6NPL
Cryo-EM structure of NKCC1
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0015377 | molecular_function | chloride:monoatomic cation symporter activity |
| A | 0016020 | cellular_component | membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0055085 | biological_process | transmembrane transport |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0015377 | molecular_function | chloride:monoatomic cation symporter activity |
| B | 0016020 | cellular_component | membrane |
| B | 0022857 | molecular_function | transmembrane transporter activity |
| B | 0055085 | biological_process | transmembrane transport |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue K A 1201 |
| Chain | Residue |
| A | ASN220 |
| A | ILE221 |
| A | TYR305 |
| A | PRO417 |
| A | THR420 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue CL A 1202 |
| Chain | Residue |
| A | GLY223 |
| A | VAL224 |
| A | MET225 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue CL A 1203 |
| Chain | Residue |
| A | GLY421 |
| A | ILE422 |
| A | LEU423 |
| A | PHE607 |
| A | TYR611 |
| A | PHE294 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue POV A 1204 |
| Chain | Residue |
| A | ALA609 |
| A | LEU623 |
| A | ASN625 |
| A | ASN664 |
| A | POV1206 |
| B | SER669 |
| B | TYR673 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue POV A 1205 |
| Chain | Residue |
| A | ILE654 |
| A | ASN656 |
| B | TRP657 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for residue POV A 1206 |
| Chain | Residue |
| A | TRP639 |
| A | POV1204 |
| B | SER669 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue K B 1201 |
| Chain | Residue |
| B | ASN220 |
| B | ILE221 |
| B | TYR305 |
| B | PRO417 |
| B | THR420 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue CL B 1202 |
| Chain | Residue |
| B | GLY223 |
| B | VAL224 |
| B | MET225 |
| B | TYR305 |
| B | ALA418 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue CL B 1203 |
| Chain | Residue |
| B | PHE294 |
| B | GLY421 |
| B | ILE422 |
| B | LEU423 |
| B | PHE607 |
| B | TYR611 |
| site_id | AD1 |
| Number of Residues | 4 |
| Details | binding site for residue POV B 1204 |
| Chain | Residue |
| A | TRP657 |
| B | ILE654 |
| B | ILE655 |
| B | TRP657 |
| site_id | AD2 |
| Number of Residues | 8 |
| Details | binding site for residue POV B 1205 |
| Chain | Residue |
| A | SER669 |
| A | TYR673 |
| A | TYR676 |
| B | LEU623 |
| B | ASN625 |
| B | TRP639 |
| B | ASN664 |
| B | POV1207 |
| site_id | AD3 |
| Number of Residues | 12 |
| Details | binding site for residue POV B 1206 |
| Chain | Residue |
| A | PHE616 |
| A | CYS649 |
| A | TYR671 |
| A | TYR676 |
| B | HIS620 |
| B | CYS649 |
| B | PHE653 |
| B | ASN664 |
| B | VAL667 |
| B | TYR671 |
| B | SER675 |
| B | TYR676 |
| site_id | AD4 |
| Number of Residues | 4 |
| Details | binding site for residue POV B 1207 |
| Chain | Residue |
| A | SER669 |
| B | MET638 |
| B | TRP639 |
| B | POV1205 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 50 |
| Details | TRANSMEM: Discontinuously helical; Name=1 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | TRP209-VAL234 | |
| B | TRP209-VAL234 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 210 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | GLY235-GLY238 | |
| A | GLU315-GLN327 | |
| A | ILE377-THR407 | |
| A | ALA463-GLY523 | |
| B | GLY235-GLY238 | |
| B | GLU315-GLN327 | |
| B | ILE377-THR407 | |
| B | ALA463-GLY523 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical; Name=2 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | ILE239-SER261 | |
| B | ILE239-SER261 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1072 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | ALA262-GLU285 | |
| A | ILE428-ALA438 | |
| A | GLN552-PRO576 | |
| A | HIS620-TYR636 | |
| B | ALA262-GLU285 | |
| B | ILE428-ALA438 | |
| B | GLN552-PRO576 | |
| B | HIS620-TYR636 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 56 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | PHE286-VAL314 | |
| B | PHE286-VAL314 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 46 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | THR328-GLU351 | |
| B | THR328-GLU351 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | TRP352-PHE376 | |
| B | TRP352-PHE376 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 38 |
| Details | TRANSMEM: Discontinuously helical; Name=6 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | PHE408-ASN427 | |
| B | PHE408-ASN427 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 46 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | ILE439-GLY462 | |
| B | ILE439-GLY462 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 54 |
| Details | TRANSMEM: Helical; Name=8 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | PHE524-PHE551 | |
| B | PHE524-PHE551 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 36 |
| Details | TRANSMEM: Helical; Name=9 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | LEU577-GLU595 | |
| B | LEU577-GLU595 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 46 |
| Details | TRANSMEM: Helical; Name=10 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | LEU596-PHE619 | |
| B | LEU596-PHE619 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 38 |
| Details | TRANSMEM: Helical; Name=11 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | ASN637-ASN656 | |
| B | ASN637-ASN656 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 30 |
| Details | TRANSMEM: Helical; Name=12 => ECO:0000269|PubMed:31367042, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | TRP657-ILE672 | |
| B | TRP657-ILE672 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 22 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P55011 |
| Chain | Residue | Details |
| A | LEU219 | |
| A | PHE607 | |
| A | TYR611 | |
| B | LEU219 | |
| B | TRP222 | |
| B | PHE294 | |
| B | ALA418 | |
| B | GLY421 | |
| B | ILE422 | |
| B | ALA535 | |
| B | SER538 | |
| A | TRP222 | |
| B | SER539 | |
| B | PHE607 | |
| B | TYR611 | |
| A | PHE294 | |
| A | ALA418 | |
| A | GLY421 | |
| A | ILE422 | |
| A | ALA535 | |
| A | SER538 | |
| A | SER539 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0007744|PDB:6NPH, ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | ASN220 | |
| B | TYR305 | |
| B | PRO417 | |
| B | THR420 | |
| A | ILE221 | |
| A | VAL224 | |
| A | TYR305 | |
| A | PRO417 | |
| A | THR420 | |
| B | ASN220 | |
| B | ILE221 | |
| B | VAL224 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0007744|PDB:6NPL |
| Chain | Residue | Details |
| A | GLY223 | |
| A | MET225 | |
| B | GLY223 | |
| B | MET225 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P55013 |
| Chain | Residue | Details |
| A | LEU1076 | |
| B | LEU1076 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255|PROSITE-ProRule:PRU00498 |
| Chain | Residue | Details |
| A | ASN475 | |
| A | ASN481 | |
| B | ASN475 | |
| B | ASN481 |
