6PHA
Protein Tyrosine Phosphatase 1B (1-301), F182A mutant, vanadate bound state
Functional Information from GO Data
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | binding site for residue VO4 A 301 |
| Chain | Residue |
| A | ASP181 |
| A | HOH417 |
| A | CYS215 |
| A | SER216 |
| A | ALA217 |
| A | GLY218 |
| A | ILE219 |
| A | GLY220 |
| A | ARG221 |
| A | GLN262 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 302 |
| Chain | Residue |
| A | ASN42 |
| A | ASN44 |
| A | ARG45 |
| A | LYS239 |
| A | ILE281 |
| A | MET282 |
| A | HOH402 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 303 |
| Chain | Residue |
| A | HIS54 |
| A | GLN127 |
| A | LYS128 |
| A | GLU129 |
| A | GLU130 |
| A | HOH405 |
| A | HOH429 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | binding site for residue GOL A 304 |
| Chain | Residue |
| A | ALA264 |
| A | ASP265 |
| A | ARG268 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 305 |
| Chain | Residue |
| A | GLU159 |
| A | THR168 |
| A | ARG169 |
| A | HOH424 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 306 |
| Chain | Residue |
| A | GLU159 |
| A | GLU161 |
| A | THR168 |
| A | HOH401 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue ACT A 307 |
| Chain | Residue |
| A | GLN127 |
| A | LYS128 |
| A | LYS131 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue ACT A 308 |
| Chain | Residue |
| A | GLU97 |
| A | GLU101 |
| A | THR138 |
| A | ASN162 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue ACT A 309 |
| Chain | Residue |
| A | GLU2 |
| A | MET3 |
| A | GLU4 |
| A | PRO241 |
| A | ALA278 |
| A | HOH409 |
Functional Information from PROSITE/UniProt
| site_id | PS00383 |
| Number of Residues | 11 |
| Details | TYR_PHOSPHATASE_1 Tyrosine specific protein phosphatases active site. VHCsaGigRSG |
| Chain | Residue | Details |
| A | VAL213-GLY223 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 274 |
| Details | Domain: {"description":"Tyrosine-protein phosphatase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00160","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Phosphocysteine intermediate"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"15592455","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by PKB/AKT1, CLK1 and CLK2","evidences":[{"source":"PubMed","id":"10480872","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11579209","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphotyrosine; by EGFR","evidences":[{"source":"PubMed","id":"9355745","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"S-nitrosocysteine; in reversibly inhibited form","evidences":[{"source":"PubMed","id":"22169477","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine; by CLK1 and CLK2","evidences":[{"source":"PubMed","id":"10480872","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Cross-link: {"description":"N,N-(cysteine-1,S-diyl)serine (Cys-Ser); in inhibited form","evidences":[{"source":"PubMed","id":"12802338","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12802339","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 469 |
| Chain | Residue | Details |
| A | ASP181 | proton shuttle (general acid/base) |
| A | CYS215 | covalent catalysis |
| A | ARG221 | activator, electrostatic stabiliser |
| A | SER222 | activator, electrostatic stabiliser |
| A | GLN262 | steric role |
