | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| A | 0006241 | biological_process | CTP biosynthetic process |
| A | 0006541 | biological_process | glutamine metabolic process |
| A | 0016874 | molecular_function | ligase activity |
| A | 0019637 | biological_process | organophosphate metabolic process |
| A | 0019856 | biological_process | pyrimidine nucleobase biosynthetic process |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0044210 | biological_process | 'de novo' CTP biosynthetic process |
| A | 0097268 | cellular_component | cytoophidium |
| B | 0003883 | molecular_function | CTP synthase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006220 | biological_process | pyrimidine nucleotide metabolic process |
| B | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| B | 0006241 | biological_process | CTP biosynthetic process |
| B | 0006541 | biological_process | glutamine metabolic process |
| B | 0016874 | molecular_function | ligase activity |
| B | 0019637 | biological_process | organophosphate metabolic process |
| B | 0019856 | biological_process | pyrimidine nucleobase biosynthetic process |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0044210 | biological_process | 'de novo' CTP biosynthetic process |
| B | 0097268 | cellular_component | cytoophidium |
| C | 0003883 | molecular_function | CTP synthase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006220 | biological_process | pyrimidine nucleotide metabolic process |
| C | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| C | 0006241 | biological_process | CTP biosynthetic process |
| C | 0006541 | biological_process | glutamine metabolic process |
| C | 0016874 | molecular_function | ligase activity |
| C | 0019637 | biological_process | organophosphate metabolic process |
| C | 0019856 | biological_process | pyrimidine nucleobase biosynthetic process |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0044210 | biological_process | 'de novo' CTP biosynthetic process |
| C | 0097268 | cellular_component | cytoophidium |
| D | 0003883 | molecular_function | CTP synthase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006220 | biological_process | pyrimidine nucleotide metabolic process |
| D | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| D | 0006241 | biological_process | CTP biosynthetic process |
| D | 0006541 | biological_process | glutamine metabolic process |
| D | 0016874 | molecular_function | ligase activity |
| D | 0019637 | biological_process | organophosphate metabolic process |
| D | 0019856 | biological_process | pyrimidine nucleobase biosynthetic process |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0044210 | biological_process | 'de novo' CTP biosynthetic process |
| D | 0097268 | cellular_component | cytoophidium |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 12 |
| Details | binding site for residue UTP A 601 |
| Chain | Residue |
| A | SER12 |
| B | LYS195 |
| B | LYS229 |
| C | GLN112 |
| A | LYS38 |
| A | TYR42 |
| A | HIS55 |
| A | ASP70 |
| A | GLY149 |
| A | ATP602 |
| B | LYS193 |
| B | THR194 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | binding site for residue ATP A 602 |
| Chain | Residue |
| A | LYS16 |
| A | GLY17 |
| A | ILE18 |
| A | LYS38 |
| A | ASP70 |
| A | GLU146 |
| A | ARG217 |
| A | VAL253 |
| A | ASP312 |
| A | UTP601 |
| B | THR189 |
| site_id | AC3 |
| Number of Residues | 11 |
| Details | binding site for residue ATP B 602 |
| Chain | Residue |
| A | THR189 |
| B | LYS16 |
| B | GLY17 |
| B | ILE18 |
| B | LYS38 |
| B | ASP70 |
| B | GLU146 |
| B | ARG217 |
| B | VAL253 |
| B | ASP312 |
| B | UTP601 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | binding site for residue ATP C 602 |
| Chain | Residue |
| C | LYS16 |
| C | GLY17 |
| C | ILE18 |
| C | LYS38 |
| C | ASP70 |
| C | GLU146 |
| C | ARG217 |
| C | VAL253 |
| C | ASP312 |
| C | UTP601 |
| D | THR189 |
| site_id | AC5 |
| Number of Residues | 11 |
| Details | binding site for residue ATP D 602 |
| Chain | Residue |
| C | THR189 |
| D | LYS16 |
| D | GLY17 |
| D | ILE18 |
| D | LYS38 |
| D | ASP70 |
| D | GLU146 |
| D | ARG217 |
| D | VAL253 |
| D | ASP312 |
| D | UTP601 |
| site_id | AC6 |
| Number of Residues | 15 |
| Details | binding site for Di-peptide UTP B 601 and HIS B 55 |
| Chain | Residue |
| A | LYS193 |
| A | THR194 |
| A | LYS195 |
| A | LYS229 |
| B | SER12 |
| B | LYS38 |
| B | TYR42 |
| B | TYR53 |
| B | GLU54 |
| B | GLY56 |
| B | ASP68 |
| B | ASP70 |
| B | GLY149 |
| B | ATP602 |
| D | GLN112 |
| site_id | AC7 |
| Number of Residues | 15 |
| Details | binding site for Di-peptide UTP B 601 and HIS B 55 |
| Chain | Residue |
| A | LYS193 |
| A | THR194 |
| A | LYS195 |
| A | LYS229 |
| B | SER12 |
| B | LYS38 |
| B | TYR42 |
| B | TYR53 |
| B | GLU54 |
| B | GLY56 |
| B | ASP68 |
| B | ASP70 |
| B | GLY149 |
| B | ATP602 |
| D | GLN112 |
| site_id | AC8 |
| Number of Residues | 15 |
| Details | binding site for Di-peptide UTP C 601 and HIS C 55 |
| Chain | Residue |
| D | LYS195 |
| D | LYS229 |
| A | GLN112 |
| C | SER12 |
| C | LYS38 |
| C | TYR42 |
| C | TYR53 |
| C | GLU54 |
| C | GLY56 |
| C | ASP68 |
| C | ASP70 |
| C | GLY149 |
| C | ATP602 |
| D | LYS193 |
| D | THR194 |
| site_id | AC9 |
| Number of Residues | 15 |
| Details | binding site for Di-peptide UTP C 601 and HIS C 55 |
| Chain | Residue |
| A | GLN112 |
| C | SER12 |
| C | LYS38 |
| C | TYR42 |
| C | TYR53 |
| C | GLU54 |
| C | GLY56 |
| C | ASP68 |
| C | ASP70 |
| C | GLY149 |
| C | ATP602 |
| D | LYS193 |
| D | THR194 |
| D | LYS195 |
| D | LYS229 |
| site_id | AD1 |
| Number of Residues | 15 |
| Details | binding site for Di-peptide UTP D 601 and HIS D 55 |
| Chain | Residue |
| B | GLN112 |
| C | LYS193 |
| C | THR194 |
| C | LYS195 |
| C | LYS229 |
| D | SER12 |
| D | LYS38 |
| D | TYR42 |
| D | TYR53 |
| D | GLU54 |
| D | GLY56 |
| D | ASP68 |
| D | ASP70 |
| D | GLY149 |
| D | ATP602 |
| site_id | AD2 |
| Number of Residues | 15 |
| Details | binding site for Di-peptide UTP D 601 and HIS D 55 |
| Chain | Residue |
| B | GLN112 |
| C | LYS193 |
| C | THR194 |
| C | LYS195 |
| C | LYS229 |
| D | SER12 |
| D | LYS38 |
| D | TYR42 |
| D | TYR53 |
| D | GLU54 |
| D | GLY56 |
| D | ASP68 |
| D | ASP70 |
| D | GLY149 |
| D | ATP602 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | ACT_SITE: For GATase activity => ECO:0000255|PROSITE-ProRule:PRU00605 |
| Chain | Residue | Details |
| A | CYS399 | |
| D | CYS399 | |
| D | HIS526 | |
| D | GLU528 | |
| A | HIS526 | |
| A | GLU528 | |
| B | CYS399 | |
| B | HIS526 | |
| B | GLU528 | |
| C | CYS399 | |
| C | HIS526 | |
| C | GLU528 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER568 | |
| B | SER568 | |
| C | SER568 | |
| D | SER568 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER571 | |
| B | SER571 | |
| C | SER571 | |
| D | SER571 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER574 | |
| B | SER574 | |
| C | SER574 | |
| D | SER574 |
