6MZG
Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.130, 194.990, 149.570 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 57.565 - 3.208 |
| R-factor | 0.1802 |
| Rwork | 0.175 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.501 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 149.571 | 57.565 | 3.370 |
| High resolution limit [Å] | 3.200 | 10.120 | 3.200 |
| Rmerge | 0.029 | 0.654 | |
| Rmeas | 0.186 | 0.035 | 0.961 |
| Rpim | 0.105 | 0.019 | 0.547 |
| Number of reflections | 104265 | 3325 | 15435 |
| <I/σ(I)> | 5.8 | 20.1 | 1.1 |
| Completeness [%] | 96.2 | 94.7 | 97.9 |
| Redundancy | 2.9 | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000 |
