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6PBX

Single particle cryo-EM structure of the voltage-gated K+ channel Eag1 3-13 deletion mutant bound to calmodulin (conformation 2)

Functional Information from GO Data
ChainGOidnamespacecontents
A0005216molecular_functionmonoatomic ion channel activity
A0005249molecular_functionvoltage-gated potassium channel activity
A0006811biological_processmonoatomic ion transport
A0006813biological_processpotassium ion transport
A0016020cellular_componentmembrane
A0055085biological_processtransmembrane transport
B0000086biological_processG2/M transition of mitotic cell cycle
B0000922cellular_componentspindle pole
B0002027biological_processregulation of heart rate
B0005509molecular_functioncalcium ion binding
B0005513biological_processdetection of calcium ion
B0005515molecular_functionprotein binding
B0005576cellular_componentextracellular region
B0005634cellular_componentnucleus
B0005654cellular_componentnucleoplasm
B0005737cellular_componentcytoplasm
B0005813cellular_componentcentrosome
B0005819cellular_componentspindle
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005876cellular_componentspindle microtubule
B0005886cellular_componentplasma membrane
B0007186biological_processG protein-coupled receptor signaling pathway
B0008076cellular_componentvoltage-gated potassium channel complex
B0010800biological_processpositive regulation of peptidyl-threonine phosphorylation
B0010801biological_processnegative regulation of peptidyl-threonine phosphorylation
B0010856molecular_functionadenylate cyclase activator activity
B0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
B0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
B0016020cellular_componentmembrane
B0016240biological_processautophagosome membrane docking
B0019855molecular_functioncalcium channel inhibitor activity
B0019901molecular_functionprotein kinase binding
B0021762biological_processsubstantia nigra development
B0030017cellular_componentsarcomere
B0031432molecular_functiontitin binding
B0031514cellular_componentmotile cilium
B0031954biological_processpositive regulation of protein autophosphorylation
B0031982cellular_componentvesicle
B0032465biological_processregulation of cytokinesis
B0032991cellular_componentprotein-containing complex
B0034704cellular_componentcalcium channel complex
B0035458biological_processcellular response to interferon-beta
B0043209cellular_componentmyelin sheath
B0043539molecular_functionprotein serine/threonine kinase activator activity
B0044305cellular_componentcalyx of Held
B0044325molecular_functiontransmembrane transporter binding
B0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
B0046872molecular_functionmetal ion binding
B0048306molecular_functioncalcium-dependent protein binding
B0050848biological_processregulation of calcium-mediated signaling
B0051592biological_processresponse to calcium ion
B0055117biological_processregulation of cardiac muscle contraction
B0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
B0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
B0060316biological_processpositive regulation of ryanodine-sensitive calcium-release channel activity
B0071346biological_processcellular response to type II interferon
B0071902biological_processpositive regulation of protein serine/threonine kinase activity
B0072542molecular_functionprotein phosphatase activator activity
B0097225cellular_componentsperm midpiece
B0097720biological_processcalcineurin-mediated signaling
B0098901biological_processregulation of cardiac muscle cell action potential
B0099523cellular_componentpresynaptic cytosol
B0140056biological_processorganelle localization by membrane tethering
B0140238biological_processpresynaptic endocytosis
B1901842biological_processnegative regulation of high voltage-gated calcium channel activity
B1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
B1902494cellular_componentcatalytic complex
B1905913biological_processnegative regulation of calcium ion export across plasma membrane
B1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
C0005216molecular_functionmonoatomic ion channel activity
C0005249molecular_functionvoltage-gated potassium channel activity
C0006811biological_processmonoatomic ion transport
C0006813biological_processpotassium ion transport
C0016020cellular_componentmembrane
C0055085biological_processtransmembrane transport
D0000086biological_processG2/M transition of mitotic cell cycle
D0000922cellular_componentspindle pole
D0002027biological_processregulation of heart rate
D0005509molecular_functioncalcium ion binding
D0005513biological_processdetection of calcium ion
D0005515molecular_functionprotein binding
D0005576cellular_componentextracellular region
D0005634cellular_componentnucleus
D0005654cellular_componentnucleoplasm
D0005737cellular_componentcytoplasm
D0005813cellular_componentcentrosome
D0005819cellular_componentspindle
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005876cellular_componentspindle microtubule
D0005886cellular_componentplasma membrane
D0007186biological_processG protein-coupled receptor signaling pathway
D0008076cellular_componentvoltage-gated potassium channel complex
D0010800biological_processpositive regulation of peptidyl-threonine phosphorylation
D0010801biological_processnegative regulation of peptidyl-threonine phosphorylation
D0010856molecular_functionadenylate cyclase activator activity
D0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
D0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
D0016020cellular_componentmembrane
D0016240biological_processautophagosome membrane docking
D0019855molecular_functioncalcium channel inhibitor activity
D0019901molecular_functionprotein kinase binding
D0021762biological_processsubstantia nigra development
D0030017cellular_componentsarcomere
D0031432molecular_functiontitin binding
D0031514cellular_componentmotile cilium
D0031954biological_processpositive regulation of protein autophosphorylation
D0031982cellular_componentvesicle
D0032465biological_processregulation of cytokinesis
D0032991cellular_componentprotein-containing complex
D0034704cellular_componentcalcium channel complex
D0035458biological_processcellular response to interferon-beta
D0043209cellular_componentmyelin sheath
D0043539molecular_functionprotein serine/threonine kinase activator activity
D0044305cellular_componentcalyx of Held
D0044325molecular_functiontransmembrane transporter binding
D0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
D0046872molecular_functionmetal ion binding
D0048306molecular_functioncalcium-dependent protein binding
D0050848biological_processregulation of calcium-mediated signaling
D0051592biological_processresponse to calcium ion
D0055117biological_processregulation of cardiac muscle contraction
D0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
D0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
D0060316biological_processpositive regulation of ryanodine-sensitive calcium-release channel activity
D0071346biological_processcellular response to type II interferon
D0071902biological_processpositive regulation of protein serine/threonine kinase activity
D0072542molecular_functionprotein phosphatase activator activity
D0097225cellular_componentsperm midpiece
D0097720biological_processcalcineurin-mediated signaling
D0098901biological_processregulation of cardiac muscle cell action potential
D0099523cellular_componentpresynaptic cytosol
D0140056biological_processorganelle localization by membrane tethering
D0140238biological_processpresynaptic endocytosis
D1901842biological_processnegative regulation of high voltage-gated calcium channel activity
D1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
D1902494cellular_componentcatalytic complex
D1905913biological_processnegative regulation of calcium ion export across plasma membrane
D1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
E0005216molecular_functionmonoatomic ion channel activity
E0005249molecular_functionvoltage-gated potassium channel activity
E0006811biological_processmonoatomic ion transport
E0006813biological_processpotassium ion transport
E0016020cellular_componentmembrane
E0055085biological_processtransmembrane transport
F0000086biological_processG2/M transition of mitotic cell cycle
F0000922cellular_componentspindle pole
F0002027biological_processregulation of heart rate
F0005509molecular_functioncalcium ion binding
F0005513biological_processdetection of calcium ion
F0005515molecular_functionprotein binding
F0005576cellular_componentextracellular region
F0005634cellular_componentnucleus
F0005654cellular_componentnucleoplasm
F0005737cellular_componentcytoplasm
F0005813cellular_componentcentrosome
F0005819cellular_componentspindle
F0005829cellular_componentcytosol
F0005856cellular_componentcytoskeleton
F0005876cellular_componentspindle microtubule
F0005886cellular_componentplasma membrane
F0007186biological_processG protein-coupled receptor signaling pathway
F0008076cellular_componentvoltage-gated potassium channel complex
F0010800biological_processpositive regulation of peptidyl-threonine phosphorylation
F0010801biological_processnegative regulation of peptidyl-threonine phosphorylation
F0010856molecular_functionadenylate cyclase activator activity
F0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
F0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
F0016020cellular_componentmembrane
F0016240biological_processautophagosome membrane docking
F0019855molecular_functioncalcium channel inhibitor activity
F0019901molecular_functionprotein kinase binding
F0021762biological_processsubstantia nigra development
F0030017cellular_componentsarcomere
F0031432molecular_functiontitin binding
F0031514cellular_componentmotile cilium
F0031954biological_processpositive regulation of protein autophosphorylation
F0031982cellular_componentvesicle
F0032465biological_processregulation of cytokinesis
F0032991cellular_componentprotein-containing complex
F0034704cellular_componentcalcium channel complex
F0035458biological_processcellular response to interferon-beta
F0043209cellular_componentmyelin sheath
F0043539molecular_functionprotein serine/threonine kinase activator activity
F0044305cellular_componentcalyx of Held
F0044325molecular_functiontransmembrane transporter binding
F0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
F0046872molecular_functionmetal ion binding
F0048306molecular_functioncalcium-dependent protein binding
F0050848biological_processregulation of calcium-mediated signaling
F0051592biological_processresponse to calcium ion
F0055117biological_processregulation of cardiac muscle contraction
F0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
F0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
F0060316biological_processpositive regulation of ryanodine-sensitive calcium-release channel activity
F0071346biological_processcellular response to type II interferon
F0071902biological_processpositive regulation of protein serine/threonine kinase activity
F0072542molecular_functionprotein phosphatase activator activity
F0097225cellular_componentsperm midpiece
F0097720biological_processcalcineurin-mediated signaling
F0098901biological_processregulation of cardiac muscle cell action potential
F0099523cellular_componentpresynaptic cytosol
F0140056biological_processorganelle localization by membrane tethering
F0140238biological_processpresynaptic endocytosis
F1901842biological_processnegative regulation of high voltage-gated calcium channel activity
F1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
F1902494cellular_componentcatalytic complex
F1905913biological_processnegative regulation of calcium ion export across plasma membrane
F1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
G0005216molecular_functionmonoatomic ion channel activity
G0005249molecular_functionvoltage-gated potassium channel activity
G0006811biological_processmonoatomic ion transport
G0006813biological_processpotassium ion transport
G0016020cellular_componentmembrane
G0055085biological_processtransmembrane transport
H0000086biological_processG2/M transition of mitotic cell cycle
H0000922cellular_componentspindle pole
H0002027biological_processregulation of heart rate
H0005509molecular_functioncalcium ion binding
H0005513biological_processdetection of calcium ion
H0005515molecular_functionprotein binding
H0005576cellular_componentextracellular region
H0005634cellular_componentnucleus
H0005654cellular_componentnucleoplasm
H0005737cellular_componentcytoplasm
H0005813cellular_componentcentrosome
H0005819cellular_componentspindle
H0005829cellular_componentcytosol
H0005856cellular_componentcytoskeleton
H0005876cellular_componentspindle microtubule
H0005886cellular_componentplasma membrane
H0007186biological_processG protein-coupled receptor signaling pathway
H0008076cellular_componentvoltage-gated potassium channel complex
H0010800biological_processpositive regulation of peptidyl-threonine phosphorylation
H0010801biological_processnegative regulation of peptidyl-threonine phosphorylation
H0010856molecular_functionadenylate cyclase activator activity
H0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
H0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
H0016020cellular_componentmembrane
H0016240biological_processautophagosome membrane docking
H0019855molecular_functioncalcium channel inhibitor activity
H0019901molecular_functionprotein kinase binding
H0021762biological_processsubstantia nigra development
H0030017cellular_componentsarcomere
H0031432molecular_functiontitin binding
H0031514cellular_componentmotile cilium
H0031954biological_processpositive regulation of protein autophosphorylation
H0031982cellular_componentvesicle
H0032465biological_processregulation of cytokinesis
H0032991cellular_componentprotein-containing complex
H0034704cellular_componentcalcium channel complex
H0035458biological_processcellular response to interferon-beta
H0043209cellular_componentmyelin sheath
H0043539molecular_functionprotein serine/threonine kinase activator activity
H0044305cellular_componentcalyx of Held
H0044325molecular_functiontransmembrane transporter binding
H0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
H0046872molecular_functionmetal ion binding
H0048306molecular_functioncalcium-dependent protein binding
H0050848biological_processregulation of calcium-mediated signaling
H0051592biological_processresponse to calcium ion
H0055117biological_processregulation of cardiac muscle contraction
H0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
H0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
H0060316biological_processpositive regulation of ryanodine-sensitive calcium-release channel activity
H0071346biological_processcellular response to type II interferon
H0071902biological_processpositive regulation of protein serine/threonine kinase activity
H0072542molecular_functionprotein phosphatase activator activity
H0097225cellular_componentsperm midpiece
H0097720biological_processcalcineurin-mediated signaling
H0098901biological_processregulation of cardiac muscle cell action potential
H0099523cellular_componentpresynaptic cytosol
H0140056biological_processorganelle localization by membrane tethering
H0140238biological_processpresynaptic endocytosis
H1901842biological_processnegative regulation of high voltage-gated calcium channel activity
H1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
H1902494cellular_componentcatalytic complex
H1905913biological_processnegative regulation of calcium ion export across plasma membrane
H1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
Functional Information from PROSITE/UniProt
site_idPS00018
Number of Residues13
DetailsEF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL
ChainResidueDetails
BASP20-LEU32
BASP56-PHE68
BASP93-LEU105
BASP129-PHE141

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues40
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:25441029, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:4UMO, ECO:0007744|PDB:4V0C, ECO:0007744|PDB:5J03
ChainResidueDetails
BASP20
BGLU67
DASP20
DASP22
DASP24
DTHR26
DGLU31
DASP56
DASP58
DASN60
DTHR62
BASP22
DGLU67
FASP20
FASP22
FASP24
FTHR26
FGLU31
FASP56
FASP58
FASN60
FTHR62
BASP24
FGLU67
HASP20
HASP22
HASP24
HTHR26
HGLU31
HASP56
HASP58
HASN60
HTHR62
BTHR26
HGLU67
BGLU31
BASP56
BASP58
BASN60
BTHR62

site_idSWS_FT_FI2
Number of Residues20
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0000269|PubMed:29724949, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03, ECO:0007744|PDB:6CNN, ECO:0007744|PDB:6CNO
ChainResidueDetails
BASP93
DGLU104
FASP93
FASP95
FASN97
FTYR99
FGLU104
HASP93
HASP95
HASN97
HTYR99
BASP95
HGLU104
BASN97
BTYR99
BGLU104
DASP93
DASP95
DASN97
DTYR99

site_idSWS_FT_FI3
Number of Residues20
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03
ChainResidueDetails
BASP129
DGLU140
FASP129
FASP131
FASP133
FGLN135
FGLU140
HASP129
HASP131
HASP133
HGLN135
BASP131
HGLU140
BASP133
BGLN135
BGLU140
DASP129
DASP131
DASP133
DGLN135

site_idSWS_FT_FI4
Number of Residues4
DetailsMOD_RES: N-acetylalanine => ECO:0000269|PubMed:7093203, ECO:0000269|Ref.7, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712
ChainResidueDetails
BALA1
GHIS270-ASN290
GASP342-ALA350
DALA1
FALA1
HALA1
CASP342-ALA350
EHIS270-ASN290
EASP342-ALA350

site_idSWS_FT_FI5
Number of Residues4
DetailsMOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861
ChainResidueDetails
BLYS21
DLYS21
FLYS21
HLYS21

site_idSWS_FT_FI6
Number of Residues4
DetailsMOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29
ChainResidueDetails
BTHR44
DTHR44
FTHR44
HTHR44

site_idSWS_FT_FI7
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163
ChainResidueDetails
BSER81
DSER81
FSER81
HSER81

site_idSWS_FT_FI8
Number of Residues4
DetailsMOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861
ChainResidueDetails
BLYS94
DLYS94
FLYS94
HLYS94

site_idSWS_FT_FI9
Number of Residues4
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332
ChainResidueDetails
BTYR99
DTYR99
FTYR99
HTYR99

site_idSWS_FT_FI10
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569
ChainResidueDetails
BSER101
GSER833
GSER837
GSER840
DSER101
FSER101
HSER101
CSER837
CSER840
ESER833
ESER837
ESER840

site_idSWS_FT_FI11
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569
ChainResidueDetails
BTHR110
DTHR110
FTHR110
HTHR110

site_idSWS_FT_FI12
Number of Residues4
DetailsMOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315
ChainResidueDetails
BLYS115
DLYS115
FLYS115
HLYS115

site_idSWS_FT_FI13
Number of Residues4
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332
ChainResidueDetails
BTYR138
DTYR138
FTYR138
HTYR138

site_idSWS_FT_FI14
Number of Residues8
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157
ChainResidueDetails
BLYS21
DLYS21
FLYS21
HLYS21

227344

PDB entries from 2024-11-13

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