6N7W

Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)

Functional Information from GO Data

Chain	GOid	namespace	contents
H	0000166	molecular_function	nucleotide binding
H	0003676	molecular_function	nucleic acid binding
H	0003677	molecular_function	DNA binding
H	0003824	molecular_function	catalytic activity
H	0003887	molecular_function	DNA-directed DNA polymerase activity
H	0004518	molecular_function	nuclease activity
H	0004527	molecular_function	exonuclease activity
H	0004529	molecular_function	DNA exonuclease activity
H	0005515	molecular_function	protein binding
H	0006259	biological_process	DNA metabolic process
H	0006260	biological_process	DNA replication
H	0006261	biological_process	DNA-templated DNA replication
H	0006302	biological_process	double-strand break repair
H	0008408	molecular_function	3'-5' exonuclease activity
H	0016740	molecular_function	transferase activity
H	0016779	molecular_function	nucleotidyltransferase activity
H	0016787	molecular_function	hydrolase activity
H	0034061	molecular_function	DNA polymerase activity
H	0039693	biological_process	viral DNA genome replication
H	0046872	molecular_function	metal ion binding
H	0090592	biological_process	DNA synthesis involved in DNA replication
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0015035	molecular_function	protein-disulfide reductase activity
I	0030337	molecular_function	DNA polymerase processivity factor activity
I	0045454	biological_process	cell redox homeostasis

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue MG H 4001

Chain	Residue
H	ASP475
H	ALA476
H	ASP654
H	TTP4002

---

site_id	AC2
Number of Residues	14
Details	binding site for residue TTP H 4002

Chain	Residue
H	GLU480
H	HIS506
H	ARG518
H	LYS522
H	TYR526
H	ASP654
H	MG4001
P	2DA25
T	DT53
H	ARG429
H	ASP475
H	ALA476
H	SER477
H	GLY478

---

Functional Information from PROSITE/UniProt

site_id	PS00194
Number of Residues	19
Details	THIOREDOXIN_1 Thioredoxin family active site. LVdFWaeWCGPCKmIapiL

Chain	Residue	Details
I	LEU24-LEU42

---

site_id	PS00447
Number of Residues	20
Details	DNA_POLYMERASE_A DNA polymerase family A signature. RdnAKtfiYGflYgaGdekI

Chain	Residue	Details
H	ARG518-ILE537

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	502
Details	Region: {"description":"Polymerase","evidences":[{"source":"HAMAP-Rule","id":"MF_04101","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9440688","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	76
Details	Region: {"description":"Binding to host TrxA","evidences":[{"source":"PubMed","id":"15795374","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15292168","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_04101","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9440688","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_04101","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9440688","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9914251","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_04101","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9914251","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Active site: {"description":"Nucleophile"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Site: {"description":"Deprotonates C-terminal active site Cys"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Site: {"description":"Contributes to redox potential value"}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---