6PJX
Crystal Structure of G Protein-Coupled Receptor Kinase 5 (GRK5) in Complex with Calmodulin (CaM)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0004703 | molecular_function | G protein-coupled receptor kinase activity |
| A | 0005080 | molecular_function | protein kinase C binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005543 | molecular_function | phospholipid binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0006915 | biological_process | apoptotic process |
| A | 0007165 | biological_process | signal transduction |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0007188 | biological_process | adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| A | 0007217 | biological_process | tachykinin receptor signaling pathway |
| A | 0008277 | biological_process | regulation of G protein-coupled receptor signaling pathway |
| A | 0008284 | biological_process | positive regulation of cell population proliferation |
| A | 0008289 | molecular_function | lipid binding |
| A | 0009966 | biological_process | regulation of signal transduction |
| A | 0016055 | biological_process | Wnt signaling pathway |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016607 | cellular_component | nuclear speck |
| A | 0016740 | molecular_function | transferase activity |
| A | 0031965 | cellular_component | nuclear membrane |
| A | 0043066 | biological_process | negative regulation of apoptotic process |
| A | 0045444 | biological_process | fat cell differentiation |
| A | 0047696 | molecular_function | beta-adrenergic receptor kinase activity |
| A | 0051726 | biological_process | regulation of cell cycle |
| B | 0005509 | molecular_function | calcium ion binding |
| B | 0016460 | cellular_component | myosin II complex |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 17 |
| Details | binding site for residue SGV A 601 |
| Chain | Residue |
| A | LEU192 |
| A | LEU318 |
| A | SER328 |
| A | HOH731 |
| A | HOH810 |
| A | HOH952 |
| A | HOH1013 |
| A | HOH1015 |
| A | HOH1049 |
| A | GLY193 |
| A | LYS194 |
| A | ALA213 |
| A | LEU263 |
| A | THR264 |
| A | MET266 |
| A | ASP270 |
| A | GLU315 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue CA B 201 |
| Chain | Residue |
| B | ASP56 |
| B | ASP58 |
| B | ASN60 |
| B | THR62 |
| B | GLU67 |
| B | HOH320 |
| B | HOH325 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue CA B 202 |
| Chain | Residue |
| A | HOH806 |
| B | ASP20 |
| B | ASP22 |
| B | ASP24 |
| B | THR26 |
| B | GLU31 |
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL |
| Chain | Residue | Details |
| B | ASP20-LEU32 | |
| B | ASP56-PHE68 | |
| B | ASP93-LEU105 | |
| B | ASP129-PHE141 |
| site_id | PS00107 |
| Number of Residues | 33 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGKGGFGEVCaCqvratgkmyackrlekk.RIKK |
| Chain | Residue | Details |
| A | LEU192-LYS224 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 118 |
| Details | Domain: {"description":"RGS","evidences":[{"source":"PROSITE-ProRule","id":"PRU00171","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 262 |
| Details | Domain: {"description":"Protein kinase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 65 |
| Details | Domain: {"description":"AGC-kinase C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00618","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 184 |
| Details | Region: {"description":"N-terminal"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 19 |
| Details | Region: {"description":"Interaction with calmodulin"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 7 |
| Details | Motif: {"description":"Nuclear localization signal","evidences":[{"source":"PubMed","id":"15542828","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 9 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by autocatalysis","evidences":[{"source":"PubMed","id":"8144599","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine; by autocatalysis","evidences":[{"source":"PubMed","id":"8144599","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
