6O4K

Structure of ALDH7A1 mutant E399Q complexed with NAD

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000139	cellular_component	Golgi membrane
A	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
A	0004043	molecular_function	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006081	biological_process	aldehyde metabolic process
A	0006554	biological_process	obsolete lysine catabolic process
A	0007605	biological_process	sensory perception of sound
A	0008802	molecular_function	betaine-aldehyde dehydrogenase (NAD+) activity
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0019285	biological_process	glycine betaine biosynthetic process from choline
A	0019477	biological_process	L-lysine catabolic process
A	0042426	biological_process	choline catabolic process
A	0042802	molecular_function	identical protein binding
A	0070062	cellular_component	extracellular exosome
B	0000139	cellular_component	Golgi membrane
B	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
B	0004043	molecular_function	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006081	biological_process	aldehyde metabolic process
B	0006554	biological_process	obsolete lysine catabolic process
B	0007605	biological_process	sensory perception of sound
B	0008802	molecular_function	betaine-aldehyde dehydrogenase (NAD+) activity
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0019285	biological_process	glycine betaine biosynthetic process from choline
B	0019477	biological_process	L-lysine catabolic process
B	0042426	biological_process	choline catabolic process
B	0042802	molecular_function	identical protein binding
B	0070062	cellular_component	extracellular exosome
C	0000139	cellular_component	Golgi membrane
C	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
C	0004043	molecular_function	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006081	biological_process	aldehyde metabolic process
C	0006554	biological_process	obsolete lysine catabolic process
C	0007605	biological_process	sensory perception of sound
C	0008802	molecular_function	betaine-aldehyde dehydrogenase (NAD+) activity
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0019285	biological_process	glycine betaine biosynthetic process from choline
C	0019477	biological_process	L-lysine catabolic process
C	0042426	biological_process	choline catabolic process
C	0042802	molecular_function	identical protein binding
C	0070062	cellular_component	extracellular exosome
D	0000139	cellular_component	Golgi membrane
D	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
D	0004043	molecular_function	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006081	biological_process	aldehyde metabolic process
D	0006554	biological_process	obsolete lysine catabolic process
D	0007605	biological_process	sensory perception of sound
D	0008802	molecular_function	betaine-aldehyde dehydrogenase (NAD+) activity
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0019285	biological_process	glycine betaine biosynthetic process from choline
D	0019477	biological_process	L-lysine catabolic process
D	0042426	biological_process	choline catabolic process
D	0042802	molecular_function	identical protein binding
D	0070062	cellular_component	extracellular exosome
E	0000139	cellular_component	Golgi membrane
E	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
E	0004043	molecular_function	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005739	cellular_component	mitochondrion
E	0005759	cellular_component	mitochondrial matrix
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006081	biological_process	aldehyde metabolic process
E	0006554	biological_process	obsolete lysine catabolic process
E	0007605	biological_process	sensory perception of sound
E	0008802	molecular_function	betaine-aldehyde dehydrogenase (NAD+) activity
E	0016491	molecular_function	oxidoreductase activity
E	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
E	0019285	biological_process	glycine betaine biosynthetic process from choline
E	0019477	biological_process	L-lysine catabolic process
E	0042426	biological_process	choline catabolic process
E	0042802	molecular_function	identical protein binding
E	0070062	cellular_component	extracellular exosome
F	0000139	cellular_component	Golgi membrane
F	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
F	0004043	molecular_function	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006081	biological_process	aldehyde metabolic process
F	0006554	biological_process	obsolete lysine catabolic process
F	0007605	biological_process	sensory perception of sound
F	0008802	molecular_function	betaine-aldehyde dehydrogenase (NAD+) activity
F	0016491	molecular_function	oxidoreductase activity
F	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F	0019285	biological_process	glycine betaine biosynthetic process from choline
F	0019477	biological_process	L-lysine catabolic process
F	0042426	biological_process	choline catabolic process
F	0042802	molecular_function	identical protein binding
F	0070062	cellular_component	extracellular exosome
G	0000139	cellular_component	Golgi membrane
G	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
G	0004043	molecular_function	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005739	cellular_component	mitochondrion
G	0005759	cellular_component	mitochondrial matrix
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0006081	biological_process	aldehyde metabolic process
G	0006554	biological_process	obsolete lysine catabolic process
G	0007605	biological_process	sensory perception of sound
G	0008802	molecular_function	betaine-aldehyde dehydrogenase (NAD+) activity
G	0016491	molecular_function	oxidoreductase activity
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0019285	biological_process	glycine betaine biosynthetic process from choline
G	0019477	biological_process	L-lysine catabolic process
G	0042426	biological_process	choline catabolic process
G	0042802	molecular_function	identical protein binding
G	0070062	cellular_component	extracellular exosome
H	0000139	cellular_component	Golgi membrane
H	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
H	0004043	molecular_function	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005739	cellular_component	mitochondrion
H	0005759	cellular_component	mitochondrial matrix
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0006081	biological_process	aldehyde metabolic process
H	0006554	biological_process	obsolete lysine catabolic process
H	0007605	biological_process	sensory perception of sound
H	0008802	molecular_function	betaine-aldehyde dehydrogenase (NAD+) activity
H	0016491	molecular_function	oxidoreductase activity
H	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
H	0019285	biological_process	glycine betaine biosynthetic process from choline
H	0019477	biological_process	L-lysine catabolic process
H	0042426	biological_process	choline catabolic process
H	0042802	molecular_function	identical protein binding
H	0070062	cellular_component	extracellular exosome

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue EDO A 601

Chain	Residue
A	ASN28
A	ASP59
A	HOH720

---

site_id	AC2
Number of Residues	15
Details	binding site for residue NAD A 602

Chain	Residue
A	GLY230
A	THR231
A	PHE244
A	THR245
A	GLY246
A	SER247
A	VAL250
A	HOH820
A	HOH873
A	ILE163
A	THR164
A	LYS190
A	ALA192
A	PRO193
A	ALA227

---

site_id	AC3
Number of Residues	2
Details	binding site for residue CL A 603

Chain	Residue
A	ARG321
B	GLN511

---

site_id	AC4
Number of Residues	4
Details	binding site for residue EDO B 601

Chain	Residue
B	GLU36
B	GLN54
E	SER394
E	HOH772

---

site_id	AC5
Number of Residues	20
Details	binding site for residue NAD B 602

Chain	Residue
B	ILE163
B	THR164
B	ALA165
B	PHE166
B	LYS190
B	GLY191
B	ALA192
B	PRO193
B	GLY226
B	ALA227
B	GLY230
B	THR231
B	PHE244
B	THR245
B	GLY246
B	SER247
B	VAL250
B	PHE401
B	HOH702
B	HOH718

---

site_id	AC6
Number of Residues	3
Details	binding site for residue CL B 603

Chain	Residue
A	GLN511
B	VAL287
B	ARG321

---

site_id	AC7
Number of Residues	17
Details	binding site for residue NAD C 601

Chain	Residue
C	ILE163
C	THR164
C	ALA165
C	PHE166
C	LYS190
C	GLY191
C	ALA192
C	PRO193
C	ALA227
C	GLY230
C	THR231
C	PHE244
C	THR245
C	GLY246
C	SER247
C	VAL250
C	HOH729

---

site_id	AC8
Number of Residues	4
Details	binding site for residue CL C 602

Chain	Residue
C	VAL287
C	ARG321
D	PHE510
D	GLN511

---

site_id	AC9
Number of Residues	15
Details	binding site for residue NAD D 601

Chain	Residue
D	ILE163
D	THR164
D	ALA165
D	LYS190
D	GLY191
D	ALA192
D	PRO193
D	ALA227
D	GLY230
D	THR231
D	PHE244
D	SER247
D	VAL250
D	VAL254
D	HOH718

---

site_id	AD1
Number of Residues	4
Details	binding site for residue CL D 602

Chain	Residue
C	PHE510
C	GLN511
D	VAL287
D	ARG321

---

site_id	AD2
Number of Residues	12
Details	binding site for residue NAD E 601

Chain	Residue
E	ILE163
E	THR164
E	LYS190
E	GLY191
E	ALA192
E	PRO193
E	ALA227
E	GLY230
E	THR231
E	PHE244
E	SER247
E	VAL250

---

site_id	AD3
Number of Residues	2
Details	binding site for residue CL E 602

Chain	Residue
E	ARG321
F	GLN511

---

site_id	AD4
Number of Residues	3
Details	binding site for residue CL E 603

Chain	Residue
E	GLN511
F	VAL287
F	ARG321

---

site_id	AD5
Number of Residues	3
Details	binding site for residue EDO F 601

Chain	Residue
E	GLN259
F	SER265
F	HOH710

---

site_id	AD6
Number of Residues	6
Details	binding site for residue EDO F 602

Chain	Residue
A	SER394
A	HOH742
F	GLU36
F	GLN54
F	PRO193
F	GLY226

---

site_id	AD7
Number of Residues	17
Details	binding site for residue NAD F 603

Chain	Residue
F	ILE163
F	THR164
F	PHE166
F	LYS190
F	GLY191
F	ALA192
F	PRO193
F	ALA227
F	GLY230
F	THR231
F	PHE244
F	THR245
F	GLY246
F	SER247
F	VAL250
F	VAL254
F	HOH806

---

site_id	AD8
Number of Residues	15
Details	binding site for residue NAD G 601

Chain	Residue
G	ILE163
G	THR164
G	PHE166
G	LYS190
G	ALA227
G	GLY230
G	THR231
G	PHE244
G	THR245
G	GLY246
G	SER247
G	VAL250
G	VAL254
G	HOH701
G	HOH790

---

site_id	AD9
Number of Residues	3
Details	binding site for residue CL G 602

Chain	Residue
G	VAL287
G	ARG321
H	GLN511

---

site_id	AE1
Number of Residues	14
Details	binding site for residue NAD H 601

Chain	Residue
H	ILE163
H	THR164
H	PHE166
H	LYS190
H	GLY191
H	PRO193
H	ALA227
H	GLY230
H	THR231
H	PHE244
H	SER247
H	VAL250
H	VAL254
H	HOH790

---

site_id	AE2
Number of Residues	1
Details	binding site for residue CL H 602

Chain	Residue
H	ARG321

---

Functional Information from PROSITE/UniProt

site_id	PS00687
Number of Residues	8
Details	ALDEHYDE_DEHYDR_GLU Aldehyde dehydrogenases glutamic acid active site. LELGGNNA

Chain	Residue	Details
A	LEU267-ALA274

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10007","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"25554827","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"40233740","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"25554827","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26260980","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	56
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20207735","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2J6L","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PDB","id":"4ZUL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20207735","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38604394","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2J6L","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6V0Z","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"UniProtKB","id":"P20000","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"Q9DBF1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine; by AMPK","evidences":[{"source":"PubMed","id":"40233740","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	16
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q9DBF1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q9DBF1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---