6O83

S. pombe ubiquitin E1~ubiquitin-AMP tetrahedral intermediate mimic

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0004839	molecular_function	ubiquitin activating enzyme activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0006974	biological_process	DNA damage response
A	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
A	0016567	biological_process	protein ubiquitination
A	0016874	molecular_function	ligase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0036211	biological_process	protein modification process
A	0046872	molecular_function	metal ion binding
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0004839	molecular_function	ubiquitin activating enzyme activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0006974	biological_process	DNA damage response
C	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
C	0016567	biological_process	protein ubiquitination
C	0016874	molecular_function	ligase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0036211	biological_process	protein modification process
C	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue CA A 1101

Chain	Residue
A	HIS305
A	GLU320
A	ASP715
A	HIS717

---

site_id	AC2
Number of Residues	4
Details	binding site for residue TRS A 1102

Chain	Residue
A	GLU553
A	ARG919
A	ASN921
A	CYS1009

---

site_id	AC3
Number of Residues	2
Details	binding site for residue MPD A 1103

Chain	Residue
A	TYR378
A	THR175

---

site_id	AC4
Number of Residues	4
Details	binding site for residue MPD A 1104

Chain	Residue
A	GLN136
A	SER161
A	PHE295
A	TYR385

---

site_id	AC5
Number of Residues	3
Details	binding site for residue MPD A 1105

Chain	Residue
A	GLU658
A	GLU665
A	MET668

---

site_id	AC6
Number of Residues	4
Details	binding site for residue MPD A 1106

Chain	Residue
A	ARG547
A	VAL990
A	GLU992
A	PHE1007

---

site_id	AC7
Number of Residues	3
Details	binding site for residue MPD A 1107

Chain	Residue
A	ILE716
A	HIS717
A	GLU748

---

site_id	AC8
Number of Residues	13
Details	binding site for residue VMX A 1108

Chain	Residue
A	ALA437
A	ILE438
A	ASP463
A	MET464
A	ASP465
A	LYS487
A	VAL513
A	ALA535
A	ASP537
A	ASN538
A	THR561
A	CYS593
B	GLY75

---

site_id	AC9
Number of Residues	4
Details	binding site for residue CA C 1101

Chain	Residue
C	HIS305
C	GLU320
C	ASP715
C	HIS717

---

site_id	AD1
Number of Residues	6
Details	binding site for residue TRS C 1102

Chain	Residue
C	GLU553
C	ARG919
C	ASN921
C	HIS988
C	CYS1009
C	LYS1011

---

site_id	AD2
Number of Residues	3
Details	binding site for residue MPD C 1103

Chain	Residue
C	GLU658
C	GLU665
C	LEU672

---

site_id	AD3
Number of Residues	5
Details	binding site for residue MPD C 1104

Chain	Residue
C	ASP68
C	PRO69
C	GLN70
C	GLU130
C	THR131

---

site_id	AD4
Number of Residues	2
Details	binding site for residue MPD C 1105

Chain	Residue
C	GLU577
C	SER582

---

site_id	AD5
Number of Residues	3
Details	binding site for residue MPD C 1106

Chain	Residue
C	ILE716
C	PHE722
C	GLU748

---

site_id	AD6
Number of Residues	5
Details	binding site for residue MPD C 1107

Chain	Residue
C	LEU133
C	GLN136
C	SER161
C	PHE295
C	TYR385

---

site_id	AD7
Number of Residues	3
Details	binding site for residue MPD C 1108

Chain	Residue
C	VAL990
C	GLU992
C	PHE1007

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KskIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
B	LYS27-ASP52

---

site_id	PS00865
Number of Residues	9
Details	UBIQUITIN_ACTIVAT_2 Ubiquitin-activating enzyme active site. PICTLKnFP

Chain	Residue	Details
A	PRO591-PRO599

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU10132","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23416107","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4II2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4II3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P22515","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23416107","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4II2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P22515","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P22515","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---