6MZG

Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0005929	cellular_component	cilium
A	0007017	biological_process	microtubule-based process
A	0016787	molecular_function	hydrolase activity
A	0031514	cellular_component	motile cilium
A	0042995	cellular_component	cell projection
A	0046872	molecular_function	metal ion binding
B	0000166	molecular_function	nucleotide binding
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0001764	biological_process	neuron migration
B	0003924	molecular_function	GTPase activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0007017	biological_process	microtubule-based process
B	0046872	molecular_function	metal ion binding
C	0000166	molecular_function	nucleotide binding
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000278	biological_process	mitotic cell cycle
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0005929	cellular_component	cilium
C	0007017	biological_process	microtubule-based process
C	0016787	molecular_function	hydrolase activity
C	0031514	cellular_component	motile cilium
C	0042995	cellular_component	cell projection
C	0046872	molecular_function	metal ion binding
D	0000166	molecular_function	nucleotide binding
D	0000226	biological_process	microtubule cytoskeleton organization
D	0000278	biological_process	mitotic cell cycle
D	0001764	biological_process	neuron migration
D	0003924	molecular_function	GTPase activity
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005515	molecular_function	protein binding
D	0005525	molecular_function	GTP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005874	cellular_component	microtubule
D	0007017	biological_process	microtubule-based process
D	0046872	molecular_function	metal ion binding
E	0000022	biological_process	mitotic spindle elongation
E	0000776	cellular_component	kinetochore
E	0005737	cellular_component	cytoplasm
E	0005816	cellular_component	spindle pole body
E	0005856	cellular_component	cytoskeleton
E	0005881	cellular_component	cytoplasmic microtubule
E	0007051	biological_process	spindle organization
E	0015631	molecular_function	tubulin binding
E	0030951	biological_process	establishment or maintenance of microtubule cytoskeleton polarity
E	0031109	biological_process	microtubule polymerization or depolymerization
E	0044732	cellular_component	mitotic spindle pole body
E	0046785	biological_process	microtubule polymerization
E	0051010	molecular_function	microtubule plus-end binding
E	0051315	biological_process	attachment of mitotic spindle microtubules to kinetochore
E	0061863	molecular_function	microtubule plus end polymerase
E	0099070	cellular_component	static microtubule bundle
E	1990498	cellular_component	mitotic spindle microtubule
E	1990571	biological_process	meiotic centromere clustering
G	0000166	molecular_function	nucleotide binding
G	0000226	biological_process	microtubule cytoskeleton organization
G	0000278	biological_process	mitotic cell cycle
G	0005200	molecular_function	structural constituent of cytoskeleton
G	0005525	molecular_function	GTP binding
G	0005737	cellular_component	cytoplasm
G	0005856	cellular_component	cytoskeleton
G	0005874	cellular_component	microtubule
G	0005929	cellular_component	cilium
G	0007017	biological_process	microtubule-based process
G	0016787	molecular_function	hydrolase activity
G	0031514	cellular_component	motile cilium
G	0042995	cellular_component	cell projection
G	0046872	molecular_function	metal ion binding
H	0000166	molecular_function	nucleotide binding
H	0000226	biological_process	microtubule cytoskeleton organization
H	0000278	biological_process	mitotic cell cycle
H	0001764	biological_process	neuron migration
H	0003924	molecular_function	GTPase activity
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005515	molecular_function	protein binding
H	0005525	molecular_function	GTP binding
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005874	cellular_component	microtubule
H	0007017	biological_process	microtubule-based process
H	0046872	molecular_function	metal ion binding
I	0000166	molecular_function	nucleotide binding
I	0000226	biological_process	microtubule cytoskeleton organization
I	0000278	biological_process	mitotic cell cycle
I	0005200	molecular_function	structural constituent of cytoskeleton
I	0005525	molecular_function	GTP binding
I	0005737	cellular_component	cytoplasm
I	0005856	cellular_component	cytoskeleton
I	0005874	cellular_component	microtubule
I	0005929	cellular_component	cilium
I	0007017	biological_process	microtubule-based process
I	0016787	molecular_function	hydrolase activity
I	0031514	cellular_component	motile cilium
I	0042995	cellular_component	cell projection
I	0046872	molecular_function	metal ion binding
J	0000166	molecular_function	nucleotide binding
J	0000226	biological_process	microtubule cytoskeleton organization
J	0000278	biological_process	mitotic cell cycle
J	0001764	biological_process	neuron migration
J	0003924	molecular_function	GTPase activity
J	0005200	molecular_function	structural constituent of cytoskeleton
J	0005515	molecular_function	protein binding
J	0005525	molecular_function	GTP binding
J	0005737	cellular_component	cytoplasm
J	0005856	cellular_component	cytoskeleton
J	0005874	cellular_component	microtubule
J	0007017	biological_process	microtubule-based process
J	0046872	molecular_function	metal ion binding
K	0000022	biological_process	mitotic spindle elongation
K	0000776	cellular_component	kinetochore
K	0005737	cellular_component	cytoplasm
K	0005816	cellular_component	spindle pole body
K	0005856	cellular_component	cytoskeleton
K	0005881	cellular_component	cytoplasmic microtubule
K	0007051	biological_process	spindle organization
K	0015631	molecular_function	tubulin binding
K	0030951	biological_process	establishment or maintenance of microtubule cytoskeleton polarity
K	0031109	biological_process	microtubule polymerization or depolymerization
K	0044732	cellular_component	mitotic spindle pole body
K	0046785	biological_process	microtubule polymerization
K	0051010	molecular_function	microtubule plus-end binding
K	0051315	biological_process	attachment of mitotic spindle microtubules to kinetochore
K	0061863	molecular_function	microtubule plus end polymerase
K	0099070	cellular_component	static microtubule bundle
K	1990498	cellular_component	mitotic spindle microtubule
K	1990571	biological_process	meiotic centromere clustering

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue GTP A 600

Chain	Residue
A	GLY10
A	GLY143
A	GLY144
A	THR145
A	GLY146
A	VAL177
A	GLU183
A	ASN206
A	TYR224
A	ASN228
A	MG601
A	GLN11
B	LYS254
A	ALA12
A	GLN15
A	ASP98
A	ALA99
A	ALA100
A	ASN101
A	SER140

---

site_id	AC2
Number of Residues	2
Details	binding site for residue MG A 601

Chain	Residue
A	GLU71
A	GTP600

---

site_id	AC3
Number of Residues	16
Details	binding site for residue GDP B 600

Chain	Residue
B	GLY10
B	GLN11
B	CYS12
B	GLN15
B	ASN101
B	SER140
B	GLY143
B	GLY144
B	THR145
B	GLY146
B	VAL177
B	GLU183
B	ASN206
B	TYR224
B	ASN228
B	MG601

---

site_id	AC4
Number of Residues	3
Details	binding site for residue MG B 601

Chain	Residue
B	GLN11
B	ASP179
B	GDP600

---

site_id	AC5
Number of Residues	21
Details	binding site for residue GTP C 600

Chain	Residue
C	GLY10
C	GLN11
C	ALA12
C	GLN15
C	ASP98
C	ALA99
C	ALA100
C	ASN101
C	SER140
C	GLY143
C	GLY144
C	THR145
C	GLY146
C	VAL177
C	GLU183
C	ASN206
C	TYR224
C	ASN228
C	ILE231
C	MG601
D	LYS254

---

site_id	AC6
Number of Residues	2
Details	binding site for residue MG C 601

Chain	Residue
C	GLU71
C	GTP600

---

site_id	AC7
Number of Residues	16
Details	binding site for residue GDP D 600

Chain	Residue
D	GLY10
D	GLN11
D	CYS12
D	GLN15
D	ASN101
D	SER140
D	GLY143
D	GLY144
D	THR145
D	GLY146
D	VAL177
D	GLU183
D	ASN206
D	TYR224
D	ASN228
D	MG601

---

site_id	AC8
Number of Residues	2
Details	binding site for residue MG D 601

Chain	Residue
D	GLN11
D	GDP600

---

site_id	AC9
Number of Residues	22
Details	binding site for residue GTP G 600

Chain	Residue
G	TYR224
G	ASN228
G	ILE231
G	MG601
H	LYS254
G	GLY10
G	GLN11
G	ALA12
G	GLN15
G	ASP98
G	ALA99
G	ALA100
G	ASN101
G	SER140
G	GLY143
G	GLY144
G	THR145
G	GLY146
G	ILE171
G	VAL177
G	GLU183
G	ASN206

---

site_id	AD1
Number of Residues	2
Details	binding site for residue MG G 601

Chain	Residue
G	GLU71
G	GTP600

---

site_id	AD2
Number of Residues	17
Details	binding site for residue GDP H 600

Chain	Residue
H	GLY10
H	GLN11
H	CYS12
H	GLN15
H	ASN101
H	SER140
H	GLY143
H	GLY144
H	THR145
H	GLY146
H	VAL177
H	ASP179
H	GLU183
H	ASN206
H	TYR224
H	ASN228
H	MG601

---

site_id	AD3
Number of Residues	4
Details	binding site for residue MG H 601

Chain	Residue
H	GLN11
H	ASN101
H	ASP179
H	GDP600

---

site_id	AD4
Number of Residues	20
Details	binding site for residue GTP I 600

Chain	Residue
I	GLY10
I	GLN11
I	ALA12
I	GLN15
I	ASP98
I	ALA99
I	ALA100
I	ASN101
I	SER140
I	GLY143
I	GLY144
I	THR145
I	GLY146
I	VAL177
I	GLU183
I	ASN206
I	TYR224
I	ASN228
I	MG601
J	LYS254

---

site_id	AD5
Number of Residues	2
Details	binding site for residue MG I 601

Chain	Residue
I	GLU71
I	GTP600

---

site_id	AD6
Number of Residues	17
Details	binding site for residue GDP J 600

Chain	Residue
J	GLY10
J	GLN11
J	CYS12
J	GLN15
J	ASN101
J	SER140
J	GLY143
J	GLY144
J	THR145
J	GLY146
J	PRO173
J	VAL177
J	GLU183
J	ASN206
J	TYR224
J	ASN228
J	MG601

---

site_id	AD7
Number of Residues	2
Details	binding site for residue MG J 601

Chain	Residue
J	GLN11
J	GDP600

---

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
B	GLY142-GLY148
A	GLY142-GLY148

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
B	MET1-ILE4

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	28
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q13509

Chain	Residue	Details
B	GLN11
D	GLY144
D	THR145
D	GLY146
D	ASN206
D	ASN228
H	GLN11
H	SER140
H	GLY144
H	THR145
H	GLY146
B	SER140
H	ASN206
H	ASN228
J	GLN11
J	SER140
J	GLY144
J	THR145
J	GLY146
J	ASN206
J	ASN228
B	GLY144
B	THR145
B	GLY146
B	ASN206
B	ASN228
D	GLN11
D	SER140

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
B	GLU71
C	GLU71
C	SER140
C	GLY144
C	THR145
C	THR179
C	ASN206
C	ASN228
G	GLN11
G	GLU71
G	SER140
D	GLU71
G	GLY144
G	THR145
G	THR179
G	ASN206
G	ASN228
I	GLN11
I	GLU71
I	SER140
I	GLY144
I	THR145
H	GLU71
I	THR179
I	ASN206
I	ASN228
J	GLU71
A	THR145
A	THR179
A	ASN206
A	ASN228
C	GLN11

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
B	SER40
D	SER40
H	SER40
J	SER40

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
B	LYS60
D	LYS60
H	LYS60
J	LYS60

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine; by CDK1 => ECO:0000250|UniProtKB:Q13885

Chain	Residue	Details
B	SER174
D	SER174
H	SER174
J	SER174

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	THR287
B	THR292
D	THR287
D	THR292
H	THR287
H	THR292
J	THR287
J	THR292

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Omega-N-methylarginine => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	ARG320
D	ARG320
H	ARG320
J	ARG320

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:Q2T9S0

Chain	Residue	Details
B	GLU448
D	GLU448
H	GLU448
J	GLU448

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	LYS60
D	LYS60
H	LYS60
J	LYS60

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	LYS326
D	LYS326
H	LYS326
J	LYS326

---