6MWQ
Single particle cryoEM structure of a DARPin-aldolase platform in complex with GFP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004332 | molecular_function | fructose-bisphosphate aldolase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006096 | biological_process | glycolytic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0030335 | biological_process | positive regulation of cell migration |
| A | 0030388 | biological_process | fructose 1,6-bisphosphate metabolic process |
| A | 0031430 | cellular_component | M band |
| A | 0031674 | cellular_component | I band |
| A | 0034316 | biological_process | negative regulation of Arp2/3 complex-mediated actin nucleation |
| A | 0051289 | biological_process | protein homotetramerization |
| B | 0004332 | molecular_function | fructose-bisphosphate aldolase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006096 | biological_process | glycolytic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0030335 | biological_process | positive regulation of cell migration |
| B | 0030388 | biological_process | fructose 1,6-bisphosphate metabolic process |
| B | 0031430 | cellular_component | M band |
| B | 0031674 | cellular_component | I band |
| B | 0034316 | biological_process | negative regulation of Arp2/3 complex-mediated actin nucleation |
| B | 0051289 | biological_process | protein homotetramerization |
| C | 0004332 | molecular_function | fructose-bisphosphate aldolase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006096 | biological_process | glycolytic process |
| C | 0016829 | molecular_function | lyase activity |
| C | 0030335 | biological_process | positive regulation of cell migration |
| C | 0030388 | biological_process | fructose 1,6-bisphosphate metabolic process |
| C | 0031430 | cellular_component | M band |
| C | 0031674 | cellular_component | I band |
| C | 0034316 | biological_process | negative regulation of Arp2/3 complex-mediated actin nucleation |
| C | 0051289 | biological_process | protein homotetramerization |
| D | 0004332 | molecular_function | fructose-bisphosphate aldolase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006096 | biological_process | glycolytic process |
| D | 0016829 | molecular_function | lyase activity |
| D | 0030335 | biological_process | positive regulation of cell migration |
| D | 0030388 | biological_process | fructose 1,6-bisphosphate metabolic process |
| D | 0031430 | cellular_component | M band |
| D | 0031674 | cellular_component | I band |
| D | 0034316 | biological_process | negative regulation of Arp2/3 complex-mediated actin nucleation |
| D | 0051289 | biological_process | protein homotetramerization |
| G | 0003674 | molecular_function | molecular_function |
| G | 0005575 | cellular_component | cellular_component |
| G | 0006091 | biological_process | generation of precursor metabolites and energy |
| G | 0008218 | biological_process | bioluminescence |
| H | 0003674 | molecular_function | molecular_function |
| H | 0005575 | cellular_component | cellular_component |
| H | 0006091 | biological_process | generation of precursor metabolites and energy |
| H | 0008218 | biological_process | bioluminescence |
| I | 0003674 | molecular_function | molecular_function |
| I | 0005575 | cellular_component | cellular_component |
| I | 0006091 | biological_process | generation of precursor metabolites and energy |
| I | 0008218 | biological_process | bioluminescence |
| J | 0003674 | molecular_function | molecular_function |
| J | 0005575 | cellular_component | cellular_component |
| J | 0006091 | biological_process | generation of precursor metabolites and energy |
| J | 0008218 | biological_process | bioluminescence |
Functional Information from PROSITE/UniProt
| site_id | PS00158 |
| Number of Residues | 11 |
| Details | ALDOLASE_CLASS_I Fructose-bisphosphate aldolase class-I active site. IyLEGtLLKPN |
| Chain | Residue | Details |
| A | ILE390-ASN400 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | MOD_RES: (Z)-2,3-didehydrotyrosine => ECO:0000269|PubMed:8448132 |
| Chain | Residue | Details |
| G | GLN65 | |
| J | GLN65 | |
| I | GLN65 | |
| H | GLN65 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | CROSSLNK: 5-imidazolinone (Ser-Gly) => ECO:0000269|PubMed:8448132 |
| Chain | Residue | Details |
| G | VAL64 | |
| G | CYS66 | |
| J | VAL64 | |
| J | CYS66 | |
| I | VAL64 | |
| I | CYS66 | |
| H | VAL64 | |
| H | CYS66 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10504235, ECO:0007744|PDB:6ALD |
| Chain | Residue | Details |
| A | ARG211 | |
| C | SER440 | |
| C | SER469 | |
| C | ARG472 | |
| D | ARG211 | |
| D | SER440 | |
| D | SER469 | |
| D | ARG472 | |
| A | SER440 | |
| A | SER469 | |
| A | ARG472 | |
| B | ARG211 | |
| B | SER440 | |
| B | SER469 | |
| B | ARG472 | |
| C | ARG211 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | SITE: Essential for substrate cleavage |
| Chain | Residue | Details |
| A | CYS241 | |
| A | LYS276 | |
| B | CYS241 | |
| B | LYS276 | |
| C | CYS241 | |
| C | LYS276 | |
| D | CYS241 | |
| D | LYS276 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | SITE: Alkylation inactivates the enzyme |
| Chain | Residue | Details |
| A | LYS315 | |
| B | LYS315 | |
| C | LYS315 | |
| D | LYS315 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 16 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P04075 |
| Chain | Residue | Details |
| A | SER204 | |
| C | SER207 | |
| C | SER214 | |
| C | SER440 | |
| D | SER204 | |
| D | SER207 | |
| D | SER214 | |
| D | SER440 | |
| A | SER207 | |
| A | SER214 | |
| A | SER440 | |
| B | SER204 | |
| B | SER207 | |
| B | SER214 | |
| B | SER440 | |
| C | SER204 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P04075 |
| Chain | Residue | Details |
| A | LYS210 | |
| B | LYS210 | |
| C | LYS210 | |
| D | LYS210 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 8 |
| Details | MOD_RES: N6-(2-hydroxyisobutyryl)lysine => ECO:0000250|UniProtKB:P04075 |
| Chain | Residue | Details |
| A | LYS267 | |
| A | LYS315 | |
| B | LYS267 | |
| B | LYS315 | |
| C | LYS267 | |
| C | LYS315 | |
| D | LYS267 | |
| D | LYS315 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 8 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P04075 |
| Chain | Residue | Details |
| A | LYS276 | |
| A | LYS498 | |
| B | LYS276 | |
| B | LYS498 | |
| C | LYS276 | |
| C | LYS498 | |
| D | LYS276 | |
| D | LYS498 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-malonyllysine; alternate => ECO:0000250 |
| Chain | Residue | Details |
| A | LYS279 | |
| B | LYS279 | |
| C | LYS279 | |
| D | LYS279 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P05065 |
| Chain | Residue | Details |
| A | SER300 | |
| B | SER300 | |
| C | SER300 | |
| D | SER300 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-malonyllysine => ECO:0000250 |
| Chain | Residue | Details |
| A | LYS480 | |
| B | LYS480 | |
| C | LYS480 | |
| D | LYS480 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0000250|UniProtKB:P04075 |
| Chain | Residue | Details |
| A | LYS210 | |
| B | LYS210 | |
| C | LYS210 | |
| D | LYS210 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 222 |
| Chain | Residue | Details |
| A | ASP202 | electrostatic stabiliser, hydrogen bond acceptor |
| A | LYS315 | electrostatic stabiliser, hydrogen bond donor |
| A | GLU356 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, polar interaction, proton acceptor, proton donor, proton relay |
| A | GLU358 | activator, electrostatic stabiliser, hydrogen bond acceptor, polar interaction |
| A | LYS398 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor, proton relay |
| A | SER469 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 222 |
| Chain | Residue | Details |
| B | ASP202 | electrostatic stabiliser, hydrogen bond acceptor |
| B | LYS315 | electrostatic stabiliser, hydrogen bond donor |
| B | GLU356 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, polar interaction, proton acceptor, proton donor, proton relay |
| B | GLU358 | activator, electrostatic stabiliser, hydrogen bond acceptor, polar interaction |
| B | LYS398 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor, proton relay |
| B | SER469 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor |
| site_id | MCSA3 |
| Number of Residues | 6 |
| Details | M-CSA 222 |
| Chain | Residue | Details |
| C | ASP202 | electrostatic stabiliser, hydrogen bond acceptor |
| C | LYS315 | electrostatic stabiliser, hydrogen bond donor |
| C | GLU356 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, polar interaction, proton acceptor, proton donor, proton relay |
| C | GLU358 | activator, electrostatic stabiliser, hydrogen bond acceptor, polar interaction |
| C | LYS398 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor, proton relay |
| C | SER469 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor |
| site_id | MCSA4 |
| Number of Residues | 6 |
| Details | M-CSA 222 |
| Chain | Residue | Details |
| D | ASP202 | electrostatic stabiliser, hydrogen bond acceptor |
| D | LYS315 | electrostatic stabiliser, hydrogen bond donor |
| D | GLU356 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, polar interaction, proton acceptor, proton donor, proton relay |
| D | GLU358 | activator, electrostatic stabiliser, hydrogen bond acceptor, polar interaction |
| D | LYS398 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor, proton relay |
| D | SER469 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor |
