6O20

Cryo-EM structure of TRPV5 with calmodulin bound

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0005261	molecular_function	monoatomic cation channel activity
A	0005262	molecular_function	calcium channel activity
A	0005516	molecular_function	calmodulin binding
A	0005886	cellular_component	plasma membrane
A	0006811	biological_process	monoatomic ion transport
A	0006816	biological_process	calcium ion transport
A	0016020	cellular_component	membrane
A	0016324	cellular_component	apical plasma membrane
A	0035809	biological_process	regulation of urine volume
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0051289	biological_process	protein homotetramerization
A	0055074	biological_process	calcium ion homeostasis
A	0055085	biological_process	transmembrane transport
A	0070588	biological_process	calcium ion transmembrane transport
A	0098703	biological_process	calcium ion import across plasma membrane
B	0005216	molecular_function	monoatomic ion channel activity
B	0005261	molecular_function	monoatomic cation channel activity
B	0005262	molecular_function	calcium channel activity
B	0005516	molecular_function	calmodulin binding
B	0005886	cellular_component	plasma membrane
B	0006811	biological_process	monoatomic ion transport
B	0006816	biological_process	calcium ion transport
B	0016020	cellular_component	membrane
B	0016324	cellular_component	apical plasma membrane
B	0035809	biological_process	regulation of urine volume
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0051289	biological_process	protein homotetramerization
B	0055074	biological_process	calcium ion homeostasis
B	0055085	biological_process	transmembrane transport
B	0070588	biological_process	calcium ion transmembrane transport
B	0098703	biological_process	calcium ion import across plasma membrane
C	0005216	molecular_function	monoatomic ion channel activity
C	0005261	molecular_function	monoatomic cation channel activity
C	0005262	molecular_function	calcium channel activity
C	0005516	molecular_function	calmodulin binding
C	0005886	cellular_component	plasma membrane
C	0006811	biological_process	monoatomic ion transport
C	0006816	biological_process	calcium ion transport
C	0016020	cellular_component	membrane
C	0016324	cellular_component	apical plasma membrane
C	0035809	biological_process	regulation of urine volume
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0051289	biological_process	protein homotetramerization
C	0055074	biological_process	calcium ion homeostasis
C	0055085	biological_process	transmembrane transport
C	0070588	biological_process	calcium ion transmembrane transport
C	0098703	biological_process	calcium ion import across plasma membrane
D	0005216	molecular_function	monoatomic ion channel activity
D	0005261	molecular_function	monoatomic cation channel activity
D	0005262	molecular_function	calcium channel activity
D	0005516	molecular_function	calmodulin binding
D	0005886	cellular_component	plasma membrane
D	0006811	biological_process	monoatomic ion transport
D	0006816	biological_process	calcium ion transport
D	0016020	cellular_component	membrane
D	0016324	cellular_component	apical plasma membrane
D	0035809	biological_process	regulation of urine volume
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	0051289	biological_process	protein homotetramerization
D	0055074	biological_process	calcium ion homeostasis
D	0055085	biological_process	transmembrane transport
D	0070588	biological_process	calcium ion transmembrane transport
D	0098703	biological_process	calcium ion import across plasma membrane
E	0005216	molecular_function	monoatomic ion channel activity
E	0005261	molecular_function	monoatomic cation channel activity
E	0005262	molecular_function	calcium channel activity
E	0005516	molecular_function	calmodulin binding
E	0005886	cellular_component	plasma membrane
E	0006811	biological_process	monoatomic ion transport
E	0006816	biological_process	calcium ion transport
E	0016020	cellular_component	membrane
E	0016324	cellular_component	apical plasma membrane
E	0035809	biological_process	regulation of urine volume
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
E	0051289	biological_process	protein homotetramerization
E	0055074	biological_process	calcium ion homeostasis
E	0055085	biological_process	transmembrane transport
E	0070588	biological_process	calcium ion transmembrane transport
E	0098703	biological_process	calcium ion import across plasma membrane
F	0000922	cellular_component	spindle pole
F	0005509	molecular_function	calcium ion binding
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005819	cellular_component	spindle
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
F	0019904	molecular_function	protein domain specific binding
F	0032991	cellular_component	protein-containing complex
F	0046872	molecular_function	metal ion binding
F	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
F	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue CA F 201

Chain	Residue
F	ASP129
F	ASP131
F	ASP133
F	GLN135
F	ASN137

---

site_id	AC2
Number of Residues	6
Details	binding site for residue CA F 202

Chain	Residue
F	ILE100
F	GLU104
F	ASP93
F	ASP95
F	ASN97
F	TYR99

---

site_id	AC3
Number of Residues	4
Details	binding site for residue CA F 203

Chain	Residue
F	ASP20
F	GLY25
F	THR26
F	GLU31

---

site_id	AC4
Number of Residues	6
Details	binding site for residue CA F 204

Chain	Residue
F	ASP56
F	ASP58
F	ASN60
F	THR62
F	ASP64
F	GLU67

---

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
F	ASP20-LEU32
F	ASP56-PHE68
F	ASP93-LEU105
F	ASP129-PHE141

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448

Chain	Residue	Details
F	ASP20
F	GLU67
F	ASP93
F	ASP95
F	ASN97
F	TYR99
F	GLU104
F	ASP129
F	ASP131
F	ASP133
F	GLN135
F	ASP22
F	GLU140
F	ASP24
F	THR26
F	GLU31
F	ASP56
F	ASP58
F	ASN60
F	THR62

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:7356670

Chain	Residue	Details
F	ALA1
E	GLY449-ALA469
E	PHE493-GLN513
E	ILE557-MET577
B	TYR328-ILE348
B	LEU386-PHE408
B	ILE420-MET442
B	GLY449-ALA469
B	PHE493-GLN513
B	ILE557-MET577
C	TYR328-ILE348
A	LEU386-PHE408
C	LEU386-PHE408
C	ILE420-MET442
C	GLY449-ALA469
C	PHE493-GLN513
C	ILE557-MET577
D	TYR328-ILE348
D	LEU386-PHE408
D	ILE420-MET442
D	GLY449-ALA469
D	PHE493-GLN513
A	ILE420-MET442
D	ILE557-MET577
A	GLY449-ALA469
A	PHE493-GLN513
A	ILE557-MET577
E	TYR328-ILE348
E	LEU386-PHE408
E	ILE420-MET442

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P0DP23

Chain	Residue	Details
F	LYS21
D	ARG443-ASN448
A	ARG443-ASN448
E	TYR349-ARG385
E	ARG443-ASN448
B	TYR349-ARG385
B	ARG443-ASN448
C	TYR349-ARG385
C	ARG443-ASN448
D	TYR349-ARG385

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29

Chain	Residue	Details
F	THR44
E	SER524-PRO544
B	SER524-PRO544
C	SER524-PRO544
D	SER524-PRO544

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P0DP23

Chain	Residue	Details
F	SER81
F	SER101
B	ASP542
C	ASP542
D	ASP542

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P0DP23

Chain	Residue	Details
F	LYS94
E	THR685
B	THR685
C	THR685
D	THR685

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P0DP23

Chain	Residue	Details
F	TYR99
F	TYR138
B	SER689
C	SER689
D	SER689

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P0DP23

Chain	Residue	Details
F	THR110
E	ASN358
B	ASN358
C	ASN358
D	ASN358

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: N6-methyllysine; alternate => ECO:0000250|UniProtKB:P0DP23

Chain	Residue	Details
F	LYS115

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269|PubMed:9716384

Chain	Residue	Details
F	LYS21

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